Early Life Nutrition Modulates Muscle Stem Cell Number: Implications for Muscle Mass and Repair

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Early Life Nutrition Modulates Muscle Stem Cell Number: Implications for Muscle Mass and Repair

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dc.contributor.author Woo, Melissa
dc.contributor.author Isganaitis, Elvira
dc.contributor.author Cerletti, Massimiliano
dc.contributor.author Fitzpatrick, Connor
dc.contributor.author Wagers, Amy Jo
dc.contributor.author Jimenez-Chillaron, Jose
dc.contributor.author Patti, Mary-Elizabeth
dc.date.accessioned 2012-09-26T12:34:58Z
dc.date.issued 2011
dc.identifier.citation Woo, Melissa, Elvira Isganaitis, Massimiliano Cerletti, Connor Fitzpatrick, Amy J. Wagers, Jose Jimenez-Chillaron, and Mary Elizabeth Patti. 2011. Early life nutrition modulates muscle stem cell number: implications for muscle mass and repair. Stem Cells and Development 20(10): 1763-1769. en_US
dc.identifier.issn 1547-3287 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:9639970
dc.description.abstract Suboptimal nutrition during prenatal and early postnatal development is associated with increased risk for type 2 diabetes during adult life. A hallmark of such diabetes risk is altered body composition, including reduced lean mass and increased adiposity. Since stem cell number and activity are important determinants of muscle mass, modulation of perinatal nutrition could alter stem cell number/function, potentially mediating developmentally programmed reductions in muscle mass. Skeletal muscle precursors (SMP) were purified from muscle of mice subjected to prenatal undernutrition and/or early postnatal high-fat diet (HFD)—experimental models that are both associated with obesity and diabetes risk. SMP number was determined by flow cytometry, proliferative capacity measured in vitro, and regenerative capacity of these cells determined in vivo after muscle freeze injury. Prenatally undernutrition (UN) mice showed significantly reduced SMP frequencies [Control (C) \(4.8\%\pm0.3\%\) (% live cells) vs. UN \(3.2\%\pm0.4\%, P = 0.015\)] at 6 weeks; proliferative capacity was unaltered. Reduced SMP in UN was associated with 32% decrease in regeneration after injury (\(C 16\%\pm3\%\) of injured area vs. \(UN 11\%\pm2\%; P < 0.0001\)). SMP frequency was also reduced in HFD-fed mice (chow \(6.4\%\pm0.6\% vs. HFD 4.7\% \pm0.4\%, P = 0.03\)), and associated with \(44\%\) decreased regeneration (chow \(16\%\pm2.7\% vs. HFD 9\%\pm2.2\%; P < 0.0001\)). Prenatal undernutrition was additive with postnatal HFD. Thus, both prenatal undernutrition and postnatal overnutrition reduce myogenic stem cell frequency and function, indicating that developmentally established differences in muscle-resident stem cell populations may provoke reductions in muscle mass and repair and contribute to diabetes risk. en_US
dc.description.sponsorship Stem Cell and Regenerative Biology en_US
dc.language.iso en_US en_US
dc.publisher Mary Ann Liebert en_US
dc.relation.isversionof DOI: 10.1089/scd.2010.0349 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pubmed/21247245 en_US
dash.license LAA
dc.title Early Life Nutrition Modulates Muscle Stem Cell Number: Implications for Muscle Mass and Repair en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Stem Cells and Development en_US
dash.depositing.author Wagers, Amy Jo
dc.date.available 2012-09-26T12:34:58Z

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  • FAS Scholarly Articles [7213]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University

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