Arthritis Imaging Using a Near-Infrared Fluorescence Folate-Targeted Probe
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Arthritis Imaging Using a Near-Infrared Fluorescence Folate-Targeted Probe
| Title: | Arthritis Imaging Using a Near-Infrared Fluorescence Folate-Targeted Probe |
| Author: |
Chen, Wei-Tsung; Tung, Ching-Hsuan; Mahmood, Umar; Weissleder, Ralph
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Chen, Wei-Tsung, Umar Mahmood, Ralph Weissleder, and Ching-Hsuan Tung. 2005. Arthritis imaging using a near-infrared fluorescence folate-targeted probe. Arthritis Research & Therapy 7(2): R310-R317. |
| Full Text & Related Files: |
1065321.pdf (2.380Mb; PDF) 
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| Abstract: | A recently developed near-infrared fluorescence-labeled folate probe (NIR2-folate) was tested for in vivo imaging of arthritis using a lipopolysaccharide intra-articular injection model and a KRN transgenic mice serum induction mouse model. In the lipopolysaccharide injection model, the fluorescence signal intensity of NIR2-folate (n = 12) and of free NIR2 (n = 5) was compared between lipopolysaccharide-treated and control joints. The fluorescence signal intensity of the NIR2-folate probe at the inflammatory joints was found to be significantly higher than the control normal joints (up to 2.3-fold, P < 0.001). The NIR2-free dye injection group showed a persistent lower enhancement ratio than the NIR2-folate probe injection group. Excessive folic acid was also given to demonstrate a competitive effect with the NIR2-folate. In the KRN serum transfer model (n = 4), NIR2-folate was applied at different time points after serum transfer, and the inflamed joints could be detected as early as 30 hours after arthritogenic antibody transfer (1.8-fold increase in signal intensity). Fluorescence microscopy, histology, and immunohistochemistry validated the optical imaging results. We conclude that in vivo arthritis detection was feasible using a folate-targeted near-infrared fluorescence probe. This receptor-targeted imaging method may facilitate improved arthritis diagnosis and early assessment of the disease progress by providing an in vivo characterization of active macrophage status in inflammatory joint diseases. |
| Published Version: | doi: 10.1186/ar1483 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1065321/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:8160865 |
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