Now showing items 1-5 of 5

    • Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9 

      Mandal, Pankaj Kumar; Ferreira, Leonardo Manuel Ramos; Collins, Ryan; Meissner, Torsten B; Boutwell, C; Friesen, Max; Vrbanac, Vladimir; Garrison, Brian Scott; Stortchevoi, Alexei; Bryder, David; Musunuru, Kiran; Brand, Harrison Andrew; Tager, Andrew Martin; Allen, Todd; Talkowski, Michael Edward; Rossi, Derrick J; Cowan, Chad A. (Elsevier BV, 2014)
      Genome editing via CRISPR/Cas9 has rapidly become the tool of choice by virtue of its efficacy and ease of use. However, CRISPR/Cas9-mediated genome editing in clinically relevant human somatic cells remains untested. Here, ...
    • Highly Sensitive and Specific Detection of Rare Variants in Mixed Viral Populations from Massively Parallel Sequence Data 

      Macalalad, Alexander R.; Zody, Michael; Charlebois, Patrick; Lennon, Niall J.; Newman, Ruchi M.; Malboeuf, Christine M.; Ryan, Elizabeth Marie; Boutwell, Christian Lane; Power, Karen A.; Brackney, Doug E.; Pesko, Kendra N.; Levin, Joshua Zvi; Ebel, Gregory D.; Allen, Todd; Birren, Bruce W.; Henn, Matthew R. (Public Library of Science, 2012)
      Viruses diversify over time within hosts, often undercutting the effectiveness of host defenses and therapeutic interventions. To design successful vaccines and therapeutics, it is critical to better understand viral ...
    • P09-20 LB. Ultra-Deep Sequencing of Full-Length HIV-1 Genomes Identifies Rapid Viral Evolution During Acute Infection 

      Henn, MR; Lennon, N; Malboeuf, C; Charlebois, P; Philips, L; Berical, A; Erlich, R; Kemper, M; Axten, K; Brumme, Z; Brumme, C; Jessen, H; Nusbaum, C; Birren, B; Allen, TM; Boutwell, Christian Lane; Power, K; Gladden, Andrew B; Levin, Jonathan M.; Casali, Monica; Berlin, A; Anderson, S; Streeck, Hendrik; Ryan, Edward Thomas; Wang, Y; Green, L; Russ, Christiana Marie; Rosenberg, Eric Scott; Altfeld, Marcus; Walker, Bruce David (BioMed Central, 2009)
    • Reduced Viral Replication Capacity of Human Immunodeficiency Virus Type 1 Subtype C Caused by Cytotoxic-T-Lymphocyte Escape Mutations in HLA-B57 Epitopes of Capsid Protein 

      Boutwell, C; Rowley, Christopher Francis; Essex, Myron Elmer (American Society for Microbiology, 2008)
      Cytotoxic-T-lymphocyte (CTL) escape mutations in human immunodeficiency viruses encode amino acid substitutions in positions that disrupt CTL targeting, thereby increasing virus survival and conferring a relative fitness ...
    • Whole Genome Deep Sequencing of HIV-1 Reveals the Impact of Early Minor Variants Upon Immune Recognition During Acute Infection 

      Henn, Matthew R.; Charlebois, Patrick; Lennon, Niall J.; Power, Karen A.; Macalalad, Alexander R.; Berlin, Aaron M.; Malboeuf, Christine M.; Gnerre, Sante; Erlich, Rachel L.; Green, Lisa M.; Berical, Andrew; Wang, Yaoyu; Newman, Ruchi; Axten, Karen L.; Gladden, Adrianne D.; Battis, Laura; Kemper, Michael; Zeng, Qiandong; Shea, Terrance P.; Gujja, Sharvari; Zedlack, Carmen; Gasser, Olivier; Brander, Christian; Günthard, Huldrych F.; Brumme, Zabrina L.; Brumme, Chanson J.; Bazner, Suzane; Rychert, Jenna; Tinsley, Jake P.; Levin, Joshua Z.; Jessen, Heiko; Birren, Bruce W.; Boutwell, Christian Lane; Ryan, Elizabeth M.; Zody, Michael C.; Casali, Monica; Streeck, Hendrik; Bloom, Allyson Kelly; Dudek, Timothy E.; Tully, Damien C.; Hess, Christoph; Mayer, Kenneth Hugh; Rosenberg, Eric Scott; Pereyra, Florencia M.; Young, Sarah K.; Altfeld, Marcus; Walker, Bruce David; Allen, Todd (Public Library of Science, 2012)
      Deep sequencing technologies have the potential to transform the study of highly variable viral pathogens by providing a rapid and cost-effective approach to sensitively characterize rapidly evolving viral quasispecies. ...