MicroRNA-1 and MicroRNA-206 Regulate Skeletal Muscle Satellite Cell Proliferation and Differentiation by Repressing Pax7
DSpace/Manakin Repository
MicroRNA-1 and MicroRNA-206 Regulate Skeletal Muscle Satellite Cell Proliferation and Differentiation by Repressing Pax7
| Title: | MicroRNA-1 and MicroRNA-206 Regulate Skeletal Muscle Satellite Cell Proliferation and Differentiation by Repressing Pax7 |
| Author: |
Chen, Jian-Fu; Tao, Yazhong; Deng, Zhongliang; Xiao, Xiao; Wang, Da-Zhi; Yan, Zhen; Li, Juan
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Chen, Jian-Fu, Yazhong Tao, Juan Li, Zhongliang Deng, Zhen Yan, Xiao Xiao, and Da-Zhi Wang. 2010. microRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7. The Journal of Cell Biology 190(5): 867-879. |
| Full Text & Related Files: |
2935565.pdf (3.152Mb; PDF) 
|
| Abstract: | Skeletal muscle satellite cells are adult stem cells responsible for postnatal skeletal muscle growth and regeneration. Paired-box transcription factor Pax7 plays a central role in satellite cell survival, self-renewal, and proliferation. However, how Pax7 is regulated during the transition from proliferating satellite cells to differentiating myogenic progenitor cells is largely unknown. In this study, we find that miR-1 and miR-206 are sharply up-regulated during satellite cell differentiation and down-regulated after muscle injury. We show that miR-1 and miR-206 facilitate satellite cell differentiation by restricting their proliferative potential. We identify Pax7 as one of the direct regulatory targets of miR-1 and miR-206. Inhibition of miR-1 and miR-206 substantially enhances satellite cell proliferation and increases Pax7 protein level in vivo. Conversely, sustained Pax7 expression as a result of the loss of miR-1 and miR-206 repression elements at its 3′ untranslated region significantly inhibits myoblast differentiation. Therefore, our experiments suggest that microRNAs participate in a regulatory circuit that allows rapid gene program transitions from proliferation to differentiation. |
| Published Version: | doi://10.1083/jcb.200911036 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935565/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:7351761 |
This item appears in the following Collection(s)
-
HMS Scholarly Articles [4000]
Contact administrator regarding this item (to report mistakes or request changes)
