dc.contributor.author | Chen, Wei-Tsung | |
dc.contributor.author | Mahmood, Umar | |
dc.contributor.author | Weissleder, Ralph | |
dc.contributor.author | Tung, Ching-Hsuan | |
dc.date.accessioned | 2012-02-14T01:02:44Z | |
dc.date.issued | 2005 | |
dc.identifier.citation | Chen, Wei-Tsung, Umar Mahmood, Ralph Weissleder, and Ching-Hsuan Tung. 2005. Arthritis imaging using a near-infrared fluorescence folate-targeted probe. Arthritis Research & Therapy 7(2): R310-R317. | en_US |
dc.identifier.issn | 1478-6354 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:8160865 | |
dc.description.abstract | A recently developed near-infrared fluorescence-labeled folate probe (NIR2-folate) was tested for in vivo imaging of arthritis using a lipopolysaccharide intra-articular injection model and a KRN transgenic mice serum induction mouse model. In the lipopolysaccharide injection model, the fluorescence signal intensity of NIR2-folate (n = 12) and of free NIR2 (n = 5) was compared between lipopolysaccharide-treated and control joints. The fluorescence signal intensity of the NIR2-folate probe at the inflammatory joints was found to be significantly higher than the control normal joints (up to 2.3-fold, P < 0.001). The NIR2-free dye injection group showed a persistent lower enhancement ratio than the NIR2-folate probe injection group. Excessive folic acid was also given to demonstrate a competitive effect with the NIR2-folate. In the KRN serum transfer model (n = 4), NIR2-folate was applied at different time points after serum transfer, and the inflamed joints could be detected as early as 30 hours after arthritogenic antibody transfer (1.8-fold increase in signal intensity). Fluorescence microscopy, histology, and immunohistochemistry validated the optical imaging results. We conclude that in vivo arthritis detection was feasible using a folate-targeted near-infrared fluorescence probe. This receptor-targeted imaging method may facilitate improved arthritis diagnosis and early assessment of the disease progress by providing an in vivo characterization of active macrophage status in inflammatory joint diseases. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | BioMed Central | en_US |
dc.relation.isversionof | doi: 10.1186/ar1483 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1065321/pdf/ | en_US |
dash.license | LAA | |
dc.subject | arthritis | en_US |
dc.subject | fluorescence | en_US |
dc.subject | folate receptor | en_US |
dc.subject | folic acid | en_US |
dc.subject | near-infrared | en_US |
dc.subject | optical imaging | en_US |
dc.title | Arthritis Imaging Using a Near-Infrared Fluorescence Folate-Targeted Probe | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | Arthritis Research & Therapy | en_US |
dash.depositing.author | Weissleder, Ralph | |
dc.date.available | 2012-02-14T01:02:44Z | |
dash.affiliation.other | HMS^Radiology-Massachusetts General Hospital | en_US |
dash.affiliation.other | HMS^Systems Biology | en_US |
dc.identifier.doi | 10.1186/ar1483 | * |
dash.contributor.affiliated | Mahmood, Umar | |
dash.contributor.affiliated | Weissleder, Ralph | |