Browsing by Author "Ewen-Campen, Benjamin"

Now showing items 1-9 of 9

    • BMP signaling is required for the generation of primordial germ cells in an insect 

      Donoughe, Seth T; Nakamura, Taro; Ewen-Campen, Benjamin Scott; Green, D; Henderson, Lory O.; Extavour, Cassandra G. (Proceedings of the National Academy of Sciences, 2014)
      Many model organisms specify germ cells using maternally supplied germ-line determinants. In contrast, mice rely on embryonic cell–cell signaling to induce cells to become germ cells. Molecular evidence for inductive ...

    • CRISPR Guide RNA Design for Research Applications 

      Mohr, Stephanie; Hu, Yanhui; Ewen-Campen, Benjamin; Housden, Benjamin; Viswanatha, Raghuvir; Perrimon, Norbert (Wiley-Blackwell, 2016-09)
      The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools that facilitate design of on‐target and effective guide RNAs ...

    • Developmental Gene Discovery in a Hemimetabolous Insect: De Novo Assembly and Annotation of a Transcriptome for the Cricket Gryllus Bimaculatus 

      Zeng, Victor; Ewen-Campen, Benjamin Scott; Horch, Hadley W.; Roth, Siegfried; Mito, Taro; Extavour, Cassandra G. (Public Library of Science, 2013-04-05)
      Most genomic resources available for insects represent the Holometabola, which are insects that undergo complete metamorphosis like beetles and flies. In contrast, the Hemimetabola (direct developing insects), representing ...

    • Evidence against a Germ Plasm in the Milkweed Bug Oncopeltus fasciatus, a Hemimetabolous Insect 

      Ewen-Campen, Benjamin Scott; Jones, Tasmin E. M.; Extavour, Cassandra G. (Company of Biologists, 2013-03-07)
      Primordial germ cell (PGC) formation in holometabolous insects like Drosophila melanogaster relies on maternally synthesised germ cell determinants that are asymmetrically localised to the oocyte posterior cortex. Embryonic ...

    • An evolutionary perspective on germ cell specification genes in insects 

      Ewen-Campen, Benjamin Scott (2014-06-06)
      This dissertation investigates the embryonic specification of a specific group of cells: the germ cells. Germ cells, which give rise to sperm and egg, are the only cells in sexually-reproducing animals that directly ...

    • The Maternal and Early Embryonic Transcriptome of the Milkweed Bug Oncopeltus fasciatus 

      Ewen-Campen, Benjamin Scott; Shaner, Nathan; Panfilio, Kristen A.; Suzuki, Yuichiro; Roth, Siegfried; Extavour, Cassandra G. (BioMed Central, 2011)
      Background. Most evolutionary developmental ("evo-devo") studies of emerging model organisms focus on small numbers of candidate genes cloned individually using degenerate PCR. However, newly available sequencing technologies ...

    • The Molecular Machinery of Germ Line Specification 

      Ewen-Campen, Benjamin Scott; Schwager, Evelyn E.; Extavour, Cassandra G. (Wiley-Blackwell, 2009)
      Germ cells occupy a unique position in animal reproduction, development, and evolution. In sexually reproducing animals, only they can produce gametes and contribute genetically to subsequent generations. Nonetheless, germ ...

    • De Novo Assembly and Characterization of a Maternal and Developmental Transcriptome for the Emerging Model Crustacean Parhyale hawaiensis 

      Zeng, Victor; Villanueva, Karina E; Ewen-Campen, Benjamin Scott; Alwes, Frederike; Browne, William E; Extavour, Cassandra G. (BioMed Central, 2011)
      Background: Arthropods are the most diverse animal phylum, but their genomic resources are relatively few. While the genome of the branchiopod Daphnia pulex is now available, no other large-scale crustacean genomic resources ...

    • Optimized Strategy for in Vivo Cas9-Activation in Drosophila 

      Ewen-Campen, Benjamin; Yang-Zhou, Donghui; Fernandes, Vitória R.; González, Delfina P.; Liu, Lu-Ping; Tao, Rong; Ren, Xingjie; Sun, Jin; Hu, Yanhui; Zirin, Jonathan; Mohr, Stephanie; Ni, Jian-Quan; Perrimon, Norbert (National Academy of Sciences, 2017-08-29)
      While several large-scale resources are available for in vivo loss-of-function studies in Drosophila, an analogous resource for overexpressing genes from their endogenous loci does not exist. We describe a strategy for ...