Analysis of Mice Lacking DNaseI Hypersensitive Sites at the 5′ End of the IgH Locus
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Analysis of Mice Lacking DNaseI Hypersensitive Sites at the 5′ End of the IgH Locus
| Title: | Analysis of Mice Lacking DNaseI Hypersensitive Sites at the 5′ End of the IgH Locus |
| Author: |
Perlot, Thomas; Pawlitzky, Inka; Zarrin, Ali A.; Brodeur, Peter H.; Manis, John P.; Alt, Frederick W.
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Perlot, Thomas, Inka Pawlitzky, John P. Manis, Ali A. Zarrin, Peter H. Brodeur, and Frederick W. Alt. 2010. Analysis of Mice Lacking DNaseI Hypersensitive Sites at the 5' End of the IgH Locus. PLoS ONE 5(11): e13992. |
| Full Text & Related Files: |
2981565.pdf (3.405Mb; PDF) 
|
| Abstract: | The 5′ end of the IgH locus contains a cluster of DNaseI hypersensitive sites, one of which (HS1) was shown to be pro-B cell specific and to contain binding sites for the transcription factors PU.1, E2A, and Pax5. These data as well as the location of the hypersensitive sites at the 5′ border of the IgH locus suggested a possible regulatory function for these elements with respect to the IgH locus. To test this notion, we generated mice carrying targeted deletions of either the pro-B cell specific site HS1 or the whole cluster of DNaseI hypersensitive sites. Lymphocytes carrying these deletions appear to undergo normal development, and mutant B cells do not exhibit any obvious defects in V(D)J recombination, allelic exclusion, or class switch recombination. We conclude that deletion of these DNaseI hypersensitive sites does not have an obvious impact on the IgH locus or B cell development. |
| Published Version: | doi:10.1371/journal.pone.0013992 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2981565/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:8231691 |
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