Fluidization and Resolidification of the Human Bladder Smooth Muscle Cell in Response to Transient Stretch

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Fluidization and Resolidification of the Human Bladder Smooth Muscle Cell in Response to Transient Stretch

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dc.contributor.author Rajendran, Kavitha
dc.contributor.author Deng, Linhong
dc.contributor.author Sokolov, Igor
dc.contributor.author Chen, Cheng
dc.contributor.author Krishnan, Ramaswamy
dc.contributor.author Zhou, Enhua
dc.contributor.author Ramachandran, Aruna
dc.contributor.author Tambe, Dhananjay T
dc.contributor.author Adam, Rosalyn Mare
dc.contributor.author Fredberg, Jeffrey J.
dc.date.accessioned 2012-01-29T19:17:53Z
dc.date.issued 2010
dc.identifier.citation Chen, Cheng, Ramaswamy Krishnan, Enhua Zhou, Aruna Ramachandran, Dhananjay Tambe, Kavitha Rajendran, Rosalyn M. Adam, Linhong Deng, and Jeffrey J. Fredberg. 2010. Fluidization and resolidification of the human bladder smooth muscle cell in response to transient stretch. PLoS ONE 5(8): e12035. en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:8063392
dc.description.abstract Background: Cells resident in certain hollow organs are subjected routinely to large transient stretches, including every adherent cell resident in lungs, heart, great vessels, gut, and bladder. We have shown recently that in response to a transient stretch the adherent eukaryotic cell promptly fluidizes and then gradually resolidifies, but mechanism is not yet understood. Principal Findings: In the isolated human bladder smooth muscle cell, here we applied a 10% transient stretch while measuring cell traction forces, elastic modulus, F-actin imaging and the F-actin/G-actin ratio. Immediately after a transient stretch, F-actin levels and cell stiffness were lower by about 50%, and traction forces were lower by about 70%, both indicative of prompt fluidization. Within 5min, F-actin levels recovered completely, cell stiffness recovered by about 90%, and traction forces recovered by about 60%, all indicative of resolidification. The extent of the fluidization response was uninfluenced by a variety of signaling inhibitors, and, surprisingly, was localized to the unstretch phase of the stretch-unstretch maneuver in a manner suggestive of cytoskeletal catch bonds. When we applied an “unstretch-restretch” (transient compression), rather than a “stretch-unstretch” (transient stretch), the cell did not fluidize and the actin network did not depolymerize. Conclusions: Taken together, these results implicate extremely rapid actin disassembly in the fluidization response, and slow actin reassembly in the resolidification response. In the bladder smooth muscle cell, the fluidization response to transient stretch occurs not through signaling pathways, but rather through release of increased tensile forces that drive acute disassociation of actin. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi://10.1371/journal.pone.0012035 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2917357/pdf/ en_US
dash.license LAA
dc.subject biophysics en_US
dc.subject cell biology en_US
dc.subject physics en_US
dc.subject condensed matter en_US
dc.title Fluidization and Resolidification of the Human Bladder Smooth Muscle Cell in Response to Transient Stretch en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS ONE en_US
dash.depositing.author Krishnan, Ramaswamy
dc.date.available 2012-01-29T19:17:53Z
dash.affiliation.other HMS^Financial Operations and Analysis en_US
dash.affiliation.other SPH^Molecular+Integrative Physiological Sci Prog en_US
dash.affiliation.other SPH^Molecular+Integrative Physiological Sci Prog en_US

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