A Conserved CCCH-type Zinc Finger Protein Regulates mRNA Nuclear Adenylation and Export

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A Conserved CCCH-type Zinc Finger Protein Regulates mRNA Nuclear Adenylation and Export

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Title: A Conserved CCCH-type Zinc Finger Protein Regulates mRNA Nuclear Adenylation and Export
Author: Hurt, Jessica A.; Farny, Natalie G.; Obar, Robert Alan; Zhai, Bo; Gygi, Steven P.; Silver, Pamela A.

Note: Order does not necessarily reflect citation order of authors.

Citation: Hurt, Jessica A., Robert A. Obar, Bo Zhai, Natalie G. Farny, Steven P. Gygi, and Pamela A. Silver. 2009. A conserved CCCH-type zinc finger protein regulates mRNA nuclear adenylation and export. The Journal of Cell Biology 185(2): 265-277.
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Abstract: Coupling of messenger RNA (mRNA) nuclear export with prior processing steps aids in the fidelity and efficiency of mRNA transport to the cytoplasm. In this study, we show that the processes of export and polyadenylation are coupled via the Drosophila melanogaster CCCH-type zinc finger protein CG6694/dZC3H3 through both physical and functional interactions. We show that depletion of dZC3H3 from S2R+ cells results in transcript hyperadenylation. Using targeted coimmunoprecipitation and liquid chromatography mass spectrometry (MS)/MS techniques, we characterize interactions of known components of the mRNA nuclear export and polyadenylation machineries with dZC3H3. Furthermore, we demonstrate the functional conservation of this factor, as depletion of its human homologue ZC3H3 by small interfering RNA results in an mRNA export defect in human cells as well. Nuclear polyadenylated (poly(A)) RNA in ZC3H3-depleted cells is sequestered in foci removed from SC35-containing speckles, indicating a shift from the normal subnuclear distribution of poly(A) RNA. Our data suggest a model wherein ZC3H3 interfaces between the polyadenylation machinery, newly poly(A) mRNAs, and factors for transcript export.
Published Version: doi://10.1083/jcb.200811072
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2700372/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:5765206

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