Now showing items 1-18 of 18

    • An affinity-based scoring scheme for predicting DNA-binding activities of modularly assembled zinc-finger proteins 

      Sander, Jeffry D; Zaback, Peter; Joung, Jae Keith; Voytas, Daniel F.; Dobbs, Drena (Oxford University Press, 2009)
      Zinc-finger proteins (ZFPs) have long been recognized for their potential to manipulate genetic information because they can be engineered to bind novel DNA targets. Individual zinc-finger domains (ZFDs) bind specific DNA ...
    • Augmenting and Directing Robust, Long-Range CRISPR-Mediated Activation in Human Cells 

      Tak, Y. Esther; Horng, Joy E.; Perry, Nicholas T.; Schultz, Hayley; Iyer, Sowmya; Yao, Qiuming; Zou, Luli S.; Aryee, Martin; Pinello, Luca; Joung, Keith (Springer Science and Business Media LLC, 2021-08-05)
      Epigenetic editing is an emerging technology that uses artificial transcription factors (aTFs) to regulate expression of a target gene. Although human genes can be robustly upregulated by targeting aTFs to promoters, the ...
    • Autonomous Zinc-Finger Nuclease Pairs for Targeted Chromosomal Deletion 

      Şöllü, Cem; Pars, Kaweh; Cornu, Tatjana I.; Thibodeau-Beganny, Stacey; Maeder, Morgan L.; Joung, Jae Keith; Heilbronn, Regine; Cathomen, Toni (Oxford University Press, 2010)
      Zinc-finger nucleases (ZFNs) have been successfully used for rational genome engineering in a variety of cell types and organisms. ZFNs consist of a non-specific FokI endonuclease domain and a specific zinc-finger DNA-binding ...
    • Engineered DNA-Binding Proteins for Targeted Genome Editing and Gene Regulation 

      Maeder, Morgan Lee (2013-10-08)
      Engineered DNA-binding proteins enable targeted manipulation of the genome. Zinc fingers are the most well characterized DNA-binding domain and for many years research has focused on understanding and manipulating the ...
    • Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects 

      Ramirez, Cherie Lynn; Certo, Michael T.; Mussolino, Claudio; Goodwin, Matthew J.; Cradick, Thomas J.; McCaffrey, Anton P.; Cathomen, Toni; Scharenberg, Andrew M.; Joung, Jae Keith (Oxford University Press, 2012)
      Engineered zinc finger nucleases (ZFNs) induce DNA double-strand breaks at specific recognition sequences and can promote efficient introduction of desired insertions, deletions or substitutions at or near the cut site via ...
    • Evaluation of OPEN Zinc Finger Nucleases for Direct Gene Targeting of the ROSA26 Locus in Mouse Embryos 

      Hermann, Mario; Rector, Kyle; Ruiz, Joseph; Becher, Burkhard; Bürki, Kurt; Khayter, Cyd; Aguzzi, Adriano; Buch, Thorsten; Pelczar, Pawel; Maeder, Morgan L.; Joung, Jae Keith (Public Library of Science, 2012)
      Zinc finger nucleases (ZFNs) enable precise genome modification in a variety of organisms and cell types. Commercial ZFNs were reported to enhance gene targeting directly in mouse zygotes, whereas similar approaches using ...
    • Highly Efficient Generation of Heritable Zebrafish Gene Mutations Using Homo- and Heterodimeric TALENs 

      Cade, Lindsay; Reyon, Deepak; Hwang, Woong Y.; Tsai, Shengdar; Patel, Samir; Khayter, Cyd; Joung, Jae Keith; Sander, Jeffry D; Peterson, Randall Theodore; Yeh, Jing-Ruey Joanna (Oxford University Press, 2012)
      Transcription activator-like effector nucleases (TALENs) are powerful new research tools that enable targeted gene disruption in a wide variety of model organisms. Recent work has shown that TALENs can induce mutations in ...
    • Identification of promoter targets of enhancers by epigenetic knockdown using TAL DNA binding proteins 

      Mendenhall, Eric M; Williamson, Kaylyn Elise; Reyon, Deepak; Joung, Jae Keith; Bernstein, Bradley E. (BioMed Central, 2013)
    • Improved Somatic Mutagenesis in Zebrafish Using Transcription Activator-Like Effector Nucleases (TALENs) 

      Moore, Finola Elizabeth; Reyon, Deepak; Sander, Jeffry D.; Martinez, Sarah A.; Blackburn, Jessica S.; Khayter, Cyd; Ramirez, Cherie Lynn; Joung, Jae Keith; Langenau, David M. (Public Library of Science, 2012)
      Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-Like Effector Nucleases (TALENs) provide robust and user-friendly technologies for efficiently inactivating genes in ...
    • Improving Zinc Finger Nucleases - Strategies for Increasing Gene Editing Activities and Evaluating Off-Target Effects 

      Ramirez, Cherie Lynn (2012-08-23)
      Zinc finger nucleases (ZFNs) induce double-strand DNA breaks at specific recognition sites. ZFNs can dramatically increase the efficiency of incorporating desired insertions, deletions, or substitutions in living cells. ...
    • Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1 

