Browsing by Author "Blower, Michael"
Now showing items 1-7 of 7
-
The calcium-dependent ribonuclease XendoU promotes ER network formation through local RNA degradation
Schwarz, Dianne S.; Blower, Michael D. (The Rockefeller University Press, 2014)How cells shape and remodel organelles in response to cellular signals is a poorly understood process. Using Xenopus laevis egg extract, we found that increases in cytosolic calcium lead to the activation of an endogenous ... -
Combining Different mRNA Capture Methods to Analyze the Transcriptome: Analysis of the Xenopus laevis Transcriptome
Blower, Michael D.; Jambhekar, Ashwini; Schwarz, Dianne S.; Toombs, James A. (Public Library of Science, 2013)mRNA sequencing (mRNA-seq) is a commonly used technique to survey gene expression from organisms with fully sequenced genomes. Successful mRNA-seq requires purification of mRNA away from the much more abundant ribosomal ... -
The endoplasmic reticulum: structure, function and response to cellular signaling
Schwarz, Dianne S.; Blower, Michael D. (Springer International Publishing, 2015)The endoplasmic reticulum (ER) is a large, dynamic structure that serves many roles in the cell including calcium storage, protein synthesis and lipid metabolism. The diverse functions of the ER are performed by distinct ... -
ESCRT-II controls retinal axon growth by regulating DCC receptor levels and local protein synthesis
Konopacki, Filip A.; Wong, Hovy Ho-Wai; Dwivedy, Asha; Bellon, Anaïs; Blower, Michael D.; Holt, Christine E. (The Royal Society, 2016)Endocytosis and local protein synthesis (LPS) act coordinately to mediate the chemotropic responses of axons, but the link between these two processes is poorly understood. The endosomal sorting complex required for transport ... -
The MAP kinase pathway coordinates crossover designation with disassembly of synaptonemal complex proteins during meiosis
Nadarajan, Saravanapriah; Mohideen, Firaz; Tzur, Yonatan B; Ferrandiz, Nuria; Crawley, Oliver; Montoya, Alex; Faull, Peter; Snijders, Ambrosius P; Cutillas, Pedro R; Jambhekar, Ashwini; Blower, Michael D; Martinez-Perez, Enrique; Harper, J Wade; Colaiacovo, Monica P (eLife Sciences Publications, Ltd, 2016)Asymmetric disassembly of the synaptonemal complex (SC) is crucial for proper meiotic chromosome segregation. However, the signaling mechanisms that directly regulate this process are poorly understood. Here we show that ... -
Molecular Details and Functional Analysis of RNA Binding by ESCRT-II
Emerman, Amy Beth (2015-05-18)Many RNAs show distinct localization patterns in cells with enrichment at particular subcellular sites or organelles. RNA localization is a highly conserved process that both spatially and temporally controls gene expression. ... -
RNA Stimulates Aurora B Kinase Activity during Mitosis
Jambhekar, Ashwini; Emerman, Amy B.; Schweidenback, Caterina T. H.; Blower, Michael D. (Public Library of Science, 2014)Accurate chromosome segregation is essential for cell viability. The mitotic spindle is crucial for chromosome segregation, but much remains unknown about factors that regulate spindle assembly. Recent work implicates RNA ...