Cytoskeletal Mechanics of Proplatelet Maturation and Platelet Release

DSpace/Manakin Repository

Cytoskeletal Mechanics of Proplatelet Maturation and Platelet Release

Citable link to this page

. . . . . .

Title: Cytoskeletal Mechanics of Proplatelet Maturation and Platelet Release
Author: Montalvo, Alejandro; Patel-Hett, Sunita; Devine, Matthew T.; Richardson, Jennifer L.; Larson, Mark K.; Thon, Jonathan Noah; Ehrlicher, Allen Joseph; Hoffmeister, Karin Maria; Hartwig, John Henry; Italiano, Joseph E.

Note: Order does not necessarily reflect citation order of authors.

Citation: Thon, Jonathan N., Alejandro Montalvo, Sunita Patel-Hett, Matthew T. Devine, Jennifer L. Richardson, Allen Ehrlicher, Mark K. Larson, Karin Hoffmeister, John H. Hartwig, and Joseph E. Italiano. 2010. Cytoskeletal mechanics of proplatelet maturation and platelet release. Journal of Cell Biology 191(4): 861-874.
Full Text & Related Files:
Abstract: Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of proplatelet elongation have been studied, the terminal steps of proplatelet maturation and platelet release remain poorly understood. To elucidate this process, released proplatelets were isolated, and their conversion into individual platelets was assessed. This enabled us to (a) define and quantify the different stages in platelet maturation, (b) identify a new intermediate stage in platelet production, the preplatelet, (c) delineate the cytoskeletal mechanics involved in preplatelet/proplatelet interconversion, and (d) model proplatelet fission and platelet release. Preplatelets are anucleate discoid particles 2–10 \(\mu\)m across that have the capacity to convert reversibly into elongated proplatelets by twisting microtubule-based forces that can be visualized in proplatelets expressing GFP–\(\beta\)1-tubulin. The release of platelets from the ends of proplatelets occurs at an increasing rate in time during culture, as larger proplatelets undergo successive fission, and is potentiated by shear.
Published Version: doi:10.1083/jcb.201006102
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2983072/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:8362484

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters