Browsing by Author "Clapham, David"
Now showing items 1-20 of 21
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Anion-Sensitive Fluorophore Identifies the Drosophila Swell-Activated Chloride Channel in a Genome-Wide RNA Interference Screen
Stotz, Stephanie Christine; Clapham, David Eldon (Public Library of Science, 2012)When cells swell in hypo-osmotic solutions, chloride-selective ion channels (\(Cl_{swell}\)) activate to reduce intracellular osmolality and prevent catastrophic cell rupture. Despite intensive efforts to assign a molecular ... -
Atypical calcium regulation of the PKD2-L1 polycystin ion channel
DeCaen, Paul G; Liu, Xiaowen; Abiria, Sunday; Clapham, David E (eLife Sciences Publications, Ltd, 2016)Native PKD2-L1 channel subunits are present in primary cilia and other restricted cellular spaces. Here we investigate the mechanism for the channel's unusual regulation by external calcium, and rationalize this behavior ... -
Caspase-11 Controls Interleukin-1β Release through Degradation of TRPC1
Py, Bénédicte F.; Jin, Mingzhi; Desai, Bimal N.; Penumaka, Anirudh; Zhu, Hong; Kober, Maike; Dietrich, Alexander; Lipinski, Marta M.; Henry, Thomas; Clapham, David E.; Yuan, Junying (2014)SUMMARY Caspase-11 is a highly inducible caspase that controls both inflammatory responses and cell death. Caspase-11 controls interleukin 1β (IL-1β) secretion by potentiating caspase-1 activation and induces caspase-1-independent ... -
Cellular Metabolism Modulates Ion Channels That Regulate Neuronal Excitability
Lutas, Andrew (2015-05-01)Epilepsy is a common neurological disorder, affecting around 1% of the world’s population. For many, drugs are available that can prevent their seizures. However, for close to one third of those who suffer from epilepsy, ... -
Characterization of human TRPA1 and TRPV1 channels in response to naturally occurring defensive compounds
Ibarra, Yessenia Michelle (2013-10-08)The transient receptor potential channels, ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1), are non-selective cation-permeable channels that have retained their function as chemical sensors since their first appearance in metazoan ... -
Controlled delivery of bioactive molecules into live cells using the bacterial mechanosensitive channel MscL
Doerner, Julia; Febvay, Sebastien; Clapham, David Eldon (2013)Bacterial mechanosensitive channels are some of the largest pores in nature. In particular, MscL, with a pore diameter > 25 Å, allows passage of large organic ions and small proteins. Functional MscL reconstitution into ... -
Direct recording and molecular identification of the calcium channel of primary cilia
DeCaen, Paul G.; Delling, Markus; Vien, Thuy N.; Clapham, David E. (2014)Summary A primary cilium is a solitary slender non-motile protuberance of structured microtubules (9+0) enclosed by plasma membrane1. Housing components of the cell division apparatus between cell divisions, they also serve ... -
Evolutionary Genomics Reveals Lineage-Specific Gene Loss and Rapid Evolution of a Sperm-Specific Ion Channel Complex: CatSpers and CatSperβ
Cai, Xinjiang; Clapham, David Eldon; Schwartz, Arnold (Public Library of Science, 2008)The mammalian CatSper ion channel family consists of four sperm-specific voltage-gated Ca2+ channels that are crucial for sperm hyperactivation and male fertility. All four CatSper subunits are believed to assemble into a ... -
Functional reconstitution of the mitochondrial Ca2+/H+ antiporter Letm1
Tsai, Ming-Feng; Jiang, Dawei; Zhao, Linlin; Clapham, David; Miller, Christopher (The Rockefeller University Press, 2014)The leucine zipper, EF hand–containing transmembrane protein 1 (Letm1) gene encodes a mitochondrial inner membrane protein, whose depletion severely perturbs mitochondrial Ca2+ and K+ homeostasis. Here we expressed, purified, ... -
The G-protein–gated K+ channel, IKACh, is required for regulation of pacemaker activity and recovery of resting heart rate after sympathetic stimulation
