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dc.contributor.authorZhou, Chunshui
dc.contributor.authorSeibert, Volker
dc.contributor.authorGeyer, Rory
dc.contributor.authorRhee, Edward
dc.contributor.authorLyapina, Svetlana
dc.contributor.authorCope, Greg
dc.contributor.authorDeshaies, Raymond J
dc.contributor.authorWolf, Dieter A
dc.date.accessioned2012-02-14T01:08:52Z
dc.date.issued2001
dc.identifier.citationZhou, Chunshui, Volker Seibert, Rory Geyer, Edward Rhee, Svetlana Lyapina, Greg Cope, Raymond J. Deshaies, and Dieter A. Wolf. 2001. The fission yeast COP9/signalosome is involved in cullin modification by ubiquitin-related Ned8p. BMC Biochemistry 2: 7.en_US
dc.identifier.issn1471-2091en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:8160868
dc.description.abstractBackground: The function of the fission yeast cullins Pcu1p and Pcu4p requires modification by the ubiquitin-related peptide Ned8p. A recent report by Lyapina et al. shows that the COP9/signalosome (CSN), a multifunctional eight subunit complex, regulates Ned8p modification of Pcu1p. Disruption of caa1/csn1, which encodes subunit 1 of the putative S. pombe CSN, results in accumulation of Pcu1p exclusively in the modified form. However, it remained unclear whether this reflects global control of all cullins by the entire CSN complex. Results We demonstrate that multiple CSN subunits control Ned8p modification of Pcu3p, another fission yeast cullin, which, in complex with the RING domain protein Pip1p, forms a ubiquitin ligase that functions in cellular stress response. Pcu3p is modified by Ned8p on Lys 729 and accumulates exclusively in the neddylated form in cells lacking the CSN subunits 1, 3, 4, and 5. These CSN subunits co-elute with Pcu3p in gel filtration fractions corresponding to ~550 kDa and specifically bind both native and Ned8p-modified Pcu3p in vivo. While CSN does not influence the subcellular localization of Pcu3p, Pcu3p-associated in vitro ubiquitin ligase activity is stimulated in the absence of CSN. Conclusions: Taken together, our data suggest that CSN is a global regulator of Ned8p modification of multiple cullins and potentially other proteins involved in cellular regulation.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/1471-2091-2-7en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC37391/pdf/en_US
dash.licenseLAA
dc.titleThe Fission Yeast COP9/signalosome Is Involved in Cullin Modification by Ubiquitin-related Ned8pen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalBMC Biochemistryen_US
dash.depositing.authorRhee, Edward
dc.date.available2012-02-14T01:08:52Z
dash.affiliation.otherHMS^Anaesthesia-Massachusetts General Hospitalen_US
dc.identifier.doi10.1186/1471-2091-2-7*
dash.contributor.affiliatedRhee, Edward


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