Kinetics of Proton Transport into Influenza Virions by the Viral M2 Channel

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Kinetics of Proton Transport into Influenza Virions by the Viral M2 Channel

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dc.contributor.author Rozendaal, Rutger
dc.contributor.author Popovic, Milos
dc.contributor.author van Oijen, Antoine M.
dc.contributor.author Ivanovic, Tijana
dc.contributor.author Floyd, Daniel Lee
dc.contributor.author Harrison, Stephen C.
dc.date.accessioned 2012-05-22T19:16:24Z
dc.date.issued 2012
dc.identifier.citation Ivanovic, Tijana, Rutger Rozendaal, Daniel L. Floyd, Milos Popovic, Antoine M. van Oijen, and Stephen C. Harrison. 2012. Kinetics of proton transport into influenza virions by the viral m2 channel. PLoS ONE 7(3): e31566. en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:8796676
dc.description.abstract M2 protein of influenza A viruses is a tetrameric transmembrane proton channel, which has essential functions both early and late in the virus infectious cycle. Previous studies of proton transport by M2 have been limited to measurements outside the context of the virus particle. We have developed an in vitro fluorescence-based assay to monitor internal acidification of individual virions triggered to undergo membrane fusion. We show that rimantadine, an inhibitor of M2 proton conductance, blocks the acidification-dependent dissipation of fluorescence from a pH-sensitive virus-content probe. Fusion-pore formation usually follows internal acidification but does not require it. The rate of internal virion acidification increases with external proton concentration and saturates with a \(pK_m\) of ~4.7. The rate of proton transport through a single, fully protonated M2 channel is approximately 100 to 400 protons per second. The saturating proton-concentration dependence and the low rate of internal virion acidification derived from authentic virions support a transporter model for the mechanism of proton transfer. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi:10.1371/journal.pone.0031566 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3295812/pdf/ en_US
dash.license LAA
dc.subject biology en_US
dc.subject biochemistry en_US
dc.subject proteins en_US
dc.subject biophysics en_US
dc.subject computational biology en_US
dc.subject macromolecular structure analysis en_US
dc.subject microbiology en_US
dc.subject virology en_US
dc.subject viral transmission and infection en_US
dc.subject medicine en_US
dc.subject infectious diseases en_US
dc.subject viral diseases en_US
dc.title Kinetics of Proton Transport into Influenza Virions by the Viral M2 Channel en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS ONE en_US
dash.depositing.author Ivanovic, Tijana
dc.date.available 2012-05-22T19:16:24Z

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