dc.contributor.author | Needleman, Daniel Joseph | |
dc.contributor.author | Xu, Yangqing | |
dc.contributor.author | Mitchison, Timothy J. | |
dc.date.accessioned | 2012-11-27T21:35:27Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Needleman, Daniel J., Yangqing Xu, and Timothy J. Mitchison. 2009. Pin-hole array correlation imaging: Highly parallel fluorescence correlation spectroscopy. Biophysical Journal 96(12): 5050-5059. | en_US |
dc.identifier.issn | 0006-3495 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:9949288 | |
dc.description.abstract | In this work, we describe pin-hole array correlation imaging, a multipoint version of fluorescence correlation spectroscopy, based upon a stationary Nipkow disk and a high-speed electron multiplying charged coupled detector. We characterize the system and test its performance on a variety of samples, including 40 nm colloids, a fluorescent protein complex, a membrane dye, and a fluorescence fusion protein. Our results demonstrate that pin-hole array correlation imaging is capable of simultaneously performing tens or hundreds of fluorescence correlation spectroscopy-style measurements in cells, with sufficient sensitivity and temporal resolution to study the behaviors of membrane-bound and soluble molecules labeled with conventional chemical dyes or fluorescent proteins. | en_US |
dc.description.sponsorship | Engineering and Applied Sciences | en_US |
dc.description.sponsorship | Molecular and Cellular Biology | en_US |
dc.description.sponsorship | Other Research Unit | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | doi:10.1016/j.bpj.2009.03.023 | en_US |
dash.license | LAA | |
dc.subject | fluorescence correlation spectroscopy | en_US |
dc.subject | spinning disk confocal | en_US |
dc.subject | image correlation spectroscopy | en_US |
dc.subject | electron multiplying charge coupled detector | en_US |
dc.subject | Nipkow disk | en_US |
dc.subject | FOXO | en_US |
dc.title | Pin-Hole Array Correlation Imaging: Highly Parallel Fluorescence Correlation
Spectroscopy | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Author's Original | en_US |
dc.relation.journal | Biophysical Journal | en_US |
dash.depositing.author | Needleman, Daniel Joseph | |
dc.date.available | 2012-11-27T21:35:27Z | |
dc.identifier.doi | 10.1016/j.bpj.2009.03.023 | * |
dash.contributor.affiliated | Xu, Yangqing | |
dash.contributor.affiliated | Needleman, Daniel | |
dash.contributor.affiliated | Mitchison, Timothy | |