Now showing items 1-15 of 15

    • An affinity-based scoring scheme for predicting DNA-binding activities of modularly assembled zinc-finger proteins 

      Sander, Jeffry D; Zaback, Peter; Joung, Jae Keith; Voytas, Daniel F.; Dobbs, Drena (Oxford University Press, 2009)
      Zinc-finger proteins (ZFPs) have long been recognized for their potential to manipulate genetic information because they can be engineered to bind novel DNA targets. Individual zinc-finger domains (ZFDs) bind specific DNA ...
    • Autonomous Zinc-Finger Nuclease Pairs for Targeted Chromosomal Deletion 

      Şöllü, Cem; Pars, Kaweh; Cornu, Tatjana I.; Thibodeau-Beganny, Stacey; Maeder, Morgan L.; Joung, Jae Keith; Heilbronn, Regine; Cathomen, Toni (Oxford University Press, 2010)
      Zinc-finger nucleases (ZFNs) have been successfully used for rational genome engineering in a variety of cell types and organisms. ZFNs consist of a non-specific FokI endonuclease domain and a specific zinc-finger DNA-binding ...
    • Engineered DNA-Binding Proteins for Targeted Genome Editing and Gene Regulation 

      Maeder, Morgan Lee (2013-10-08)
      Engineered DNA-binding proteins enable targeted manipulation of the genome. Zinc fingers are the most well characterized DNA-binding domain and for many years research has focused on understanding and manipulating the ...
    • Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects 

      Ramirez, Cherie Lynn; Certo, Michael T.; Mussolino, Claudio; Goodwin, Matthew J.; Cradick, Thomas J.; McCaffrey, Anton P.; Cathomen, Toni; Scharenberg, Andrew M.; Joung, Jae Keith (Oxford University Press, 2012)
      Engineered zinc finger nucleases (ZFNs) induce DNA double-strand breaks at specific recognition sequences and can promote efficient introduction of desired insertions, deletions or substitutions at or near the cut site via ...
    • Evaluation of OPEN Zinc Finger Nucleases for Direct Gene Targeting of the ROSA26 Locus in Mouse Embryos 

      Hermann, Mario; Rector, Kyle; Ruiz, Joseph; Becher, Burkhard; Bürki, Kurt; Khayter, Cyd; Aguzzi, Adriano; Buch, Thorsten; Pelczar, Pawel; Maeder, Morgan L.; Joung, Jae Keith (Public Library of Science, 2012)
      Zinc finger nucleases (ZFNs) enable precise genome modification in a variety of organisms and cell types. Commercial ZFNs were reported to enhance gene targeting directly in mouse zygotes, whereas similar approaches using ...
    • Highly Efficient Generation of Heritable Zebrafish Gene Mutations Using Homo- and Heterodimeric TALENs 

      Cade, Lindsay; Reyon, Deepak; Hwang, Woong Y.; Tsai, Shengdar; Patel, Samir; Khayter, Cyd; Joung, Jae Keith; Sander, Jeffry D; Peterson, Randall Theodore; Yeh, Jing-Ruey Joanna (Oxford University Press, 2012)
      Transcription activator-like effector nucleases (TALENs) are powerful new research tools that enable targeted gene disruption in a wide variety of model organisms. Recent work has shown that TALENs can induce mutations in ...
    • Identification of promoter targets of enhancers by epigenetic knockdown using TAL DNA binding proteins 

      Mendenhall, Eric M; Williamson, Kaylyn Elise; Reyon, Deepak; Joung, Jae Keith; Bernstein, Bradley E. (BioMed Central, 2013)
    • Improved Somatic Mutagenesis in Zebrafish Using Transcription Activator-Like Effector Nucleases (TALENs) 

      Moore, Finola Elizabeth; Reyon, Deepak; Sander, Jeffry D.; Martinez, Sarah A.; Blackburn, Jessica S.; Khayter, Cyd; Ramirez, Cherie Lynn; Joung, Jae Keith; Langenau, David M. (Public Library of Science, 2012)
      Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-Like Effector Nucleases (TALENs) provide robust and user-friendly technologies for efficiently inactivating genes in ...
    • Improving Zinc Finger Nucleases - Strategies for Increasing Gene Editing Activities and Evaluating Off-Target Effects 

      Ramirez, Cherie Lynn (2012-08-23)
      Zinc finger nucleases (ZFNs) induce double-strand DNA breaks at specific recognition sites. ZFNs can dramatically increase the efficiency of incorporating desired insertions, deletions, or substitutions in living cells. ...
    • Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1 

