Browsing by Author "Lee, Ming-Chun"
Now showing items 1-5 of 5
-
Development of an Optimized Medium, Strain and High-Throughput Culturing Methods for Methylobacterium extorquens
Delaney, Nigel Francis; Kaczmarek, Maria E.; Ward, Lewis M.; Swanson, Paige Kathleen; Lee, Ming-Chun; Marx, Christopher J (Public Library of Science, 2013)Methylobacterium extorquens strains are the best-studied methylotrophic model system, and their metabolism of single carbon compounds has been studied for over 50 years. Here we develop a new system for high-throughput ... -
FREQ-Seq: A Rapid, Cost-Effective, Sequencing-Based Method to Determine Allele Frequencies Directly from Mixed Populations
Chubiz, Lon M; Lee, Ming-Chun; Delaney, Nigel Francis; Marx, Christopher J (Public Library of Science, 2012)Understanding evolutionary dynamics within microbial populations requires the ability to accurately follow allele frequencies through time. Here we present a rapid, cost-effective method (FREQ-Seq) that leverages Illumina ... -
Methylobacterium Genome Sequences: A Reference Blueprint to Investigate Microbial Metabolism of C1 Compounds from Natural and Industrial Sources
Vuilleumier, Stéphane; Chistoserdova, Ludmila; Bringel, Françoise; Lajus, Aurélie; Gourion, Benjamin; Barbe, Valérie; Chang, Jean; Cruveiller, Stéphane; Dossat, Carole; Gillett, Will; Gruffaz, Christelle; Haugen, Eric; Hourcade, Edith; Levy, Ruth; Mangenot, Sophie; Muller, Emilie; Nadalig, Thierry; Pagni, Marco; Penny, Christian; Peyraud, Rémi; Roche, David; Rouy, Zoé; Saenampechek, Channakhone; Salvignol, Grégory; Vallenet, David; Wu, Zaining; Vorholt, Julia A.; Olson, Maynard V.; Kaul, Rajinder; Weissenbach, Jean; Médigue, Claudine; Lidstrom, Mary E.; Lee, Ming-Chun; Zhou, Yang; Robinson, David G.; Marx, Christopher J (Public Library of Science, 2009)Methylotrophy describes the ability of organisms to grow on reduced organic compounds without carbon-carbon bonds. The genomes of two pink-pigmented facultative methylotrophic bacteria of the Alpha-proteobacterial genus ... -
OASIS: an automated program for global investigation of bacterial and archaeal insertion sequences
Robinson, David G.; Lee, Ming-Chun; Marx, Christopher J (Oxford University Press, 2012)Insertion sequences (ISs) are simple transposable elements present in most bacterial and archaeal genomes and play an important role in genomic evolution. The recent expansion of sequenced genomes offers the opportunity ... -
Repeated, Selection-Driven Genome Reduction of Accessory Genes in Experimental Populations
Lee, Ming-Chun; Marx, Christopher J (Public Library of Science, 2012)Genome reduction has been observed in many bacterial lineages that have adapted to specialized environments. The extreme genome degradation seen for obligate pathogens and symbionts appears to be dominated by genetic drift. ...