Mesirca, Pietro; Marger, Laurine; Toyoda, Futoshi; Rizzetto, Riccardo; Audoubert, Matthieu; Dubel, Stefan; Torrente, Angelo G.; DiFrancesco, Mattia L.; Muller, Jana Christina; Leoni, Anne-Laure; Couette, Brigitte; Nargeot, Joël; Clapham, David E.; Wickman, Kevin; Mangoni, Matteo E. (The Rockefeller University Press, 2013)Parasympathetic regulation of sinoatrial node (SAN) pacemaker activity modulates multiple ion channels to temper heart rate. The functional role of the G-protein–activated K+ current (IKACh) in the control of SAN pacemaking ... -
Ion channels and calcium signaling in motile cilia
Doerner, Julia F; Delling, Markus; Clapham, David E (eLife Sciences Publications, Ltd, 2015)The beating of motile cilia generates fluid flow over epithelia in brain ventricles, airways, and Fallopian tubes. Here, we patch clamp single motile cilia of mammalian ependymal cells and examine their potential function ... -
Ionic selectivity and thermal adaptations within the voltage-gated sodium channel family of alkaliphilic Bacillus
DeCaen, Paul G; Takahashi, Yuka; Krulwich, Terry A; Ito, Masahiro; Clapham, David E (eLife Sciences Publications, Ltd, 2014)Entry and extrusion of cations are essential processes in living cells. In alkaliphilic prokaryotes, high external pH activates voltage-gated sodium channels (Nav), which allows Na+ to enter and be used as substrate for ... -
MCU encodes the pore conducting mitochondrial calcium currents
Chaudhuri, Dipayan; Sancak, Yasemin; Mootha, Vamsi K; Clapham, David E (eLife Sciences Publications, Ltd, 2013)Mitochondrial calcium (Ca2+) import is a well-described phenomenon regulating cell survival and ATP production. Of multiple pathways allowing such entry, the mitochondrial Ca2+ uniporter is a highly Ca2+-selective channel ... -
Molecular basis of ion permeability in a voltage‐gated sodium channel
Naylor, Claire E; Bagnéris, Claire; DeCaen, Paul G; Sula, Altin; Scaglione, Antonella; Clapham, David E; Wallace, BA (John Wiley and Sons Inc., 2016)Abstract Voltage‐gated sodium channels are essential for electrical signalling across cell membranes. They exhibit strong selectivities for sodium ions over other cations, enabling the finely tuned cascade of events ... -
The mother of all endocytosis
Clapham, David E (eLife Sciences Publications, Ltd, 2013)Massive endocytosis is initiated by a series of steps that involve a sudden influx of calcium ions, changes in mitochondria, and modification of surface proteins by lipids. A better understanding of this process could lead ... -
A Novel Gene Required for Male Fertility and Functional CATSPER Channel Formation in Spermatozoa
Chung, Jean-Ju; Navarro, Betsy; Krapivinsky, Grigory; Krapivinsky, Luba; Clapham, David E. (2014)Summary Calcium signaling is critical for successful fertilization. In spermatozoa, capacitation, hyperactivation of motility, and the acrosome reaction are all mediated by increases in intracellular Ca2+. Cation channels ... -
Primary cilia are specialized calcium signaling organelles
Delling, Markus; DeCaen, Paul G.; Doerner, Julia F.; Febvay, Sebastien; Clapham, David E. (2014)Summary Primary cilia are solitary nonmotile extensions of the centriole found on nearly all nucleated eukaryotic cells between cell divisions. Only ∼200-300 nm in diameter and a few microns long, they are separated from ... -
Role of the C-terminal domain in the structure and function of tetrameric sodium channels
Bagnéris, Claire; DeCaen, Paul G.; Hall, Benjamin A.; Naylor, Claire E.; Clapham, David E.; Kay, Christopher W. M.; Wallace, B. A. (Nature Pub. Group, 2013)Voltage-gated sodium channels have essential roles in electrical signalling. Prokaryotic sodium channels are tetramers consisting of transmembrane (TM) voltage-sensing and pore domains, and a cytoplasmic carboxy-terminal ... -
Simultaneous knockout of Slo3 and CatSper1 abolishes all alkalization- and voltage-activated current in mouse spermatozoa
Zeng, Xu-Hui; Navarro, Betsy; Xia, Xiao-Ming; Clapham, David E.; Lingle, Christopher J. (The Rockefeller University Press, 2013)During passage through the female reproductive tract, mammalian sperm undergo a maturation process termed capacitation that renders sperm competent to produce fertilization. Capacitation involves a sequence of changes in ... -
Sperm BerserKers
Clapham, David E (eLife Sciences Publications, Ltd, 2013)Human sperm cells rely on an unusual type of potassium ion channel.