MicroRNA-1 and MicroRNA-206 Regulate Skeletal Muscle Satellite Cell Proliferation and Differentiation by Repressing Pax7

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MicroRNA-1 and MicroRNA-206 Regulate Skeletal Muscle Satellite Cell Proliferation and Differentiation by Repressing Pax7

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Title: MicroRNA-1 and MicroRNA-206 Regulate Skeletal Muscle Satellite Cell Proliferation and Differentiation by Repressing Pax7
Author: Chen, Jian-Fu; Tao, Yazhong; Deng, Zhongliang; Xiao, Xiao; Wang, Da-Zhi; Yan, Zhen; Li, Juan

Note: Order does not necessarily reflect citation order of authors.

Citation: Chen, Jian-Fu, Yazhong Tao, Juan Li, Zhongliang Deng, Zhen Yan, Xiao Xiao, and Da-Zhi Wang. 2010. microRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7. The Journal of Cell Biology 190(5): 867-879.
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Abstract: Skeletal muscle satellite cells are adult stem cells responsible for postnatal skeletal muscle growth and regeneration. Paired-box transcription factor Pax7 plays a central role in satellite cell survival, self-renewal, and proliferation. However, how Pax7 is regulated during the transition from proliferating satellite cells to differentiating myogenic progenitor cells is largely unknown. In this study, we find that miR-1 and miR-206 are sharply up-regulated during satellite cell differentiation and down-regulated after muscle injury. We show that miR-1 and miR-206 facilitate satellite cell differentiation by restricting their proliferative potential. We identify Pax7 as one of the direct regulatory targets of miR-1 and miR-206. Inhibition of miR-1 and miR-206 substantially enhances satellite cell proliferation and increases Pax7 protein level in vivo. Conversely, sustained Pax7 expression as a result of the loss of miR-1 and miR-206 repression elements at its 3′ untranslated region significantly inhibits myoblast differentiation. Therefore, our experiments suggest that microRNAs participate in a regulatory circuit that allows rapid gene program transitions from proliferation to differentiation.
Published Version: doi://10.1083/jcb.200911036
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935565/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:7351761

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