Genomic Analysis of the Hydrocarbon-Producing, Cellulolytic, Endophytic Fungus Ascocoryne sarcoides

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Genomic Analysis of the Hydrocarbon-Producing, Cellulolytic, Endophytic Fungus Ascocoryne sarcoides

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dc.contributor.author Sboner, Andrea
dc.contributor.author Kodira, Chinnappa
dc.contributor.author Egholm, Michael
dc.contributor.author Church, George McDonald
dc.contributor.author Gianoulis, Tara A.
dc.contributor.author Griffin, Meghan A.
dc.contributor.author Spakowicz, Daniel J.
dc.contributor.author Dunican, Brian F.
dc.contributor.author Alpha, Cambria J.
dc.contributor.author Sismour, A. Michael
dc.contributor.author Gerstein, Mark B.
dc.contributor.author Strobel, Scott A.
dc.date.accessioned 2012-10-09T01:27:44Z
dc.date.issued 2012
dc.identifier.citation Gianoulis, Tara A., Meghan A. Griffin, Daniel J. Spakowicz, Brian F. Dunican, Cambria J. Alpha, Andrea Sboner, A. Michael Sismour, et al. 2012. Genomic analysis of the hydrocarbon-producing, cellulolytic, endophytic fungus Ascocoryne sarcoides. PLoS Genetics 8(3): e1002558. en_US
dc.identifier.issn 1553-7390 en_US
dc.identifier.issn 1553-7404 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:9696331
dc.description.abstract The microbial conversion of solid cellulosic biomass to liquid biofuels may provide a renewable energy source for transportation fuels. Endophytes represent a promising group of organisms, as they are a mostly untapped reservoir of metabolic diversity. They are often able to degrade cellulose, and they can produce an extraordinary diversity of metabolites. The filamentous fungal endophyte Ascocoryne sarcoides was shown to produce potential-biofuel metabolites when grown on a cellulose-based medium; however, the genetic pathways needed for this production are unknown and the lack of genetic tools makes traditional reverse genetics difficult. We present the genomic characterization of A. sarcoides and use transcriptomic and metabolomic data to describe the genes involved in cellulose degradation and to provide hypotheses for the biofuel production pathways. In total, almost 80 biosynthetic clusters were identified, including several previously found only in plants. Additionally, many transcriptionally active regions outside of genes showed condition-specific expression, offering more evidence for the role of long non-coding RNA in gene regulation. This is one of the highest quality fungal genomes and, to our knowledge, the only thoroughly annotated and transcriptionally profiled fungal endophyte genome currently available. The analyses and datasets contribute to the study of cellulose degradation and biofuel production and provide the genomic foundation for the study of a model endophyte system. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi:10.1371/journal.pgen.1002558 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291568/pdf/ en_US
dash.license LAA
dc.title Genomic Analysis of the Hydrocarbon-Producing, Cellulolytic, Endophytic Fungus Ascocoryne sarcoides en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS Genetics en_US
dash.depositing.author Church, George McDonald
dc.date.available 2012-10-09T01:27:44Z

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