      Kiskinis, Evangelos; Sandoe, Jackson L; Williams, Lauren N.; Boulting, Gabriella Lutz; Moccia, Robert; Wainger, Brian Jason; Han, Steve Sang-woo; Peng, Theodore; Thams, Sebastian; Mikkilineni, Shravani; Mellin, Cassidy; Merkle, Florian Tobias; Davis-Dusenbery, B; Ziller, Michael Johannes; Oakley, Derek Hayden; Ichida, Justin; Di Costanzo, Stefania; Atwater, Nick; Maeder, M; Goodwin, Marcus; Nemesh, James; Handsaker, Robert E; Paull, Daniel; Noggle, Scott; McCarroll, Steven A.; Joung, Jae Keith; Woolf, Carl; Brown, Robert H; Eggan, Kevin Carl (Elsevier BV, 2014)
      Direct electrical recording and stimulation of neural activity using micro-fabricated silicon and metal micro-wire probes have contributed extensively to basic neuroscience and therapeutic applications; however, the ...
    • Profiling the DNA-Binding Specificities of Engineered Cys2His2 Zinc Finger Domains Using a Rapid Cell-Based Method 

      Meng, Xiangdong; Thibodeau-Beganny, Stacey; Jiang, Tao; Joung, Jae Keith; Wolfe, Scot A. (Oxford University Press, 2007)
      The C2H2 zinc finger is the most commonly utilized framework for engineering DNA-binding domains with novel specificities. Many different selection strategies have been developed to identify individual fingers that possess ...
    • Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN) 

      Foley, Jonathan E.; Yeh, Jing-Ruey Joanna; Maeder, Morgan L.; Reyon, Deepak; Sander, Jeffry D; Peterson, Randall Theodore; Joung, Jae Keith (Public Library of Science, 2009)
      Background: Customized zinc finger nucleases (ZFNs) form the basis of a broadly applicable tool for highly efficient genome modification. ZFNs are artificial restriction endonucleases consisting of a non-specific nuclease ...
    • Translating the Genomics Revolution: The Need for an International Gene Therapy Consortium for Monogenic Diseases 

      Tremblay, Jacques P; Xiao, Xiao; Aartsma-Rus, Annemieke; Barbas, Carlos; Blau, Helen M; Bogdanove, Adam J; Boycott, Kym; Braun, Serge; Breakefield, Xandra Owens; Bueren, Juan A; Buschmann, Michael; Byrne, Barry J; Calos, Michele; Cathomen, Toni; Chamberlain, Jeffrey; Chuah, Marinee; Cornetta, Kenneth; Davies, Kay E; Dickson, J George; Duchateau, Philippe; Flotte, Terence R; Gaudet, Daniel; Gersbach, Charles A; Gilbert, Renald; Glorioso, Joseph; Herzog, Roland W; High, Katherine A; Huang, Wenlin; Huard, Johnny; Joung, Jae Keith; Liu, Depei; Liu, Dexi; Lochmüller, Hanns; Lustig, Lawrence; Martens, Jeffrey; Massie, Bernard; Mavilio, Fulvio; Mendell, Jerry R; Nathwani, Amit; Ponder, Katherine; Porteus, Matthew; Puymirat, Jack; Samulski, Jude; Takeda, Shin'ichi; Thrasher, Adrian; VandenDriessche, Thierry; Wei, Yuquan; Wilson, James M; Wilton, Steve D; Wolfe, John H; Gao, Guangping (Nature Publishing Group, 2013)
    • Zinc Finger Database (ZiFDB): A Repository for Information on C2H2 Zinc Fingers and Engineered Zinc-Finger Arrays 

      Fu, Fengli; Sander, Jeffry D; Maeder, Morgan; Thibodeau-Beganny, Stacey; Joung, Jae Keith; Dobbs, Drena; Miller, Leslie; Voytas, Daniel F. (Oxford University Press, 2009)
      Zinc fingers are the most abundant DNA-binding motifs encoded by eukaryotic genomes and one of the best understood DNA-recognition domains. Each zinc finger typically binds a 3-nt target sequence, and it is possible to ...
    • Zinc Finger Targeter (ZiFiT): An Engineered Zinc Finger/Target Site Design Tool 

      Sander, Jeffry D; Zaback, Peter; Joung, Jae Keith; Voytas, Daniel F.; Dobbs, Drena (Oxford University Press, 2007)
      Zinc Finger Targeter (ZiFiT) is a simple and intuitive web-based tool that facilitates the design of zinc finger proteins (ZFPs) that can bind to specific DNA sequences. The current version of ZiFiT is based on a widely ...