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dc.contributor.authorNeedleman, Daniel Joseph
dc.contributor.authorXu, Yangqing
dc.contributor.authorMitchison, Timothy J.
dc.date.accessioned2012-11-27T21:35:27Z
dc.date.issued2009
dc.identifier.citationNeedleman, Daniel J., Yangqing Xu, and Timothy J. Mitchison. 2009. Pin-hole array correlation imaging: Highly parallel fluorescence correlation spectroscopy. Biophysical Journal 96(12): 5050-5059.en_US
dc.identifier.issn0006-3495en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:9949288
dc.description.abstractIn this work, we describe pin-hole array correlation imaging, a multipoint version of fluorescence correlation spectroscopy, based upon a stationary Nipkow disk and a high-speed electron multiplying charged coupled detector. We characterize the system and test its performance on a variety of samples, including 40 nm colloids, a fluorescent protein complex, a membrane dye, and a fluorescence fusion protein. Our results demonstrate that pin-hole array correlation imaging is capable of simultaneously performing tens or hundreds of fluorescence correlation spectroscopy-style measurements in cells, with sufficient sensitivity and temporal resolution to study the behaviors of membrane-bound and soluble molecules labeled with conventional chemical dyes or fluorescent proteins.en_US
dc.description.sponsorshipEngineering and Applied Sciencesen_US
dc.description.sponsorshipMolecular and Cellular Biologyen_US
dc.description.sponsorshipOther Research Uniten_US
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.relation.isversionofdoi:10.1016/j.bpj.2009.03.023en_US
dash.licenseLAA
dc.subjectfluorescence correlation spectroscopyen_US
dc.subjectspinning disk confocalen_US
dc.subjectimage correlation spectroscopyen_US
dc.subjectelectron multiplying charge coupled detectoren_US
dc.subjectNipkow disken_US
dc.subjectFOXOen_US
dc.titlePin-Hole Array Correlation Imaging: Highly Parallel Fluorescence Correlation Spectroscopyen_US
dc.typeJournal Articleen_US
dc.description.versionAuthor's Originalen_US
dc.relation.journalBiophysical Journalen_US
dash.depositing.authorNeedleman, Daniel Joseph
dc.date.available2012-11-27T21:35:27Z
dc.identifier.doi10.1016/j.bpj.2009.03.023*
dash.contributor.affiliatedXu, Yangqing
dash.contributor.affiliatedNeedleman, Daniel
dash.contributor.affiliatedMitchison, Timothy


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