Now showing items 1-20 of 29

    • 3D Multicolor Super-Resolution Imaging Offers Improved Accuracy in Neuron Tracing 

      Lakadamyali, Melike; Babcock, Hazen P.; Bates, Mark; Zhuang, Xiaowei; Lichtman, Jeff (Public Library of Science, 2012)
      The connectivity among neurons holds the key to understanding brain function. Mapping neural connectivity in brain circuits requires imaging techniques with high spatial resolution to facilitate neuron tracing and high ...
    • Chromatin topology is coupled to Polycomb group protein subnuclear organization 

      Wani, Ajazul H.; Boettiger, Alistair N.; Schorderet, Patrick; Ergun, Ayla; Münger, Christine; Sadreyev, Ruslan I.; Zhuang, Xiaowei; Kingston, Robert E.; Francis, Nicole J. (Nature Publishing Group, 2016)
      The genomes of metazoa are organized at multiple scales. Many proteins that regulate genome architecture, including Polycomb group (PcG) proteins, form subnuclear structures. Deciphering mechanistic links between protein ...
    • Clathrin and AP2 are required for PtdIns(4,5)P2-mediated formation of LRP6 signalosomes 

      Kim, Ingyu; Pan, Weijun; Jones, Sara A.; Zhang, Youxin; Zhuang, Xiaowei; Wu, Dianqing (The Rockefeller University Press, 2013)
      Canonical Wnt signaling is initiated by the binding of Wnt proteins to their receptors, low-density lipoprotein-related protein 5 and 6 (LRP5/6) and frizzled proteins, leading to phosphatidylinositol (4,5)bisphosphate ...
    • Correlative Stochastic Optical Reconstruction Microscopy and Electron Microscopy 

      Kim, Doory; Deerinck, Thomas J.; Sigal, Yaron M.; Babcock, Hazen P.; Ellisman, Mark H.; Zhuang, Xiaowei (Public Library of Science, 2015)
      Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure. Recent development of super-resolution ...
    • Development of the CRISPR nuclease Cas9 for high precision mammalian genome engineering 

      Hsu, Patrick David (2014-10-21)
      Recent advances in genome engineering technologies based on the CRISPR-associated RNA-guided endonuclease Cas9 are enabling the systematic interrogation of genome function. Analogous to the search function in modern word ...
    • Developmental mechanism of the periodic membrane skeleton in axons 

      Zhong, Guisheng; He, Jiang; Zhou, Ruobo; Lorenzo, Damaris; Babcock, Hazen P; Bennett, Vann; Zhuang, Xiaowei (eLife Sciences Publications, Ltd, 2014)
      Actin, spectrin, and associated molecules form a periodic sub-membrane lattice structure in axons. How this membrane skeleton is developed and why it preferentially forms in axons are unknown. Here, we studied the developmental ...
    • Dissecting the Cell Entry Pathway of Dengue Virus by Single-Particle Tracking in Living Cells 

      van der Schaar, Hilde M.; van der Ende-Metselaar, Heidi; Wilschut, Jan; Smit, Jolanda M.; Rust, Michael Joseph; Chen, Chen; Zhuang, Xiaowei (Public Library of Science, 2008)
      Dengue virus (DENV) is an enveloped RNA virus that causes the most common arthropod-borne infection worldwide. The mechanism by which DENV infects the host cell remains unclear. In this work, we used live-cell imaging and ...
    • Dual Function of CD81 in Influenza Virus Uncoating and Budding 

      He, Jiang; Sun, Eileen; Bujny, Miriam V.; Kim, Doory; Davidson, Michael W.; Zhuang, Xiaowei (Public Library of Science, 2013)
      As an obligatory pathogen, influenza virus co-opts host cell machinery to harbor infection and to produce progeny viruses. In order to characterize the virus-host cell interactions, several genome-wide siRNA screens and ...
    • High-performance probes for light and electron microscopy 

      Viswanathan, Sarada; Williams, Megan E.; Bloss, Erik B.; Stasevich, Timothy J.; Speer, Colenso M.; Nern, Aljoscha; Pfeiffer, Barret D.; Hooks, Bryan M.; Li, Wei-Ping; English, Brian P.; Tian, Teresa; Henry, Gilbert L.; Macklin, John J.; Patel, Ronak; Gerfen, Charles R.; Zhuang, Xiaowei; Wang, Yalin; Rubin, Gerald M.; Looger, Loren L. (2015)
      We describe an engineered family of highly antigenic molecules based on GFP-like fluorescent proteins. These molecules contain numerous copies of peptide epitopes and simultaneously bind IgG antibodies at each location. ...
    • Histone H4 tail mediates allosteric regulation of nucleosome remodelling by linker DNA 

      Hwang, William L.; Deindl, Sebastian; Harada, Bryan T.; Zhuang, Xiaowei (2014)
      ISWI-family remodelling enzymes regulate access to genomic DNA by mobilizing nucleosomes1. These ATP-dependent chromatin remodellers promote heterochromatin formation and transcriptional silencing1 by generating regularly-spaced ...
    • Imaging Poliovirus Entry in Live Cells 

