Kinetic Analysis of Protein Aggregation Monitored by Real-Time 2D Solid-State NMR Spectroscopy

Kinetic Analysis of Protein Aggregation Monitored by Real-Time 2D Solid-State NMR Spectroscopy

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Kinetic Analysis of Protein Aggregation Monitored by Real-Time 2D Solid-State NMR Spectroscopy

dc.contributor.author	Baldus, Marc
dc.contributor.author	Etzkorn, Manuel
dc.contributor.author	Bockmann, Anja
dc.date.accessioned	2012-01-09T18:00:32Z
dc.date.issued	2011
dc.identifier.citation	Etzkorn, Manuel, Anja Bockmann, and Marc Baldus. 2011. Kinetic analysis of protein aggregation monitored by real-time 2D solid-state NMR spectroscopy. Journal of Biomolecular NMR 49(2): 121-129.	en_US
dc.identifier.issn	0925-2738	en_US
dc.identifier.uri	http://nrs.harvard.edu/urn-3:HUL.InstRepos:7349755
dc.description.abstract	It is shown that real-time 2D solid-state NMR can be used to obtain kinetic and structural information about the process of protein aggregation. In addition to the incorporation of kinetic information involving intermediate states, this approach can offer atom-specific resolution for all detectable species. The analysis was carried out using experimental data obtained during aggregation of the 10.4 kDa Crh protein, which has been shown to involve a partially unfolded intermediate state prior to aggregation. Based on a single real-time 2D \(^{13}\textrm{C}–^{13}\textrm{C}\) transition spectrum, kinetic information about the refolding and aggregation step could be extracted. In addition, structural rearrangements associated with refolding are estimated and several different aggregation scenarios were compared to the experimental data.	en_US
dc.language.iso	en_US	en_US
dc.publisher	Springer Netherlands	en_US
dc.relation.isversionof	doi:10.1007/s10858-011-9468-6	en_US
dc.relation.hasversion	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3042102/pdf/	en_US
dash.license	LAA
dc.subject	aggregation	en_US
dc.subject	kinetic	en_US
dc.subject	solid-state NMR	en_US
dc.subject	real-time spectroscopy	en_US
dc.subject	Crh	en_US
dc.title	Kinetic Analysis of Protein Aggregation Monitored by Real-Time 2D Solid-State NMR Spectroscopy	en_US
dc.type	Journal Article	en_US
dc.description.version	Version of Record	en_US
dc.relation.journal	Journal of Biomolecular NMR	en_US
dash.depositing.author	Etzkorn, Manuel
dc.date.available	2012-01-09T18:00:32Z
dash.affiliation.other	100160	en_US