Kinetic Analysis of Protein Aggregation Monitored by Real-Time 2D Solid-State NMR Spectroscopy

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Kinetic Analysis of Protein Aggregation Monitored by Real-Time 2D Solid-State NMR Spectroscopy

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dc.contributor.author Baldus, Marc
dc.contributor.author Etzkorn, Manuel
dc.contributor.author Bockmann, Anja
dc.date.accessioned 2012-01-09T18:00:32Z
dc.date.issued 2011
dc.identifier.citation Etzkorn, Manuel, Anja Bockmann, and Marc Baldus. 2011. Kinetic analysis of protein aggregation monitored by real-time 2D solid-state NMR spectroscopy. Journal of Biomolecular NMR 49(2): 121-129. en_US
dc.identifier.issn 0925-2738 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:7349755
dc.description.abstract It is shown that real-time 2D solid-state NMR can be used to obtain kinetic and structural information about the process of protein aggregation. In addition to the incorporation of kinetic information involving intermediate states, this approach can offer atom-specific resolution for all detectable species. The analysis was carried out using experimental data obtained during aggregation of the 10.4 kDa Crh protein, which has been shown to involve a partially unfolded intermediate state prior to aggregation. Based on a single real-time 2D \(^{13}\textrm{C}–^{13}\textrm{C}\) transition spectrum, kinetic information about the refolding and aggregation step could be extracted. In addition, structural rearrangements associated with refolding are estimated and several different aggregation scenarios were compared to the experimental data. en_US
dc.language.iso en_US en_US
dc.publisher Springer Netherlands en_US
dc.relation.isversionof doi:10.1007/s10858-011-9468-6 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3042102/pdf/ en_US
dash.license LAA
dc.subject aggregation en_US
dc.subject kinetic en_US
dc.subject solid-state NMR en_US
dc.subject real-time spectroscopy en_US
dc.subject Crh en_US
dc.title Kinetic Analysis of Protein Aggregation Monitored by Real-Time 2D Solid-State NMR Spectroscopy en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Journal of Biomolecular NMR en_US
dash.depositing.author Etzkorn, Manuel
dc.date.available 2012-01-09T18:00:32Z
dash.affiliation.other 100160 en_US

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