Targeted Next-Generation Sequencing of a Cancer Transcriptome Enhances Detection of Sequence Variants and Novel Fusion Transcripts

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Targeted Next-Generation Sequencing of a Cancer Transcriptome Enhances Detection of Sequence Variants and Novel Fusion Transcripts

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dc.contributor.author Levin, Joshua Z
dc.contributor.author Berger, Michael F
dc.contributor.author Adiconis, Xian
dc.contributor.author Rogov, Peter
dc.contributor.author Melnikov, Alexandre
dc.contributor.author Fennell, Timothy
dc.contributor.author Nusbaum, Chad
dc.contributor.author Gnirke, Andreas
dc.contributor.author Garraway, Levi Alexander
dc.date.accessioned 2012-04-08T03:15:21Z
dc.date.issued 2009
dc.identifier.citation Levin, Joshua Z., Michael F. Berger, Xian Adiconis, Peter Rogov, Alexandre Melnikov, Timothy Fennell, Chad Nusbaum, Levi A. Garraway, and Andreas Gnirke. 2009. Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts. Genome Biology 10(10): R115. en_US
dc.identifier.issn 1465-6906 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:8519632
dc.description.abstract Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/gb-2009-10-10-r115 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2784330/pdf/ en_US
dash.license LAA
dc.title Targeted Next-Generation Sequencing of a Cancer Transcriptome Enhances Detection of Sequence Variants and Novel Fusion Transcripts en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Genome Biology en_US
dash.depositing.author Garraway, Levi Alexander
dc.date.available 2012-04-08T03:15:21Z
dash.affiliation.other HMS^Medicine-Brigham and Women's Hospital en_US

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