Now showing items 1-18 of 18

    • An authentic imaging probe to track cell fate from beginning to end 

      Lee, Seung Koo; Mortensen, Luke J.; Lin, Charles P.; Tung, Ching-Hsuan (2015)
      Accurate tracing of cell viability is critical for optimizing delivery methods, and evaluating the efficacy and safety of cell therapeutics. A nanoparticle-based cell tracker is developed to image cell fate from live to ...
    • Blood Accessibility to Fibrin in Venous Thrombosis is Thrombus Age-Dependent and Predicts Fibrinolytic Efficacy: An In Vivo Fibrin Molecular Imaging Study 

      Stein-Merlob, Ashley F.; Kessinger, Chase W.; Erdem, S. Sibel; Zelada, Henry; Hilderbrand, Scott A.; Lin, Charles P.; Tearney, Guillermo J.; Jaff, Michael R.; Reed, Guy L.; Henke, Peter K.; McCarthy, Jason R.; Jaffer, Farouc A. (Ivyspring International Publisher, 2015)
      Fibrinolytic therapy of venous thromboembolism (VTE) is increasingly utilized, yet limited knowledge is available regarding in vivo mechanisms that govern fibrinolytic efficacy. In particular, it is unknown how age-dependent ...
    • Chronic variable stress activates hematopoietic stem cells 

      Heidt, Timo; Sager, Hendrik B.; Courties, Gabriel; Dutta, Partha; Iwamoto, Yoshiko; Zaltsman, Alex; von zur Muhlen, Constantin; Bode, Christoph; Fricchione, Gregory L.; Denninger, John; Lin, Charles P.; Vinegoni, Claudio; Libby, Peter; Swirski, Filip K.; Weissleder, Ralph; Nahrendorf, Matthias (2014)
      Exposure to psychosocial stress is a risk factor for many diseases, including atherosclerosis1,2. While incompletely understood, interaction between the psyche and the immune system provides one potential mechanism linking ...
    • Defining Clonal Color in Fluorescent Multi-Clonal Tracking 

      Wu, Juwell W.; Turcotte, Raphaël; Alt, Clemens; Runnels, Judith M.; Tsao, Hensin; Lin, Charles P. (Nature Publishing Group, 2016)
      Clonal heterogeneity and selection underpin many biological processes including development and tumor progression. Combinatorial fluorescent protein expression in germline cells has proven its utility for tracking the ...
    • DHODH modulates transcriptional elongation in the neural crest and melanoma 

      White, Richard Mark; Cech, Jennifer; Ratanasirintrawoot, Sutheera; Lin, Charles P.; Rahl, Peter B.; Burke, Christopher J.; Langdon, Erin; Tomlinson, Matthew L.; Mosher, Jack; Kaufman, Charles Kore; Chen, Frank; Long, Hannah K.; Kramer, Martin; Datta, Sumon Michael; Neuberg, Donna S.; Granter, Scott R.; Young, Richard A.; Morrison, Sean; Wheeler, Grant N.; Zon, Leonard Ira (Nature Publishing Group, 2011)
    • Direct measurement of local oxygen concentration in the bone marrow of live animals 

      Spencer, Joel A.; Ferraro, Francesca; Roussakis, Emmanuel; Klein, Alyssa; Wu, Juwell; Runnels, Judith M.; Zaher, Walid; Mortensen, Luke J.; Alt, Clemens; Turcotte, Raphaël; Yusuf, Rushdia; Côté, Daniel; Vinogradov, Sergei A.; Scadden, David T.; Lin, Charles P. (2014)
      Characterizing how the microenvironment, or niche, regulates stem cell activity is central to understanding stem cell biology and to developing strategies for therapeutic manipulation of stem cells1. Low oxygen tension ...
    • Distinct bone marrow blood vessels differentially regulate haematopoiesis 

      Itkin, Tomer; Gur-Cohen, Shiri; Spencer, Joel A.; Schajnovitz, Amir; Ramasamy, Saravana K.; Kusumbe, Anjali P.; Ledergor, Guy; Jung, Yookyung; Milo, Idan; Poulos, Michael G.; Kalinkovich, Alexander; Ludin, Aya; Kollet, Orit; Shakhar, Guy; Butler, Jason M.; Rafii, Shahin; Adams, Ralf H.; Scadden, David Thomas; Lin, Charles P.; Lapidot, Tsvee (Springer Nature, 2016)
      Bone marrow (BM) endothelial cells (BMECs) form a network of blood vessels (BVs) which regulate both leukocyte trafficking and hematopoietic stem and progenitor cell (HSPC) maintenance. However, it is not clear how BMECs ...
    • IKK\(\beta\) Regulates Essential Functions of the Vascular Endothelium Through Kinase-Dependent and -Independent Pathways 