      Kiskinis, Evangelos; Sandoe, Jackson L; Williams, Lauren N.; Boulting, Gabriella Lutz; Moccia, Robert; Wainger, Brian Jason; Han, Steve Sang-woo; Peng, Theodore; Thams, Sebastian; Mikkilineni, Shravani; Mellin, Cassidy; Merkle, Florian Tobias; Davis-Dusenbery, B; Ziller, Michael Johannes; Oakley, Derek Hayden; Ichida, Justin; Di Costanzo, Stefania; Atwater, Nick; Maeder, M; Goodwin, Marcus; Nemesh, James; Handsaker, Robert E; Paull, Daniel; Noggle, Scott; McCarroll, Steven A.; Joung, Jae Keith; Woolf, Carl; Brown, Robert H; Eggan, Kevin Carl (Elsevier BV, 2014)
      Direct electrical recording and stimulation of neural activity using micro-fabricated silicon and metal micro-wire probes have contributed extensively to basic neuroscience and therapeutic applications; however, the ...
    • Profiling the DNA-Binding Specificities of Engineered Cys2His2 Zinc Finger Domains Using a Rapid Cell-Based Method 

      Meng, Xiangdong; Thibodeau-Beganny, Stacey; Jiang, Tao; Joung, Jae Keith; Wolfe, Scot A. (Oxford University Press, 2007)
      The C2H2 zinc finger is the most commonly utilized framework for engineering DNA-binding domains with novel specificities. Many different selection strategies have been developed to identify individual fingers that possess ...
    • Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN) 

      Foley, Jonathan E.; Yeh, Jing-Ruey Joanna; Maeder, Morgan L.; Reyon, Deepak; Sander, Jeffry D; Peterson, Randall Theodore; Joung, Jae Keith (Public Library of Science, 2009)
      Background: Customized zinc finger nucleases (ZFNs) form the basis of a broadly applicable tool for highly efficient genome modification. ZFNs are artificial restriction endonucleases consisting of a non-specific nuclease ...
    • Therapeutic Base Editing of Human Hematopoietic Stem Cells 

      Zeng, Jing; Wu, Yuxuan; Ren, Chunyan; Bonanno, Jasmine; Shen, Anne H.; Shea, Devlin; Gehrke, Jason M.; Clement, Kendell; Luk, Kevin; Yao, Qiuming; Kim, Rachel; Wolfe, Scot A.; Manis, John; Pinello, Luca; Joung, Keith; Bauer, Daniel (Springer Science and Business Media LLC, 2020-03-16)
      Base editing by nucleotide deaminases linked to programmable DNA-binding proteins represents a promising approach to permanently remedy blood disorders, although its application in engrafting hematopoietic stem cells (HSCs) ...
    • Transcriptome-Wide Off-Target RNA Editing Induced by CRISPR-Guided DNA Base Editors 

      Grünewald, Julian; Zhou, Ronghao; Garcia, Sara; Iyer, Sowmya; Lareau, Caleb; Aryee, Martin; Joung, Keith (Springer Science and Business Media LLC, 2019-04-17)
      CRISPR-Cas base editor technology enables targeted nucleotide alterations and is being rapidly deployed for research and potential therapeutic applications. The most widely used base editors induce DNA cytosine (C) deamination ...
    • Translating the Genomics Revolution: The Need for an International Gene Therapy Consortium for Monogenic Diseases 

      Tremblay, Jacques P; Xiao, Xiao; Aartsma-Rus, Annemieke; Barbas, Carlos; Blau, Helen M; Bogdanove, Adam J; Boycott, Kym; Braun, Serge; Breakefield, Xandra Owens; Bueren, Juan A; Buschmann, Michael; Byrne, Barry J; Calos, Michele; Cathomen, Toni; Chamberlain, Jeffrey; Chuah, Marinee; Cornetta, Kenneth; Davies, Kay E; Dickson, J George; Duchateau, Philippe; Flotte, Terence R; Gaudet, Daniel; Gersbach, Charles A; Gilbert, Renald; Glorioso, Joseph; Herzog, Roland W; High, Katherine A; Huang, Wenlin; Huard, Johnny; Joung, Jae Keith; Liu, Depei; Liu, Dexi; Lochmüller, Hanns; Lustig, Lawrence; Martens, Jeffrey; Massie, Bernard; Mavilio, Fulvio; Mendell, Jerry R; Nathwani, Amit; Ponder, Katherine; Porteus, Matthew; Puymirat, Jack; Samulski, Jude; Takeda, Shin'ichi; Thrasher, Adrian; VandenDriessche, Thierry; Wei, Yuquan; Wilson, James M; Wilton, Steve D; Wolfe, John H; Gao, Guangping (Nature Publishing Group, 2013)
    • Zinc Finger Database (ZiFDB): A Repository for Information on C2H2 Zinc Fingers and Engineered Zinc-Finger Arrays 

      Fu, Fengli; Sander, Jeffry D; Maeder, Morgan; Thibodeau-Beganny, Stacey; Joung, Jae Keith; Dobbs, Drena; Miller, Leslie; Voytas, Daniel F. (Oxford University Press, 2009)
      Zinc fingers are the most abundant DNA-binding motifs encoded by eukaryotic genomes and one of the best understood DNA-recognition domains. Each zinc finger typically binds a 3-nt target sequence, and it is possible to ...
    • Zinc Finger Targeter (ZiFiT): An Engineered Zinc Finger/Target Site Design Tool 

      Sander, Jeffry D; Zaback, Peter; Joung, Jae Keith; Voytas, Daniel F.; Dobbs, Drena (Oxford University Press, 2007)
      Zinc Finger Targeter (ZiFiT) is a simple and intuitive web-based tool that facilitates the design of zinc finger proteins (ZFPs) that can bind to specific DNA sequences. The current version of ZiFiT is based on a widely ...