Proteome-Wide Systems Analysis of a Cellulosic Biofuel-Producing Microbe
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Chilaka, Amanda C
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https://doi.org/10.1038/msb.2010.116Metadata
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Tolonen, Andrew C., Wilhelm Haas, Amanda C. Chilaka, John Aach, Steven P. Gygi, and George M. Church. 2011. Proteome-wide systems analysis of a cellulosic biofuel-producing microbe. Molecular Systems Biology 7: 461.Abstract
Fermentation of plant biomass by microbes like Clostridium phytofermentans recycles carbon globally and can make biofuels from inedible feedstocks. We analyzed C. phytofermentans fermenting cellulosic substrates by integrating quantitative mass spectrometry of more than 2500 proteins with measurements of growth, enzyme activities, fermentation products, and electron microscopy. Absolute protein concentrations were estimated using Absolute Protein EXpression (APEX); relative changes between treatments were quantified with chemical stable isotope labeling by reductive dimethylation (ReDi). We identified the different combinations of carbohydratases used to degrade cellulose and hemicellulose, many of which were secreted based on quantification of supernatant proteins, as well as the repertoires of glycolytic enzymes and alcohol dehydrogenases (ADHs) enabling ethanol production at near maximal yields. Growth on cellulose also resulted in diverse changes such as increased expression of tryptophan synthesis proteins and repression of proteins for fatty acid metabolism and cell motility. This study gives a systems-level understanding of how this microbe ferments biomass and provides a rational, empirical basis to identify engineering targets for industrial cellulosic fermentation.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3049413/pdf/Terms of Use
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