      Ashida, Noboru; SenBanerjee, Sucharita B.; Kodama, Shohta; Foo, Shi Yin; Coggins, Matthew Peter; Spencer, Joel A.; Zamiri, Parisa; Shen, Dongxiao; Li, Ling; Sciuto, Tracey; Dvorak, Ann Marie; Gerszten, Robert Edgardo; Lin, Charles P.; Karin, Michael; Rosenzweig, Anthony (Nature Publishing Group, 2011)
      Vascular endothelium provides a selective barrier between the blood and tissues, participates in wound healing and angiogenesis, and regulates tissue recruitment of inflammatory cells. Nuclear factor (NF)- \(\kappa\)B ...
    • Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence 

      Li, Chunqiang; Pastila, Riikka; Pitsillides, Costas; Runnels, Judith M.; Puoris’haag, Mehron; Côté, Daniel; Lin, Charles P. (Optical Society of America, 2010)
      We describe a new method for imaging leukocytes in vivo by exciting the endogenous protein fluorescence in the ultraviolet (UV) spectral region where tryptophan is the major fluorophore. Two-photon excitation near 590 nm ...
    • Inhibiting stromal cell heparan sulfate synthesis improves stem cell mobilization and enables engraftment without cytotoxic conditioning 

      Saez, Borja; Ferraro, F.; Yusuf, Rushdia Zareen; Cook, Colleen M.; Yu, Vionnie Wing Chi; Pardo-Saganta, A.; Sykes, Stephen M.; Palchaudhuri, Rahul; Schajnovitz, Amir; Lotinun, S; Lymperi, Stefania; Mendez-Ferrer, Simon; del Toro, Raquel; Day, Robyn Rouviere; Vasic, Radovan; Acharya, Sanket Shyamkant; Baron, Roland; Lin, Charles P.; Yamaguchi, Yu; Wagers, Amy Jo; Scadden, David Thomas (American Society of Hematology, 2014)
      The glycosyltransferase gene, Ext1, is essential for heparan sulfate production. Induced deletion of Ext1 selectively in Mx1-expressing bone marrow (BM) stromal cells, a known population of skeletal stem/progenitor cells, ...
    • Myocardial Infarction Accelerates Atherosclerosis 

      Leuschner, Florian; Robbins, Clinton; Iwamoto, Yoshiko; Thompson, Brian; Carlson, Alicia L.; Heidt, Timo; Lasitschka, Felix; Etzrodt, Martin; Waterman, Peter; Waring, Michael T.; Chicoine, Adam T.; van der Laan, Anja M.; Niessen, Hans W.M.; Piek, Jan J.; Rubin, Barry B.; Butany, Jagdish; Katus, Hugo A.; Murphy, Sabina A.; Pittet, Mikael J.; Lin, Charles P.; Dutta, Partha; Courties, Gabriel P; Wei, Ying; Gorbatov, Rostic; Majmudar, Maulik D; Stone, James Robert; Morrow, David Andrew; Sabatine, Marc Steven; Vinegoni, Claudio; Moskowitz, Michael Arthur; Libby, Peter; Swirski, Filip K.; Weissleder, Ralph; Nahrendorf, Matthias (Nature Publishing Group, 2012)
      During progression of atherosclerosis, myeloid cells destabilize lipid-rich plaque in the arterial wall and cause its rupture, thus triggering myocardial infarction and stroke. Survivors of acute coronary syndromes have a ...
    • Quantification of Mesenchymal Stem Cell (MSC) Delivery to a Target Site Using In Vivo Confocal Microscopy 

      Mortensen, Luke J.; Levy, Oren; Phillips, Joseph P.; Stratton, Tara; Triana, Brian; Ruiz, Juan P.; Gu, Fangqi; Karp, Jeffrey M.; Lin, Charles P. (Public Library of Science, 2013)
      The ability to deliver cells to appropriate target tissues is a prerequisite for successful cell-based therapy. To optimize cell therapy it is therefore necessary to develop a robust method of in vivo cell delivery ...
    • Soluble Guanylate Cyclase α1–Deficient Mice: A Novel Murine Model for Primary Open Angle Glaucoma 

