Proteome-Wide Systems Analysis of a Cellulosic Biofuel-Producing Microbe

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Proteome-Wide Systems Analysis of a Cellulosic Biofuel-Producing Microbe

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dc.contributor.author Chilaka, Amanda C
dc.contributor.author Tolonen, Andrew
dc.contributor.author Haas, Wilhelm
dc.contributor.author Aach, John Dennis
dc.contributor.author Gygi, Steven P.
dc.contributor.author Church, George McDonald
dc.date.accessioned 2011-12-21T22:07:59Z
dc.date.issued 2011
dc.identifier.citation Tolonen, Andrew C., Wilhelm Haas, Amanda C. Chilaka, John Aach, Steven P. Gygi, and George M. Church. 2011. Proteome-wide systems analysis of a cellulosic biofuel-producing microbe. Molecular Systems Biology 7: 461. en_US
dc.identifier.issn 1744-4292 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:5697570
dc.description.abstract Fermentation of plant biomass by microbes like Clostridium phytofermentans recycles carbon globally and can make biofuels from inedible feedstocks. We analyzed C. phytofermentans fermenting cellulosic substrates by integrating quantitative mass spectrometry of more than 2500 proteins with measurements of growth, enzyme activities, fermentation products, and electron microscopy. Absolute protein concentrations were estimated using Absolute Protein EXpression (APEX); relative changes between treatments were quantified with chemical stable isotope labeling by reductive dimethylation (ReDi). We identified the different combinations of carbohydratases used to degrade cellulose and hemicellulose, many of which were secreted based on quantification of supernatant proteins, as well as the repertoires of glycolytic enzymes and alcohol dehydrogenases (ADHs) enabling ethanol production at near maximal yields. Growth on cellulose also resulted in diverse changes such as increased expression of tryptophan synthesis proteins and repression of proteins for fatty acid metabolism and cell motility. This study gives a systems-level understanding of how this microbe ferments biomass and provides a rational, empirical basis to identify engineering targets for industrial cellulosic fermentation. en_US
dc.language.iso en_US en_US
dc.publisher Nature Publishing Group en_US
dc.relation.isversionof doi://10.1038/msb.2010.116 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3049413/pdf/ en_US
dash.license LAA
dc.title Proteome-Wide Systems Analysis of a Cellulosic Biofuel-Producing Microbe en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Molecular Systems Biology en_US
dash.depositing.author Gygi, Steven P.
dc.date.available 2011-12-21T22:07:59Z
dash.affiliation.other HMS^Genetics en_US
dash.affiliation.other HMS^Genetics en_US
dash.affiliation.other HMS^Cell Biology en_US
dash.affiliation.other HMS^Health Sciences and Technology en_US
dash.affiliation.other HMS^Genetics en_US

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