Pin-Hole Array Correlation Imaging: Highly Parallel Fluorescence Correlation Spectroscopy

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Pin-Hole Array Correlation Imaging: Highly Parallel Fluorescence Correlation Spectroscopy

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Title: Pin-Hole Array Correlation Imaging: Highly Parallel Fluorescence Correlation Spectroscopy
Author: Needleman, Daniel Joseph; Xu, Yangqing; Mitchison, Timothy J.

Note: Order does not necessarily reflect citation order of authors.

Citation: Needleman, Daniel J., Yangqing Xu, and Timothy J. Mitchison. 2009. Pin-hole array correlation imaging: Highly parallel fluorescence correlation spectroscopy. Biophysical Journal 96(12): 5050-5059.
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Abstract: In this work, we describe pin-hole array correlation imaging, a multipoint version of fluorescence correlation spectroscopy, based upon a stationary Nipkow disk and a high-speed electron multiplying charged coupled detector. We characterize the system and test its performance on a variety of samples, including 40 nm colloids, a fluorescent protein complex, a membrane dye, and a fluorescence fusion protein. Our results demonstrate that pin-hole array correlation imaging is capable of simultaneously performing tens or hundreds of fluorescence correlation spectroscopy-style measurements in cells, with sufficient sensitivity and temporal resolution to study the behaviors of membrane-bound and soluble molecules labeled with conventional chemical dyes or fluorescent proteins.
Published Version: doi:10.1016/j.bpj.2009.03.023
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:9949288

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  • FAS Scholarly Articles [6466]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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