Roles of Major Facilitator Superfamily Transporters in Phosphate Response in \(Drosophila\)

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Roles of Major Facilitator Superfamily Transporters in Phosphate Response in \(Drosophila\)

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dc.contributor.author Rasmussen, Matthew D.
dc.contributor.author DeRobertis, Charles
dc.contributor.author Wee, Mark J.
dc.contributor.author Sinha, Sumi
dc.contributor.author Chen, Hway H.
dc.contributor.author Huang, Joanne
dc.contributor.author Bergwitz, Clemens
dc.contributor.author Perrimon, Norbert
dc.date.accessioned 2012-04-25T17:58:39Z
dc.date.issued 2012
dc.identifier.citation Bergwitz, Clemens, Matthew D. Rasmussen, Charles DeRobertis, Mark J. Wee, Sumi Sinha, Hway H. Chen, Joanne Huang, and Norbert Perrimon. 2012. Roles of major facilitator superfamily transporters in phosphate response in \(Drosophila\). PLoS ONE 7(2): e31730. en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:8620351
dc.description.abstract The major facilitator superfamily (MFS) transporter \(Pho84\) and the type III transporter \(Pho84\) are responsible for metabolic effects of inorganic phosphate in yeast. While the \(Pho84\) ortholog Pit1 was also shown to be involved in phosphate-activated MAPK in mammalian cells, it is currently unknown, whether orthologs of \(Pho84\) have a role in phosphate-sensing in metazoan species. We show here that the activation of MAPK by phosphate observed in mammals is conserved in \(Drosophila\) cells, and used this assay to characterize the roles of putative phosphate transporters. Surprisingly, while we found that RNAi-mediated knockdown of the fly \(Pho84\) ortholog dPit had little effect on the activation of MAPK in \(Drosophila\) S2R+ cells by phosphate, two \(Pho84\)/SLC17A1–9 MFS orthologs (MFS10 and MFS13) specifically inhibited this response. Further, using a \(Xenopus\) oocyte assay, we show that MSF13 mediates uptake of [33P]-orthophosphate in a sodium-dependent fashion. Consistent with a role in phosphate physiology, MSF13 is expressed highest in the \(Drosophila\) crop, midgut, Malpighian tubule, and hindgut. Altogether, our findings provide the first evidence that \(Pho84\) orthologs mediate cellular effects of phosphate in metazoan cells. Finally, while phosphate is essential for \(Drosophila\) larval development, loss of MFS13 activity is compatible with viability indicating redundancy at the levels of the transporters. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi:10.1371/journal.pone.0031730 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280997/pdf/ en_US
dash.license LAA
dc.subject biology en_US
dc.subject biochemistry en_US
dc.subject proteins en_US
dc.subject developmental biology en_US
dc.subject evolutionary biology en_US
dc.subject model organisms en_US
dc.subject animal models en_US
dc.subject molecular cell biology en_US
dc.subject signal transduction en_US
dc.subject signaling cascades en_US
dc.title Roles of Major Facilitator Superfamily Transporters in Phosphate Response in \(Drosophila\) en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS ONE en_US
dash.depositing.author Bergwitz, Clemens
dc.date.available 2012-04-25T17:58:39Z

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