Probing Dynein and Kinesin Stepping with Mechanical Manipulation in a Living Cell

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Probing Dynein and Kinesin Stepping with Mechanical Manipulation in a Living Cell

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Title: Probing Dynein and Kinesin Stepping with Mechanical Manipulation in a Living Cell
Author: Sims, Peter Alan; Xie, Xiaoliang Sunney

Note: Order does not necessarily reflect citation order of authors.

Citation: Sims, Peter Alan and Xiaoliang Sunney Xie. 2009. Probing dynein and kinesin stepping with mechanical manipulation in a living cell. Chemphyschem 10(9-10): 1511-1516.
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Abstract: Molecular motors: By combining optical tweezers and high-speed particle tracking, individual steps of microtubule motor proteins transporting organelles can be detected under known force loads in living mammalian cells (see figure). We report a label-free assay for simultaneous optical manipulation and tracking of endogenous lipid droplets as actively transported cargoes in a living mammalian cell with sub-millisecond time resolution. Using an EM-CCD camera as a highly sensitive quadrant detector, we can detect steps of dynein- and kinesin-driven cargoes under known force loads. We can distinguish single and multiple motor-driven cargoes and show that the stall forces for inward and outward transported cargoes are similar. By combining the stall force observable with the ability to detect individual steps, we can characterize kinesin- and dynein-driven active transport in different force regimes.
Published Version: doi:10.1002/cphc.200900113
Other Sources: http://bernstein.harvard.edu/papers/PS_chemphyschem_2009.pdf
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:8152052

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  • FAS Scholarly Articles [6929]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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