      Ko, Yu-Chieh; Hayton, Sarah R.; Jones, Alexander; Tainsh, Laurel T.; Ren, Ruiyi; Giani, Andrea; Clerté, Maeva; Abernathy, Emma; de Waard, Nadine; Turcotte, Raphael; Nathan, Daniel; Loomis, Stephanie J.; Gong, Haiyan; Brouckaert, Peter; Buys, Emmanuel; Alt, Clemens; Tainsh, Robert Edward Tillinghast; Oh, Dong-Jin; Malhotra, Rajeev; Arora, Pankaj; Yu, Binglan; Scherrer-Crosbie, Marielle; Kang, Jae Hee Hee; Lin, Charles P.; Rhee, Douglas J; Wiggs, Janey Lee; Gregory, Meredith S.; Pasquale, Louis; Bloch, Kenneth Daniel; Ksander, Bruce R. (Public Library of Science, 2013)
      Primary open angle glaucoma (POAG) is a leading cause of blindness worldwide. The molecular signaling involved in the pathogenesis of POAG remains unknown. Here, we report that mice lacking the \(α_1\) subunit of the nitric ...
    • Statins Improve the Resolution of Established Murine Venous Thrombosis: Reductions in Thrombus Burden and Vein Wall Scarring 

      Kessinger, Chase W.; Kim, Jin Won; Henke, Peter K.; Thompson, Brian; McCarthy, Jason R.; Hara, Tetsuya; Sillesen, Martin; Margey, Ronan J. P.; Libby, Peter; Weissleder, Ralph; Lin, Charles P.; Jaffer, Farouc A. (Public Library of Science, 2015)
      Despite anticoagulation therapy, up to one-half of patients with deep vein thrombosis (DVT) will develop the post-thrombotic syndrome (PTS). Improving the long-term outcome of DVT patients at risk for PTS will therefore ...
    • Tetrandrine identified in a small molecule screen to activate mesenchymal stem cells for enhanced immunomodulation 

      Yang, Zijiang; Concannon, John; Ng, Kelvin S.; Seyb, Kathleen; Mortensen, Luke J.; Ranganath, Sudhir; Gu, Fangqi; Levy, Oren; Tong, Zhixiang; Martyn, Keir; Zhao, Weian; Lin, Charles P.; Glicksman, Marcie A.; Karp, Jeffrey M. (Nature Publishing Group, 2016)
      Pre-treatment or priming of mesenchymal stem cells (MSC) prior to transplantation can significantly augment the immunosuppressive effect of MSC-based therapies. In this study, we screened a library of 1402 FDA-approved ...
    • Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy 

      Wang, Ke; Liu, Tzu-Ming; Wu, Juwell; Horton, Nicholas G.; Lin, Charles P.; Xu, Chris (Optical Society of America, 2012)
      We demonstrate a fiber-based, three-color femtosecond source for simultaneous imaging of three fluorescent proteins (FPs) using two-photon fluorescence microscopy (2PM). The three excitation wavelengths at 775 nm, 864 nm ...
    • Tracking Single Cells in Live Animals Using a Photoconvertible Near-Infrared Cell Membrane Label 

      Carlson, Alicia L.; Fujisaki, Joji; Wu, Juwell; Runnels, Judith M.; Turcotte, Raphaël; Celso, Cristina Lo; Scadden, David T.; Strom, Terry B.; Lin, Charles P. (Public Library of Science, 2013)
      We describe a novel photoconversion technique to track individual cells in vivo using a commercial lipophilic membrane dye, DiR. We show that DiR exhibits a permanent fluorescence emission shift (photoconversion) after ...
    • Two-Photon Antenna-Core Oxygen Probe with Enhanced Performance 

      Roussakis, Emmanuel; Spencer, Joel A.; Lin, Charles P.; Vinogradov, Sergei A. (American Chemical Society, 2014)
      Recent development of two-photon phosphorescence lifetime microscopy (2PLM) of oxygen enabled first noninvasive high-resolution measurements of tissue oxygenation in vivo in 3D, providing valuable physiological information. ...