Browsing by Author "Binari, Richard"

Now showing items 1-14 of 14

    • Combining Genetic Perturbations and Proteomics to Examine Kinase-Phosphatase Networks in Drosophila Embryos 

      Sopko, Richelle; Foos, Marianna; Vinayagam, Arunachalam; Zhai, Bo; Binari, Richard; Hu, Yanhui; Randklev, Sakara; Perkins, Lizabeth A.; Gygi, Steven; Perrimon, Norbert (Elsevier BV, 2014-10-13)
      Connecting phosphorylation events to kinases and phosphatases is key to understanding the molecular organization and signaling dynamics of networks. We have generated a validated set of transgenic RNA-interference reagents ...

    • Drosophila Unpaired Encodes a Secreted Protein That Activates the JAK Signaling Pathway 

      Harrison, Douglas A.; McCoon, Patricia E.; Binari, Richard; Gilman, Michael; Perrimon, Norbert (Cold Spring Harbor Laboratory, 1998-10-15)
      In vertebrates, many cytokines and growth factors have been identified as activators of the JAK/STAT signaling pathway. In Drosophila, JAK and STAT molecules have been isolated, but no ligands or receptors capable of ...

    • Genetic Odyssey to Generate Marked Clones in Drosophila Mosaics 

      Griffin, Ruth; Binari, Richard; Perrimon, Norbert (National Academy of Sciences, 2014-04-01)
      Chimaeras, fanciful beasts that drew their force from being composed of parts of disparate animals, have stimulated our collective imagination for centuries. In modern terms, chimaeras are composite animals consisting of ...

    • A Genome-Scale shRNA Resource for Transgenic RNAi in Drosophila 

      Ni, Jian-Quan; Zhou, Rui; Czech, Benjamin; Liu, Lu-Ping; Holderbaum, Laura; Yang-Zhou, Donghui; Shim, Hye-Seok; Tao, Rong; Handler, Dominik; Karpowicz, Phillip; Binari, Richard; Booker, Matthew; Brennecke, Julius; Perkins, Lizabeth A.; Hannon, Gregory J.; Perrimon, Norbert (Springer Science and Business Media LLC, 2011-05)
      Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important ...

    • Hierarchical Rules for Argonaute Loading in Drosophila 

      Czech, Benjamin; Zhou, Rui; Erlich, Yaniv; Brennecke, Julius; Binari, Richard; Villalta, Christians; Gordon, Assaf; Perrimon, Norbert; Hannon, Gregory J. (Elsevier BV, 2009-11)
      Drosophila Argonaute-1 and Argonaute-2 differ in function and small RNA content. AGO2 binds to siRNAs, whereas AGO1 is almost exclusively occupied by microRNAs. MicroRNA duplexes are intrinsically asymmetric, with one ...

    • PAPTi: A Peptide Aptamer Interference Toolkit for Perturbation of Protein-Protein Interaction Networks 

      Yeh, Johannes T. -H.; Binari, Richard; Gocha, Tenzin; Dasgupta, Ramanuj; Perrimon, Norbert (Springer Science and Business Media LLC, 2013-01-29)
      Signaling proteins often form dynamic protein-protein interaction (PPI) complexes to achieve multi-functionality. Methods to abrogate a subset of PPI interfaces without depleting the full-length protein will be valuable ...

    • Proteomic and Functional Genomic Landscape of Receptor Tyrosine Kinase and Ras to Extracellular Signal-Regulated Kinase Signaling 

      Friedman, Adam A.; Tucker, George; Singh, Rohit; Yan, Dong; Vinayagam, Arunachalam; Hu, Yanhui; Binari, Richard; Hong, Pengyu; Sun, Xiaoyun; Porto, Maura; Pacifico, Svetlana; Murali, Thilakam; Finley, Russell L.; Asara, John; Berger, Bonnie; Perrimon, Norbert (American Association for the Advancement of Science (AAAS), 2011-10-25)
      Characterizing the extent and logic of signaling networks is essential to understanding specificity in such physiological and pathophysiological contexts as cell fate decisions and mechanisms of oncogenesis and resistance ...

