A Quantitative Test of Population Genetics Using Spatio-Genetic Patterns in Bacterial Colonies

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A Quantitative Test of Population Genetics Using Spatio-Genetic Patterns in Bacterial Colonies

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dc.contributor.author Korolev, Kirill
dc.contributor.author Xavier, Joao
dc.contributor.author Foster, Kevin
dc.contributor.author Nelson, David R.
dc.date.accessioned 2012-01-19T19:38:20Z
dc.date.issued 2011
dc.identifier.citation Korolev, Kirill, Joao B. Xavier, David R. Nelson, and Kevin R. Foster. 2011. A quantitative test of population genetics using spatio-genetic patterns in bacterial colonies. The American Naturalist 178(4): 538-552. en_US
dc.identifier.issn 1537-5323 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:7982715
dc.description.abstract It is widely accepted that population genetics theory is the cornerstone of evolutionary analyses. Empirical tests of the theory, however, are challenging because of the complex relationships between space, dispersal, and evolution. Critically, we lack quantitative validation of the spatial models of population genetics. Here we combine analytics, on and off-lattice simulations, and experiments with bacteria to perform quantitative tests of the theory. We study two bacterial species, the gut microbe Escherichia coli and the opportunistic pathogen Pseudomonas aeruginosa, and show that spatio-genetic patterns in colony biofilms of both species are accurately described by an extension of the one-dimensional stepping-stone model. We use one empirical measure, genetic diversity at the colony periphery, to parameterize our models and show that we can then accurately predict another key variable: the degree of short-range cell migration along an edge. Moreover, the model allows us to estimate other key parameters including effective population size (density) at the expansion frontier. While our experimental system is a simplification of natural microbial community, we argue it is a proof of principle that the spatial models of population genetics can quantitatively capture organismal evolution. en_US
dc.description.sponsorship Physics en_US
dc.language.iso en_US en_US
dc.publisher University of Chicago Press en_US
dc.relation.isversionof doi:10.1086/661897 en_US
dc.relation.hasversion http://arxiv.org/abs/1110.5376v1 en_US
dash.license OAP
dc.subject spatial population genetics en_US
dc.subject genetic drift en_US
dc.subject range expansion en_US
dc.subject neutral evolution en_US
dc.subject bacterial colonies en_US
dc.subject biofilms en_US
dc.subject populations and evolution en_US
dc.subject statistical mechanics en_US
dc.title A Quantitative Test of Population Genetics Using Spatio-Genetic Patterns in Bacterial Colonies en_US
dc.type Journal Article en_US
dc.description.version Author's Original en_US
dc.relation.journal The American Naturalist en_US
dash.depositing.author Nelson, David R.
dc.date.available 2012-01-19T19:38:20Z

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  • FAS Scholarly Articles [6463]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University

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