Browsing by Author "Dartt, Darlene"
Now showing items 1-10 of 10
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Artificial Polymeric Scaffolds as Extracellular Matrix Substitutes for Autologous Conjunctival Goblet Cell Expansion
He, Min; Storr-Paulsen, Thomas; Wang, Annie L.; Ghezzi, Chiara E.; Wang, Siran; Fullana, Matthew; Karamichos, Dimitrios; Utheim, Tor P.; Islam, Rakibul; Griffith, May; Islam, M. Mirazul; Hodges, Robin R.; Wnek, Gary E.; Kaplan, David L.; Dartt, Darlene A. (The Association for Research in Vision and Ophthalmology, 2016)Purpose We fabricated and investigated polymeric scaffolds that can substitute for the conjunctival extracellular matrix to provide a substrate for autologous expansion of human conjunctival goblet cells in culture. Methods: ... -
Concise Review: Comparison of Culture Membranes Used for Tissue Engineered Conjunctival Epithelial Equivalents
Eidet, Jon Roger; Dartt, Darlene A.; Utheim, Tor Paaske (MDPI, 2015)The conjunctival epithelium plays an important role in ensuring the optical clarity of the cornea by providing lubrication to maintain a smooth, refractive surface, by producing mucins critical for tear film stability and ... -
Dry Eye Disease Patients with Xerostomia Report Higher Symptom Load and Have Poorer Meibum Expressibility
Fostad, Ida G.; Eidet, Jon R.; Utheim, Tor P.; Ræder, Sten; Lagali, Neil S.; Messelt, Edvard B.; Dartt, Darlene A. (Public Library of Science, 2016)The purpose of the study was to investigate if xerostomia (dry mouth) is associated with symptoms and signs of dry eye disease (DED). At the Norwegian Dry Eye Clinic, patients with symptomatic DED with different etiologies ... -
Effect of Storage Temperature on Structure and Function of Cultured Human Oral Keratinocytes
Islam, Rakibul; Jackson, Catherine; Eidet, Jon R.; Messelt, Edward B.; Corraya, Rima Maria; Lyberg, Torstein; Griffith, May; Dartt, Darlene A.; Utheim, Tor P. (Public Library of Science, 2015)Purpose/Aims To assess the effect of storage temperature on the viability, phenotype, metabolism, and morphology of cultured human oral keratinocytes (HOK). Materials and Methods Cultured HOK cells were stored in HEPES- ... -
Lipoxin A4 activates ALX/FPR2 Receptor to Regulate Conjunctival Goblet Cell Secretion
Hodges, Robin R.; Li, Dayu; Shatos, Marie A.; Bair, Jeffrey A.; Lippestad, Marit; Serhan, Charles N.; Dartt, Darlene A. (2016)Conjunctival goblet cells play a major role in maintaining the mucous layer of the tear film under physiological conditions as well as in inflammatory diseases like dry eye and allergic conjunctivitis.. Resolution of ... -
Lipoxin A4 Counter-regulates Histamine-stimulated Glycoconjugate Secretion in Conjunctival Goblet Cells
Hodges, Robin R.; Li, Dayu; Shatos, Marie A.; Serhan, Charles N.; Dartt, Darlene A. (Nature Publishing Group, 2016)Conjunctival goblet cells synthesize and secrete mucins which play an important role in protecting the ocular surface. Pro-resolution mediators, such as lipoxin A4 (LXA4), are produced during inflammation returning the ... -
Resolvin D1 and Aspirin-Triggered Resolvin D1 Regulate Histamine-stimulated Conjunctival Goblet Cell Secretion
Li, Dayu; Hodges, Robin R.; Jiao, Jianwei; Carozza, Richard B.; Shatos, Marie A.; Chiang, Nan; Serhan, Charles N.; Dartt, Darlene A. (2013)Resolution of inflammation is an active process mediated by pro-resolution lipid mediators. Since resolvin (Rv) D1 is produced in the cornea, pro-resolution mediators could be effective in regulating inflammatory responses ... -
Serum-Free and Xenobiotic-Free Preservation of Cultured Human Limbal Epithelial Cells
Utheim, Oeygunn; Islam, Rakibul; Lyberg, Torstein; Roald, Borghild; Eidet, Jon Roger; de la Paz, Maria Fideliz; Dartt, Darlene A.; Raeder, Sten; Utheim, Tor Paaske (Public Library of Science, 2015)Aim/Purpose of the Study To develop a one-week storage method, without serum and xenobiotics, that would maintain cell viability, morphology, and phenotype of cultured human limbal epithelial sheets. Materials and Methods ... -
Staphylococcus aureus Activates the NLRP3 Inflammasome in Human and Rat Conjunctival Goblet Cells
McGilligan, Victoria E.; Gregory-Ksander, Meredith S.; Li, Dayu; Moore, Jonathan E.; Hodges, Robin R.; Gilmore, Michael S.; Moore, Tara C. B.; Dartt, Darlene A. (Public Library of Science, 2013)The conjunctiva is a moist mucosal membrane that is constantly exposed to an array of potential pathogens and triggers of inflammation. The NACHT, leucine rich repeat (LRR), and pyrin domain-containing protein 3 (NLRP3) ... -
Storage Temperature Alters the Expression of Differentiation-Related Genes in Cultured Oral Keratinocytes
Utheim, Tor Paaske; Islam, Rakibul; Fostad, Ida G.; Eidet, Jon R.; Sehic, Amer; Olstad, Ole K.; Dartt, Darlene A.; Messelt, Edward B.; Griffith, May; Pasovic, Lara (Public Library of Science, 2016)Purpose Storage of cultured human oral keratinocytes (HOK) allows for transportation of cultured transplants to eye clinics worldwide. In a previous study, one-week storage of cultured HOK was found to be superior with ...