Novel Subtype-Specific Genes Identify Distinct Subpopulations of Callosal Projection Neurons

DSpace/Manakin Repository

Novel Subtype-Specific Genes Identify Distinct Subpopulations of Callosal Projection Neurons

Citable link to this page

. . . . . .

Title: Novel Subtype-Specific Genes Identify Distinct Subpopulations of Callosal Projection Neurons
Author: Molyneaux, Bradley John; Arlotta, Paola; Fame, Ryan Marie; MacDonald, Jessica Linn; MacQuarrie, Kyle L.; Macklis, Jeffrey Daniel

Note: Order does not necessarily reflect citation order of authors.

Citation: Molyneaux, Bradley J., Paola Arlotta, Ryann M. Fame, Jessica L. MacDonald, Kyle L. MacQuarrie, and Jeffrey D. Macklis. 2009. Novel subtype-specific genes identify distinct subpopulations of callosal projection neurons. The Journal of Neuroscience 29(39): 12343-12354.
Full Text & Related Files:
Abstract: Little is known about the molecular development and heterogeneity of callosal projection neurons (CPN), cortical commissural neurons that connect homotopic regions of the two cerebral hemispheres via the corpus callosum and that are critical for bilateral integration of cortical information. Here we report on the identification of a series of genes that individually and in combination define CPN and novel CPN subpopulations during embryonic and postnatal development. We used in situ hybridization analysis, immunocytochemistry, and retrograde labeling to define the layer-specific and neuron-type-specific distribution of these newly identified CPN genes across different stages of maturation. We demonstrate that a subset of these genes (e.g., Hspb3 and Lpl) appear specific to all CPN (in layers II/III and V–VI), whereas others (e.g., Nectin-3, Plexin-D1, and Dkk3) discriminate between CPN of the deep layers and those of the upper layers. Furthermore, the data show that several genes finely subdivide CPN within individual layers and appear to label CPN subpopulations that have not been described previously using anatomical or morphological criteria. The genes identified here likely reflect the existence of distinct programs of gene expression governing the development, maturation, and function of the newly identified subpopulations of CPN. Together, these data define the first set of genes that identify and molecularly subcategorize distinct populations of callosal projection neurons, often located in distinct subdivisions of the canonical cortical laminae.
Published Version: doi:10.1523/JNEUROSCI.6108-08.2009
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2776075/
http://hscrb.unix.fas.harvard.edu/sites/default/files/Macklis_Molyneaux_Journal%20of%20Neuroscience_2009_09_30.pdf
http://www.hscrb.harvard.edu/sites/default/files/Macklis_Molyneaux_Journal%20of%20Neuroscience_2009_09_30.pdf
http://www.scrb.harvard.edu/sites/default/files/Macklis_Molyneaux_Journal%20of%20Neuroscience_2009_09_30.pdf
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:9637867

Show full Dublin Core record

This item appears in the following Collection(s)

  • FAS Scholarly Articles [7470]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

Search DASH


Advanced Search
 
 

Submitters