Ultrasound-Guided In Utero Injections Allow Studies of the Development and Function of the Eye

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Ultrasound-Guided In Utero Injections Allow Studies of the Development and Function of the Eye

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dc.contributor.author Punzo, Claudio
dc.contributor.author Cepko, Connie
dc.date.accessioned 2012-02-14T00:39:21Z
dc.date.issued 2008
dc.identifier.citation Punzo, Claudio, and Constance L. Cepko. 2008. Ultrasound-guided in utero injections allow studies of the development and function of the eye. Developmental Dynamics 237 (4): 1034-1042. en_US
dc.identifier.issn 1058-8388 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:8160858
dc.description.abstract Ultrasound-guided in utero injections into the brain of murine embryos has been shown to facilitate gene delivery. We investigated whether these methods would allow gene transfer into ocular structures. Gene transfer using retroviral vectors or electroporation was found to be quite effective. We determined the window of time, as well as compared several strains of mice, that yield a high degree of survival and successful gene transfer. Several retroviral constructs were tested for expression and coexpresssion of two genes in retinal cell types. In addition, a retroviral vector was engineered to give cone photoreceptor-enriched expression, and a retroviral vector was demonstrated to provide RNAi-mediated loss-of-function. These methods enable access to early ocular structures and provide a more rapid method of assessment of gene and promoter function than possible using genetically engineered mice. en_US
dc.language.iso en_US en_US
dc.publisher Wiley-Liss, Inc. en_US
dc.relation.isversionof doi://10.1002/dvdy.21500 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2532702/pdf/ en_US
dc.relation.hasversion www.interscience.wiley.com en_US
dash.license LAA
dc.subject ultrasound en_US
dc.subject eye development en_US
dc.subject mouse en_US
dc.subject gain and loss-of-function clones en_US
dc.subject retina en_US
dc.subject clonal analysis en_US
dc.title Ultrasound-Guided In Utero Injections Allow Studies of the Development and Function of the Eye en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Developmental Dynamics en_US
dash.depositing.author Cepko, Connie
dc.date.available 2012-02-14T00:39:21Z
dash.affiliation.other HMS^Ophthalmology en_US
dash.affiliation.other HMS^Genetics en_US

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