Now showing items 1-18 of 18

    • CRISPR-mediated direct mutation of cancer genes in the mouse liver 

      Xue, Wen; Chen, Sidi; Yin, Hao; Tammela, Tuomas; Papagiannakopoulos, Thales; Joshi, Nikhil S.; Cai, Wenxin; Yang, Gillian; Bronson, Roderick; Crowley, Denise G.; Zhang, Feng; Anderson, Daniel G.; Sharp, Phillip A.; Jacks, Tyler (2014)
      The study of cancer genes in mouse models has traditionally relied on genetically-engineered strains made via transgenesis or gene targeting in embryonic stem (ES) cells1. Here we describe a new method of cancer model ...
    • A defined synthetic substrate for serum-free culture of human stem cell derived cardiomyocytes with improved functional maturity identified using combinatorial materials microarrays 

      Patel, Asha K.; Celiz, Adam D.; Rajamohan, Divya; Anderson, Daniel G.; Langer, Robert; Davies, Martyn C.; Alexander, Morgan R.; Denning, Chris (2016)
      Cardiomyocytes from human stem cells have applications in regenerative medicine and can provide models for heart disease and toxicity screening. Soluble components of the culture system such as growth factors within serum ...
    • Efficiency of siRNA delivery by lipid nanoparticles is limited by endocytic recycling 

      Sahay, Gaurav; Querbes, William; Alabi, Christopher; Eltoukhy, Ahmed; Sarkar, Sovan; Zurenko, Christopher; Karagiannis, Emannouil; Love, Kevin; Chen, Delai; Zoncu, Roberto; Buganim, Yosef; Schroeder, Avi; Langer, Robert; Anderson, Daniel G. (2013)
      Despite substantial efforts to understand the interactions between nanoparticles and cells, the cellular processes that determine the efficiency of intracellular drug delivery remain largely unclear. Here we examined ...
    • Genetic and hypoxic alterations of the microRNA-210-ISCU1/2 axis promote iron–sulfur deficiency and pulmonary hypertension 

      White, Kevin; Lu, Yu; Annis, Sofia; Hale, Andrew E; Chau, B Nelson; Dahlman, James E; Hemann, Craig; Opotowsky, Alexander R; Vargas, Sara O; Rosas, Ivan; Perrella, Mark A; Osorio, Juan C; Haley, Kathleen J; Graham, Brian B; Kumar, Rahul; Saggar, Rajan; Saggar, Rajeev; Wallace, W Dean; Ross, David J; Khan, Omar F; Bader, Andrew; Gochuico, Bernadette R; Matar, Majed; Polach, Kevin; Johannessen, Nicolai M; Prosser, Haydn M; Anderson, Daniel G; Langer, Robert; Zweier, Jay L; Bindoff, Laurence A; Systrom, David; Waxman, Aaron B; Jin, Richard C; Chan, Stephen Y (BlackWell Publishing Ltd, 2015)
      Iron–sulfur (Fe-S) clusters are essential for mitochondrial metabolism, but their regulation in pulmonary hypertension (PH) remains enigmatic. We demonstrate that alterations of the miR-210-ISCU1/2 axis cause Fe-S deficiencies ...
    • Long term Glycemic Control Using Polymer Encapsulated, Human Stem-Cell Derived β-cells in Immune Competent mice 

      Vegas, Arturo J.; Veiseh, Omid; Gürtler, Mads; Millman, Jeffrey R.; Pagliuca, Felicia W.; Bader, Andrew R.; Doloff, Joshua C.; Li, Jie; Chen, Michael; Olejnik, Karsten; Tam, Hok Hei; Jhunjhunwala, Siddharth; Langan, Erin; Aresta-Dasilva, Stephanie; Gandham, Srujan; McGarrigle, James; Bochenek, Matthew A.; Hollister-Lock, Jennifer; Oberholzer, Jose; Greiner, Dale L.; Weir, Gordon C.; Melton, Douglas A.; Langer, Robert; Anderson, Daniel G. (2016)
      The transplantation of glucose-responsive, insulin-producing cells offers the potential for restoring glycemic control in diabetic patients1. Pancreas transplantation and the infusion of cadaveric islets are currently ...
    • Loss of α-catenin elicits a cholestatic response and impairs liver regeneration 

