Actin Disassembly by Cofilin, Coronin, and Aip1 Occurs in Bursts and Is Inhibited by Barbed-end Cappers

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Actin Disassembly by Cofilin, Coronin, and Aip1 Occurs in Bursts and Is Inhibited by Barbed-end Cappers

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dc.contributor.author Kueh, Hao Yuan
dc.contributor.author Charras, Guillaume T.
dc.contributor.author Brieher, William M.
dc.contributor.author Mitchison, Timothy J.
dc.date.accessioned 2012-03-12T02:07:57Z
dc.date.issued 2008
dc.identifier.citation Kueh, Hao Yuan, Guillaume T. Charras, Timothy J. Mitchison, and William M. Brieher. 2008. Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and is inhibited by barbed-end cappers. The Journal of Cell Biology 182(2): 341-353. en_US
dc.identifier.issn 0021-9525 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:8347348
dc.description.abstract Turnover of actin filaments in cells requires rapid actin disassembly in a cytoplasmic environment that thermodynamically favors assembly because of high concentrations of polymerizable monomers. We here image the disassembly of single actin filaments by cofilin, coronin, and actin-interacting protein 1, a purified protein system that reconstitutes rapid, monomer-insensitive disassembly (Brieher, W.M., H.Y. Kueh, B.A. Ballif, and T.J. Mitchison. 2006. J. Cell Biol. 175:315–324). In this three-component system, filaments disassemble in abrupt bursts that initiate preferentially, but not exclusively, from both filament ends. Bursting disassembly generates unstable reaction intermediates with lowered affinity for CapZ at barbed ends. CapZ and cytochalasin D (CytoD), a barbed-end capping drug, strongly inhibit bursting disassembly. CytoD also inhibits actin disassembly in mammalian cells, whereas latrunculin B, a monomer sequestering drug, does not. We propose that bursts of disassembly arise from cooperative separation of the two filament strands near an end. The differential effects of drugs in cells argue for physiological relevance of this new disassembly pathway and potentially explain discordant results previously found with these drugs. en_US
dc.language.iso en_US en_US
dc.publisher The Rockefeller University Press en_US
dc.relation.isversionof doi:10.1083/jcb.200801027 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2483518/pdf/ en_US
dash.license LAA
dc.title Actin Disassembly by Cofilin, Coronin, and Aip1 Occurs in Bursts and Is Inhibited by Barbed-end Cappers en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal The Journal of Cell Biology en_US
dash.depositing.author Mitchison, Timothy J.
dc.date.available 2012-03-12T02:07:57Z
dash.affiliation.other HMS^Systems Biology en_US

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