Identification of Small RNAs in Francisella tularensis

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Identification of Small RNAs in Francisella tularensis

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dc.contributor.author Postic, Guillaume
dc.contributor.author Frapy, Eric
dc.contributor.author Dupuis, Marion
dc.contributor.author Dubail, Iharilalao
dc.contributor.author Charbit, Alain
dc.contributor.author Meibom, Karin L
dc.contributor.author Livny, Jonathan
dc.date.accessioned 2012-04-08T03:48:34Z
dc.date.issued 2010
dc.identifier.citation Postic, Guillaume, Eric Frapy, Marion Dupuis, Iharilalao Dubail, Jonathan Livny, Alain Charbit, and Karin L. Meibom. 2010. Identification of small RNAs in Francisella tularensis. BMC Genomics 11:625. en_US
dc.identifier.issn 1471-2164 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:8519635
dc.description.abstract Background: Regulation of bacterial gene expression by small RNAs (sRNAs) have proved to be important for many biological processes. Francisella tularensis is a highly pathogenic Gram-negative bacterium that causes the disease tularaemia in humans and animals. Relatively little is known about the regulatory networks existing in this organism that allows it to survive in a wide array of environments and no sRNA regulators have been identified so far. Results: We have used a combination of experimental assays and in silico prediction to identify sRNAs in F. tularensis strain LVS. Using a cDNA cloning and sequencing approach we have shown that F. tularensis expresses homologues of several sRNAs that are well-conserved among diverse bacteria. We have also discovered two abundant putative sRNAs that share no sequence similarity or conserved genomic context with any previously annotated regulatory transcripts. Deletion of either of these two loci led to significant changes in the expression of several mRNAs that likely include the cognate target(s) of these sRNAs. Deletion of these sRNAs did not, however, significantly alter F. tularensis growth under various stress conditions in vitro, its replication in murine cells, or its ability to induce disease in a mouse model of F. tularensis infection. We also conducted a genome-wide in silico search for intergenic loci that suggests F. tularensis encodes several other sRNAs in addition to the sRNAs found in our experimental screen. Conclusion: Our findings suggest that F. tularensis encodes a significant number of non-coding regulatory RNAs, including members of well conserved families of structural and housekeeping RNAs and other poorly conserved transcripts that may have evolved more recently to help F. tularensis deal with the unique and diverse set of environments with which it must contend. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/1471-2164-11-625 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091763/pdf/ en_US
dash.license LAA
dc.title Identification of Small RNAs in Francisella tularensis en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BMC Genomics en_US
dash.depositing.author Livny, Jonathan
dc.date.available 2012-04-08T03:48:34Z
dash.affiliation.other HMS^Medicine-Brigham and Women's Hospital en_US

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