Mapping Molecular Agents Distributions in Whole Mice Hearts Using Born-Normalized Optical Projection Tomography

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Mapping Molecular Agents Distributions in Whole Mice Hearts Using Born-Normalized Optical Projection Tomography

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dc.contributor.author Feruglio, Paolo Fumene
dc.contributor.author Razansky, Daniel
dc.contributor.author Gorbatov, Rostic
dc.contributor.author Ntziachristos, Vasilis
dc.contributor.author Sbarbati, Andrea
dc.contributor.author Vinegoni, Claudio
dc.contributor.author Nahrendorf, Matthias
dc.contributor.author Weissleder, Ralph
dc.date.accessioned 2012-10-26T16:50:12Z
dc.date.issued 2012
dc.identifier.citation Vinegoni, Claudio, Paolo Fumene Feruglio, Daniel Razansky, Rostic Gorbatov, Vasilis Ntziachristos, Andrea Sbarbati, Matthias Nahrendorf, and Ralph Weissleder. 2012. Mapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomography. PLoS ONE 7(4): e34427. en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:9809494
dc.description.abstract To date there is a lack of tools to map the spatio-temporal dynamics of diverse cells in experimental heart models. Conventional histology is labor intensive with limited coverage, whereas many imaging techniques do not have sufficiently high enough spatial resolution to map cell distributions. We have designed and built a high resolution, dual channel Born-normalized near-infrared fluorescence optical projection tomography system to quantitatively and spatially resolve molecular agents distribution within whole murine heart. We validated the use of the system in a mouse model of monocytes/macrophages recruitment during myocardial infarction. While acquired, data were processed and reconstructed in real time. Tomographic analysis and visualization of the key inflammatory components were obtained via a mathematical formalism based on left ventricular modeling. We observed extensive monocyte recruitment within and around the infarcted areas and discovered that monocytes were also extensively recruited into non-ischemic myocardium, beyond that of injured tissue, such as the septum. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi:10.1371/journal.pone.0034427 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3324534/pdf/ en_US
dash.license LAA
dc.subject biology en_US
dc.subject developmental biology en_US
dc.subject morphogenesis en_US
dc.subject heart development en_US
dc.subject model organisms en_US
dc.subject animal models en_US
dc.subject anatomy and physiology en_US
dc.subject cardiovascular system en_US
dc.subject cardiovascular anatomy en_US
dc.subject cardiovascular imaging en_US
dc.subject heart failure en_US
dc.subject radiology en_US
dc.title Mapping Molecular Agents Distributions in Whole Mice Hearts Using Born-Normalized Optical Projection Tomography en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS ONE en_US
dash.depositing.author Vinegoni, Claudio
dc.date.available 2012-10-26T16:50:12Z

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