      Herr, Keira Joann; Tsang, Ying-hung Nicole; Ong, Joanne Wei En; Li, Qiushi; Yap, Lai Lai; Yu, Weimiao; Yin, Hao; Bogorad, Roman L.; Dahlman, James E.; Chan, Yee Gek; Bay, Boon Huat; Singaraja, Roshni; Anderson, Daniel G.; Koteliansky, Victor; Viasnoff, Virgile; Thiery, Jean Paul (Nature Publishing Group, 2014)
      The liver is unique in its capacity to regenerate after injury, during which hepatocytes actively divide and establish cell-cell contacts through cell adhesion complexes. Here, we demonstrate that the loss of α-catenin, a ...
    • Macrophages retain hematopoietic stem cells in the spleen via VCAM-1 

      Dutta, Partha; Hoyer, Friedrich Felix; Grigoryeva, Lubov S.; Sager, Hendrik B.; Leuschner, Florian; Courties, Gabriel; Borodovsky, Anna; Novobrantseva, Tatiana; Ruda, Vera M.; Fitzgerald, Kevin; Iwamoto, Yoshiko; Wojtkiewicz, Gregory; Sun, Yuan; Da Silva, Nicolas; Libby, Peter; Anderson, Daniel G.; Swirski, Filip K.; Weissleder, Ralph; Nahrendorf, Matthias (The Rockefeller University Press, 2015)
      Splenic myelopoiesis provides a steady flow of leukocytes to inflamed tissues, and leukocytosis correlates with cardiovascular mortality. Yet regulation of hematopoietic stem cell (HSC) activity in the spleen is incompletely ...
    • Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells 

      Li, Wei; Lee, Samuel; Ma, Minglin; Kim, Soo Min; Guye, Patrick; Pancoast, James R.; Anderson, Daniel G.; Weiss, Ron; Lee, Richard T.; Hammond, Paula T. (Nature Publishing Group, 2013)
      Diabetes is caused by the loss or dysfunction of insulin-secreting β-cells in the pancreas. β-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular ...
    • Molecularly Self-Assembled Nucleic Acid Nanoparticles for Targeted In Vivo siRNA Delivery 

      Lee, Hyukjin; Lytton-Jean, Abigail K. R.; Chen, Yi; Love, Kevin T.; Park, Angela I.; Karagiannis, Emmanouil D.; Sehgal, Alfica; Querbes, William; Zurenko, Christopher S.; Jayaraman, Muthusamy; Peng, Chang G.; Charisse, Klaus; Borodovsky, Anna; Manoharan, Muthiah; Donahoe, Jessica S.; Truelove, Jessica; Nahrendorf, Matthias; Langer, Robert; Anderson, Daniel G. (2013)
      Nanoparticles are employed for delivering therapeutics into cells1,2. However, size, shape, surface chemistry and the presentation of targeting ligands on the surface of nanoparticles can affect circulation half-life and ...
    • Nanoparticle-formulated siRNA targeting integrins inhibits hepatocellular carcinoma progression in mice 

      Bogorad, Roman L; Yin, Hao; Zeigerer, Anja; Nonaka, Hidenori; Ruda, Vera; Zerial, Marino; Anderson, Daniel G; Koteliansky, Victor (2014)
      Integrins play an important role during development, regulating cell differentiation, proliferation and survival. Here we show that knockdown of integrin subunits slows down the progression of hepatocellular carcinoma ...
    • Neutrophil Responses to Sterile Implant Materials 

      Jhunjhunwala, Siddharth; Aresta-DaSilva, Stephanie; Tang, Katherine; Alvarez, David; Webber, Matthew J.; Tang, Benjamin C.; Lavin, Danya M.; Veiseh, Omid; Doloff, Joshua C.; Bose, Suman; Vegas, Arturo; Ma, Minglin; Sahay, Gaurav; Chiu, Alan; Bader, Andrew; Langan, Erin; Siebert, Sean; Li, Jie; Greiner, Dale L.; Newburger, Peter E.; von Andrian, Ulrich H.; Langer, Robert; Anderson, Daniel G. (Public Library of Science, 2015)
      In vivo implantation of sterile materials and devices results in a foreign body immune response leading to fibrosis of implanted material. Neutrophils, one of the first immune cells to be recruited to implantation sites, ...
    • Niemann-Pick C1 Affects the Gene Delivery Efficacy of Degradable Polymeric Nanoparticles 

      Eltoukhy, Ahmed A.; Sahay, Gaurav; Cunningham, James M.; Anderson, Daniel G. (American Chemical Society, 2014)
      Despite intensive research effort, the rational design of improved nanoparticulate drug carriers remains challenging, in part due to a limited understanding of the determinants of nanoparticle entry and transport in target ...
    • Precision cancer mouse models through genome editing with CRISPR-Cas9 

