Preferential Binding of HIF-1 to Transcriptionally Active Loci Determines Cell-type Specific Response to Hypoxia

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Preferential Binding of HIF-1 to Transcriptionally Active Loci Determines Cell-type Specific Response to Hypoxia

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Title: Preferential Binding of HIF-1 to Transcriptionally Active Loci Determines Cell-type Specific Response to Hypoxia
Author: Xia, Xiaobo; Kung, Andrew Li-Jen

Note: Order does not necessarily reflect citation order of authors.

Citation: Xia, Xiaobo, and Andrew L Kung. 2009. Preferential binding of HIF-1 to transcriptionally active loci determines cell-type specific response to hypoxia. Genome Biology 10(10): R113.
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Abstract: Background: Hypoxia-inducible factor 1 (HIF-1) plays a key role in cellular adaptation to hypoxia. To better understand the determinants of HIF-1 binding and transactivation, we used ChIP-chip and gene expression profiling to define the relationship between the epigenetic landscape, sites of HIF-1 binding, and genes transactivated by hypoxia in two cell lines. Results: We found that when cells were acutely subjected to hypoxia, HIF-1 preferentially bound to loci that were already transcriptionally active under normal growth conditions characterized by the presence of histone H3 lysine 4 methylation, the presence of RNA polymerase II, and basal production of mRNA. Cell type-specific differences in HIF-1 binding were largely attributable to differences in the basal gene expression patterns in the cells prior to the onset of hypoxia. Conclusions: These results suggest that the repertoire of genes active in a cell (for example, through lineage specific transcription factors) defines the subset of genes that are permissive for binding and transactivation by stimulus-responsive transcription factors.
Published Version: doi://10.1186/gb-2009-10-10-r113
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2784328/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:8000932

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