Origins and Consequences of Velocity Fluctuations during DNA Passage through a Nanopore

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Origins and Consequences of Velocity Fluctuations during DNA Passage through a Nanopore

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Title: Origins and Consequences of Velocity Fluctuations during DNA Passage through a Nanopore
Author: Bo, Lu; Albertorio, Fernando; Hoogerheide, David Paul; Golovchenko, Jene A.

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Citation: Bo, Lu, Fernando Albertorio, David P. Hoogerheide, and Jene A. Golovchenko. 2011. Origins and consequences of velocity fluctuations during DNA passage through a nanopore. Biophysical Journal 101(1): 70-79.
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Abstract: We describe experiments and modeling results that reveal and explain the distribution of times that identical double-stranded DNA (dsDNA) molecules take to pass through a voltage-biased solid-state nanopore. We show that the observed spread in this distribution is caused by viscous-drag-induced velocity fluctuations that are correlated with the initial conformation of nanopore-captured molecules. This contribution exceeds that due to diffusional Brownian motion during the passage. Nevertheless, and somewhat counterintuitively, the diffusional Brownian motion determines the fundamental limitations of rapid DNA strand sequencing with a nanopore. We model both diffusional and conformational fluctuations in a Langevin description. It accounts well for passage time variations for DNA molecules of different lengths, and predicts conditions required for low-error-rate nanopore-strand DNA sequencing with nanopores.
Published Version: doi:10.1016/j.bpj.2011.05.034
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:8835476

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  • FAS Scholarly Articles [6929]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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