Protection of Specific Maternal Messenger RNAs by the P Body Protein CGH-1 (Dhh1/RCK) During Caenorhabditis elegans Oogenesis

DSpace/Manakin Repository

Protection of Specific Maternal Messenger RNAs by the P Body Protein CGH-1 (Dhh1/RCK) During Caenorhabditis elegans Oogenesis

Citable link to this page

. . . . . .

Title: Protection of Specific Maternal Messenger RNAs by the P Body Protein CGH-1 (Dhh1/RCK) During Caenorhabditis elegans Oogenesis
Author: Boag, Peter R.; Atalay, Arzu; Robida, Stacey; Reinke, Valerie; Blackwell, Thomas Keith

Note: Order does not necessarily reflect citation order of authors.

Citation: Boag, Peter R., Arzu Atalay, Stacey Robida, Valerie Reinke, and T. Keith Blackwell. 2008. Protection of specific maternal messenger RNAs by the P body protein CGH-1 (Dhh1/RCK) during Caenorhabditis elegans oogenesis. The Journal of Cell Biology 182(3): 543-557.
Full Text & Related Files:
Abstract: During oogenesis, numerous messenger RNAs (mRNAs) are maintained in a translationally silenced state. In eukaryotic cells, various translation inhibition and mRNA degradation mechanisms congregate in cytoplasmic processing bodies (P bodies). The P body protein Dhh1 inhibits translation and promotes decapping-mediated mRNA decay together with Pat1 in yeast, and has been implicated in mRNA storage in metazoan oocytes. Here, we have investigated in Caenorhabditis elegans whether Dhh1 and Pat1 generally function together, and how they influence mRNA sequestration during oogenesis. We show that in somatic tissues, the Dhh1 orthologue (CGH-1) forms Pat1 (patr-1)-dependent P bodies that are involved in mRNA decapping. In contrast, during oogenesis, CGH-1 forms patr-1–independent mRNA storage bodies. CGH-1 then associates with translational regulators and a specific set of maternal mRNAs, and prevents those mRNAs from being degraded. Our results identify somatic and germ cell CGH-1 functions that are distinguished by the involvement of PATR-1, and reveal that during oogenesis, numerous translationally regulated mRNAs are specifically protected by a CGH-1–dependent mechanism. [Supplemental Material Index: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2500139/bin/jcb.200801183_index.html]
Published Version: doi:10.1083/jcb.200801183
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2500139/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:8350335

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters