Ultrasound-Guided In Utero Injections Allow Studies of the Development and Function of the Eye
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Ultrasound-Guided In Utero Injections Allow Studies of the Development and Function of the Eye
| Title: | Ultrasound-Guided In Utero Injections Allow Studies of the Development and Function of the Eye |
| Author: |
Punzo, Claudio; Cepko, Connie
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Punzo, Claudio, and Constance L. Cepko. 2008. Ultrasound-guided in utero injections allow studies of the development and function of the eye. Developmental Dynamics 237 (4): 1034-1042. |
| Full Text & Related Files: |
2532702.pdf (484.2Kb; PDF) 
|
| Abstract: | Ultrasound-guided in utero injections into the brain of murine embryos has been shown to facilitate gene delivery. We investigated whether these methods would allow gene transfer into ocular structures. Gene transfer using retroviral vectors or electroporation was found to be quite effective. We determined the window of time, as well as compared several strains of mice, that yield a high degree of survival and successful gene transfer. Several retroviral constructs were tested for expression and coexpresssion of two genes in retinal cell types. In addition, a retroviral vector was engineered to give cone photoreceptor-enriched expression, and a retroviral vector was demonstrated to provide RNAi-mediated loss-of-function. These methods enable access to early ocular structures and provide a more rapid method of assessment of gene and promoter function than possible using genetically engineered mice. |
| Published Version: | doi://10.1002/dvdy.21500 |
| Other Sources: |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2532702/pdf/
www.interscience.wiley.com |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:8160858 |
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