    • A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal 

      Yan, Dong; Neumüller, Ralph A.; Buckner, Michael; Ayers, Kathleen; Li, Hua; Hu, Yanhui; Yang-Zhou, Donghui; Pan, Lei; Wang, Xiaoxi; Kelley, Colleen; Vinayagam, Arunachalam; Binari, Richard; Randklev, Sakara; Perkins, Lizabeth A.; Xie, Ting; Cooley, Lynn; Perrimon, Norbert (Elsevier BV, 2014-02-24)
      Stem cells possess the capacity to generate two cells of distinct fate upon division; one cell retaining stem cell identity and the other cell destined to differentiate. These cell fates are established by cell-type-specific ...

    • RNA Interference Screening in Drosophila Primary Cells for Genes Involved in Muscle Assembly and Maintenance 

      Bai, Jianwu; Binari, Richard; Ni, Jian-Quan; Vijayakanthan, Marina; Li, Hong-Sheng; Perrimon, Norbert (The Company of Biologists, 2008-04-15)
      To facilitate the genetic analysis of muscle assembly and maintenance, we have developed a method for efficient RNA interference (RNAi) in Drosophila primary cells using double-stranded RNAs (dsRNAs). First, using molecular ...

    • Stripe-Specific Regulation of Pair-Rule Genes by Hopscotch, a Putative Jak Family Tyrosine Kinase in Drosophila. 

      Binari, Richard; Perrimon, Norbert (Cold Spring Harbor Laboratory, 1994-02-01)
      We describe the characterization of the Drosophila gene, hopscotch (hop), which is required maternally for the establishment of the normal array of embryonic segments. In hop embryos, although expression of the gap genes ...

    • A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs 

      Fulga, Tudor A.; McNeill, Elizabeth M.; Binari, Richard; Yelick, Julia; Blanche, Alexandra; Booker, Matthew; Steinkraus, Bruno R.; Schnall-Levin, Michael; Zhao, Yong; DeLuca, Todd; Bejarano, Fernando; Han, Zhe; Lai, Eric C.; Wall, Dennis P.; Perrimon, Norbert; Van Vactor, David (Nature Pub. Group, 2015)
      Although the impact of microRNAs (miRNAs) in development and disease is well established, understanding the function of individual miRNAs remains challenging. Development of competitive inhibitor molecules such as miRNA ...

    • The Twin Spot Generator for Differential Drosophila Lineage Analysis 

      Griffin, Ruth; Sustar, Anne; Bonvin, Marianne; Binari, Richard; del Valle Rodriguez, Alberto; Hohl, Amber M; Bateman, Jack R; Villalta, Christians; Heffern, Elleard; Grunwald, Didier; Bakal, Chris; Desplan, Claude; Schubiger, Gerold; Wu, C-ting; Perrimon, Norbert (Springer Science and Business Media LLC, 2009-07-26)
      In Drosophila, widely-used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the Twin ...

    • Vector and Parameters for Targeted Transgenic RNA Interference in Drosophila melanogaster 

      Ni, Jian-Quan; Markstein, Michele; Binari, Richard; Pfeiffer, Barret D.; Liu, Lu-Ping; Villalta, Christians; Booker, Matthew; Perkins, Lizabeth; Perrimon, Norbert (Springer Nature, 2008-01)
      The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site–driven RNA interference constructs ...

    • Wildtype Adult Stem Cells, Unlike Tumor Cells, Are Resistant to Cellular Damages in Drosophila 

      Ma, Meifang; Zhao, Hang; Zhao, Hanfei; Binari, Richard; Perrimon, Norbert; Li, Zhouhua (Elsevier BV, 2016-03-15)
      Adult stem cells or residential progenitor cells are critical to maintain the structure and function of adult tissues (homeostasis) throughout the lifetime of an individual. Mis-regulation of stem cell proliferation and ...