Origins and Consequences of Velocity Fluctuations during DNA Passage through a Nanopore

DSpace/Manakin Repository

Origins and Consequences of Velocity Fluctuations during DNA Passage through a Nanopore

Show simple item record

dc.contributor.author Bo, Lu
dc.contributor.author Albertorio, Fernando
dc.contributor.author Hoogerheide, David Paul
dc.contributor.author Golovchenko, Jene A.
dc.date.accessioned 2012-06-05T16:40:51Z
dc.date.issued 2011
dc.identifier.citation Bo, Lu, Fernando Albertorio, David P. Hoogerheide, and Jene A. Golovchenko. 2011. Origins and consequences of velocity fluctuations during DNA passage through a nanopore. Biophysical Journal 101(1): 70-79. en_US
dc.identifier.issn 0006-3495 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:8835476
dc.description.abstract We describe experiments and modeling results that reveal and explain the distribution of times that identical double-stranded DNA (dsDNA) molecules take to pass through a voltage-biased solid-state nanopore. We show that the observed spread in this distribution is caused by viscous-drag-induced velocity fluctuations that are correlated with the initial conformation of nanopore-captured molecules. This contribution exceeds that due to diffusional Brownian motion during the passage. Nevertheless, and somewhat counterintuitively, the diffusional Brownian motion determines the fundamental limitations of rapid DNA strand sequencing with a nanopore. We model both diffusional and conformational fluctuations in a Langevin description. It accounts well for passage time variations for DNA molecules of different lengths, and predicts conditions required for low-error-rate nanopore-strand DNA sequencing with nanopores. en_US
dc.description.sponsorship Engineering and Applied Sciences en_US
dc.description.sponsorship Physics en_US
dc.language.iso en_US en_US
dc.publisher Biophysical Society en_US
dc.relation.isversionof doi:10.1016/j.bpj.2011.05.034 en_US
dash.license OAP
dc.title Origins and Consequences of Velocity Fluctuations during DNA Passage through a Nanopore en_US
dc.type Journal Article en_US
dc.description.version Accepted Manuscript en_US
dc.relation.journal Biophysical Journal en_US
dash.depositing.author Golovchenko, Jene A.
dc.date.available 2012-06-05T16:40:51Z

Files in this item

Files Size Format View
Lu Bo et al final version.pdf 5.908Mb PDF View/Open

This item appears in the following Collection(s)

  • FAS Scholarly Articles [7495]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University

Show simple item record

 
 

Search DASH


Advanced Search
 
 

Submitters