PINK1 Is Selectively Stabilized on Impaired Mitochondria to Activate Parkin
DSpace/Manakin Repository
PINK1 Is Selectively Stabilized on Impaired Mitochondria to Activate Parkin
| Title: | PINK1 Is Selectively Stabilized on Impaired Mitochondria to Activate Parkin |
| Author: |
Narendra, Derek P.; Jin, Seok Min; Suen, Der-Fen; Cookson, Mark R.; Youle, Richard J.; Tanaka, Atsushi; Gautier, Clement A.; Shen, Jie
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Narendra, Derek P., Seok Min Jin, Atsushi Tanaka, Der-Fen Suen, Clement A. Gautier, Jie Shen, Mark R. Cookson, and Richard J. Youle. 2010. PINK1 Is selectively stabilized on impaired mitochondria to activate Parkin. PLoS Biology 8(1): e1000298. |
| Full Text & Related Files: |
2811155.pdf (8.023Mb; PDF) 
|
| Abstract: | Loss-of-function mutations in PINK1 and Parkin cause parkinsonism in humans and mitochondrial dysfunction in model organisms. Parkin is selectively recruited from the cytosol to damaged mitochondria to trigger their autophagy. How Parkin recognizes damaged mitochondria, however, is unknown. Here, we show that expression of PINK1 on individual mitochondria is regulated by voltage-dependent proteolysis to maintain low levels of PINK1 on healthy, polarized mitochondria, while facilitating the rapid accumulation of PINK1 on mitochondria that sustain damage. PINK1 accumulation on mitochondria is both necessary and sufficient for Parkin recruitment to mitochondria, and disease-causing mutations in PINK1 and Parkin disrupt Parkin recruitment and Parkin-induced mitophagy at distinct steps. These findings provide a biochemical explanation for the genetic epistasis between PINK1 and Parkin in Drosophila melanogaster. In addition, they support a novel model for the negative selection of damaged mitochondria, in which PINK1 signals mitochondrial dysfunction to Parkin, and Parkin promotes their elimination. |
| Published Version: | doi:10.1371/journal.pbio.1000298 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811155/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:8255738 |
This item appears in the following Collection(s)
-
HMS Scholarly Articles [3988]
Contact administrator regarding this item (to report mistakes or request changes)
