Browsing by Author "Shamu, Caroline"
Now showing items 1-3 of 3
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An Intermittent Live Cell Imaging Screen for siRNA Enhancers and Suppressors of a Kinesin-5 Inhibitor
Tsui, Melody; Xie, Tiao; Orth, James Daniel; Carpenter, Anne E.; Rudnicki, Stewart; Kim, Suejong; Shamu, Caroline Elizabeth; Mitchison, Timothy J. (Public Library of Science, 2009)Kinesin-5 (also known as Eg5, KSP and Kif11) is required for assembly of a bipolar mitotic spindle. Small molecule inhibitors of Kinesin-5, developed as potential anti-cancer drugs, arrest cell in mitosis and promote ... -
Online GESS: prediction of miRNA-like off-target effects in large-scale RNAi screen data by seed region analysis
Yilmazel, Bahar; Hu, Yanhui; Sigoillot, Frederic; Smith, Jennifer A; Shamu, Caroline E; Perrimon, Norbert; Mohr, Stephanie E (BioMed Central, 2014)Background: RNA interference (RNAi) is an effective and important tool used to study gene function. For large-scale screens, RNAi is used to systematically down-regulate genes of interest and analyze their roles in a ... -
RNAi Screening Comes of Age: Improved Techniques and Complementary Approaches
Mohr, Stephanie; Smith, Jennifer; Shamu, Caroline; Neumüller, Ralph A.; Perrimon, Norbert (Springer Science and Business Media LLC, 2014-09)Gene silencing through sequence-specific targeting of mRNAs by RNAi has enabled genome-wide functional screens in cultured cells and in vivo in model organisms. These screens have resulted in the identification of new ...