      Brandenburg, Boerries; Lee, Lily Y.; Lakadamyali, Melike; Rust, Michael J.; Zhuang, Xiaowei; Hogle, James M. (Public Library of Science, 2007)
      Viruses initiate infection by transferring their genetic material across a cellular membrane and into the appropriate compartment of the cell. The mechanisms by which animal viruses, especially nonenveloped viruses, deliver ...
    • Isotropic 3D Super-resolution Imaging with a Self-bending Point Spread Function 

      Jia, Shu; Vaughan, Joshua C.; Zhuang, Xiaowei (2014)
      Airy beams maintain their intensity profiles over a large propagation distance without substantial diffraction and exhibit lateral bending during propagation1-5. This unique property has been exploited for micromanipulation ...
    • The Mechanism and Regulation of Chromatin Remodeling by ISWI Family Enzymes 

      Hwang, William Liang (2013-09-24)
      Eukaryotic genomes are packaged as chromatin, which restricts access to the DNA by critical processes such as DNA replication, repair, and transcription. As a result, eukaryotic cells rely on ATP-dependent chromatin ...
    • Monovalent engagement of the BCR activates ovalbumin-specific transnuclear B cells 

      Avalos, Ana M.; Bilate, Angelina M.; Witte, Martin D.; Tai, Albert K.; He, Jiang; Frushicheva, Maria P.; Thill, Peter D.; Meyer-Wentrup, Friederike; Theile, Christopher S.; Chakraborty, Arup K.; Zhuang, Xiaowei; Ploegh, Hidde L. (The Rockefeller University Press, 2014)
      Valency requirements for B cell activation upon antigen encounter are poorly understood. OB1 transnuclear B cells express an IgG1 B cell receptor (BCR) specific for ovalbumin (OVA), the epitope of which can be mimicked ...
    • Morphological, Physiological and Molecular Classification of Mouse Retinal Ganglion Cells 

      Qiao, Mu (2016-01-15)
      Visual information is conveyed from the retina to the brain through axons of retinal ganglion cells (RGCs). There are >20 different subtypes of RGCs, each of which detects specific features. Classification of RGC subtypes ...
    • Nanoscale Organization and Optical Observation of Biomolecules With DNA Nanotechnology 

      Dai, Mingjie (2016-05-19)
      Understanding biomolecular information at the single-molecule level requires tools for manipulating and observing individual biomolecules at the nanoscale. Programmable DNA nanotechnology provides an ideal interface to ...
    • Nanotools for Neuroscience and Brain Activity Mapping 

      Alivisatos, A. Paul; Andrews, Anne M.; Boyden, Edward S.; Chun, Miyoung; Church, George McDonald; Deisseroth, Karl; Donoghue, John P.; Fraser, Scott E.; Lippincott-Schwartz, Jennifer; Looger, Loren L.; Masmanidis, Sotiris; McEuen, Paul L.; Nurmikko, Arto V.; Park, Hongkun; Peterka, Darcy S.; Reid, Clay; Roukes, Michael L.; Scherer, Axel; Schnitzer, Mark; Sejnowski, Terrence J.; Shepard, Kenneth L.; Tsao, Doris; Turrigiano, Gina; Weiss, Paul; Xu, Chris; Yuste, Rafael; Zhuang, Xiaowei (American Chemical Society (ACS), 2013)
      Neuroscience is at a crossroads. Great effort is being invested into deciphering specific neural interactions and circuits. At the same time, there exist few general theories or principles that explain brain function. We ...
    • Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains 

      Hota, Swetansu K.; Bhardwaj, Saurabh K.; Deindl, Sebastian; Lin, Yuan-chi; Zhuang, Xiaowei; Bartholomew, Blaine (2013)
      The ISWI family of ATP-dependent chromatin remodelers represses transcription by changing nucleosome positioning. The interactions with extranucleosomal DNA and the requirement of a minimal length of extranucleosomal DNA ...
    • Photoswitchable Fluorescent Probes for Localization-Based Super-Resolution Imaging 

      Dempsey, Graham Thomas (2013-02-21)
      In recent years, localization-based super-resolution imaging has been developed to overcome the diffraction limit of far-field fluorescence microscopy. Photoswitchable probes are a hallmark of this technique. Their ...
    • A PIK3C3–Ankyrin-B–Dynactin pathway promotes axonal growth and multiorganelle transport 

      Lorenzo, Damaris Nadia; Badea, Alexandra; Davis, Jonathan; Hostettler, Janell; He, Jiang; Zhong, Guisheng; Zhuang, Xiaowei; Bennett, Vann (The Rockefeller University Press, 2014)
      Axon growth requires long-range transport of organelles, but how these cargoes recruit their motors and how their traffic is regulated are not fully resolved. In this paper, we identify a new pathway based on the class III ...