      Mou, Haiwei; Kennedy, Zachary; Anderson, Daniel G.; Yin, Hao; Xue, Wen (BioMed Central, 2015)
      The cancer genome is highly complex, with hundreds of point mutations, translocations, and chromosome gains and losses per tumor. To understand the effects of these alterations, precise models are needed. Traditional ...
    • Proliferation and Recruitment Contribute to Myocardial Macrophage Expansion in Chronic Heart Failure 

      Sager, Hendrik B.; Hulsmans, Maarten; Lavine, Kory J.; Moreira, Marina B.; Heidt, Timo; Courties, Gabriel P; Sun, Yuan; Iwamoto, Yoshiko; Tricot, Benoit; Khan, Omar F.; Dahlman, James E.; Borodovsky, Anna; Fitzgerald, Kevin; Anderson, Daniel Griffith; Weissleder, Ralph; Libby, Peter; Swirski, Filip K.; Nahrendorf, Matthias (Ovid Technologies (Wolters Kluwer Health), 2016)
      Rationale: Macrophages reside in the healthy myocardium, participate in ischemic heart disease, and modulate myocardial infarction (MI) healing. Their origin and roles in post-MI remodeling of nonischemic remote myocardium, ...
    • Size- and shape-dependent foreign body immune response to materials implanted in rodents and non-human primates 

      Veiseh, Omid; Doloff, Joshua C.; Ma, Minglin; Vegas, Arturo J.; Tam, Hok Hei; Bader, Andrew R.; Li, Jie; Langan, Erin; Wyckoff, Jeffrey; Loo, Whitney S.; Jhunjhunwala, Siddharth; Chiu, Alan; Siebert, Sean; Tang, Katherine; Hollister-Lock, Jennifer; Aresta-Dasilva, Stephanie; Bochenek, Matthew; Mendoza-Elias, Joshua; Wang, Yong; Qi, Merigeng; Lavin, Danya M.; Chen, Michael; Dholakia, Nimit; Thakrar, Raj; Lacík, Igor; Weir, Gordon C.; Oberholzer, Jose; Greiner, Dale L.; Langer, Robert; Anderson, Daniel G. (2015)
      The efficacy of implanted biomedical devices is often compromised by host recognition and subsequent foreign body responses. Here, we demonstrate the role of the geometry of implanted materials on their biocompatibility ...
    • Systemic RNAi-mediated Gene Silencing in Nonhuman Primate and Rodent Myeloid Cells 

      Novobrantseva, Tatiana I; Borodovsky, Anna; Wong, Jamie; Klebanov, Boris; Zafari, Mohammad; Yucius, Kristina; Querbes, William; Ge, Pei; Ruda, Vera M.; Milstein, Stuart; Speciner, Lauren; Duncan, Rick; Barros, Scott; Basha, Genc; Cullis, Pieter; Akinc, Akin; Donahoe, Jessica S; Narayanannair Jayaprakash, K; Jayaraman, Muthusamy; Bogorad, Roman L; Love, Kevin; Whitehead, Katie; Levins, Chris; Manoharan, Muthiah; Swirski, Filip K.; Weissleder, Ralph; Langer, Robert S.; Anderson, Daniel Griffith; de Fougerolles, Antonin; Nahrendorf, Matthias; Koteliansky, Victor (Nature Publishing Group, 2012)
      Leukocytes are central regulators of inflammation and the target cells of therapies for key diseases, including autoimmune, cardiovascular, and malignant disorders. Efficient in vivo delivery of small interfering RNA (siRNA) ...
    • A versatile reporter system for CRISPR-mediated chromosomal rearrangements 

      Li, Yingxiang; Park, Angela I.; Mou, Haiwei; Colpan, Cansu; Bizhanova, Aizhan; Akama-Garren, Elliot; Joshi, Nik; Hendrickson, Eric A.; Feldser, David; Yin, Hao; Anderson, Daniel G.; Jacks, Tyler; Weng, Zhiping; Xue, Wen (BioMed Central, 2015)
      Although chromosomal deletions and inversions are important in cancer, conventional methods for detecting DNA rearrangements require laborious indirect assays. Here we develop fluorescent reporters to rapidly quantify ...
    • YY1 Regulates Melanocyte Development and Function by Cooperating with MITF 

      Li, Juying; Bell, Robert J. A.; Liu, Huifei; Love, Kevin T.; Larue, Lionel; Song, Jun S.; Tran, Thanh-Nga Trinh; Haq, Rizwan; Langer, Robert S.; Anderson, Daniel Griffith; Fisher, David Erich (Public Library of Science, 2012)
      Studies of coat color mutants have greatly contributed to the discovery of genes that regulate melanocyte development and function. Here, we generated Yy1 conditional knockout mice in the melanocyte-lineage and observed ...