Targeted Next-Generation Sequencing of a Cancer Transcriptome Enhances Detection of Sequence Variants and Novel Fusion Transcripts

Targeted Next-Generation Sequencing of a Cancer Transcriptome Enhances Detection of Sequence Variants and Novel Fusion Transcripts

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Targeted Next-Generation Sequencing of a Cancer Transcriptome Enhances Detection of Sequence Variants and Novel Fusion Transcripts

dc.contributor.author	Levin, Joshua Z
dc.contributor.author	Berger, Michael F
dc.contributor.author	Adiconis, Xian
dc.contributor.author	Rogov, Peter
dc.contributor.author	Melnikov, Alexandre
dc.contributor.author	Fennell, Timothy
dc.contributor.author	Nusbaum, Chad
dc.contributor.author	Gnirke, Andreas
dc.contributor.author	Garraway, Levi Alexander
dc.date.accessioned	2012-04-08T03:15:21Z
dc.date.issued	2009
dc.identifier.citation	Levin, Joshua Z., Michael F. Berger, Xian Adiconis, Peter Rogov, Alexandre Melnikov, Timothy Fennell, Chad Nusbaum, Levi A. Garraway, and Andreas Gnirke. 2009. Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts. Genome Biology 10(10): R115.	en_US
dc.identifier.issn	1465-6906	en_US
dc.identifier.uri	http://nrs.harvard.edu/urn-3:HUL.InstRepos:8519632
dc.description.abstract	Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes.	en_US
dc.language.iso	en_US	en_US
dc.publisher	BioMed Central	en_US
dc.relation.isversionof	doi:10.1186/gb-2009-10-10-r115	en_US
dc.relation.hasversion	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2784330/pdf/	en_US
dash.license	LAA
dc.title	Targeted Next-Generation Sequencing of a Cancer Transcriptome Enhances Detection of Sequence Variants and Novel Fusion Transcripts	en_US
dc.type	Journal Article	en_US
dc.description.version	Version of Record	en_US
dc.relation.journal	Genome Biology	en_US
dash.depositing.author	Garraway, Levi Alexander
dc.date.available	2012-04-08T03:15:21Z
dash.affiliation.other	HMS^Medicine-Brigham and Women's Hospital	en_US