Browsing by Author "Gaudet, Rachelle"

Now showing items 1-20 of 33

    • Antigen Processing and Presentation: TAPping into ABC Transporters 

      Procko, Erik; Gaudet, Rachelle (Elsevier, 2009)
      Adaptive, cell-mediated immunity involves the presentation of antigenic peptides on class I MHC molecules at the cell surface. This requires an ABC transporter associated with antigen processing (TAP) to transport antigenic ...

    • Antiparallel protocadherin homodimers use distinct affinity- and specificity-mediating regions in cadherin repeats 1-4 

      Nicoludis, John M; Vogt, Bennett E; Green, Anna G; Schärfe, Charlotta PI; Marks, Debora S; Gaudet, Rachelle (eLife Sciences Publications, Ltd, 2016)
      Protocadherins (Pcdhs) are cell adhesion and signaling proteins used by neurons to develop and maintain neuronal networks, relying on trans homophilic interactions between their extracellular cadherin (EC) repeat domains. ...

    • Applications of sequence coevolution in membrane protein biochemistry 

      Nicoludis, John M.; Gaudet, Rachelle (Elsevier BV, 2018-04)
      Recently, protein sequence coevolution analysis has matured into a predictive powerhouse for protein structure and function. Direct methods, which use global statistical models of sequence coevolution, have enabled the ...

    • Batrachotoxin acts as a stent to hold open homotetrameric prokaryotic voltage-gated sodium channels 

      Finol-Urdaneta, Rocio K.; McArthur, Jeffrey R.; Goldschen-Ohm, Marcel P.; Gaudet, Rachelle; Tikhonov, Denis B.; Zhorov, Boris S.; French, Robert J.; McArthur (Rockefeller University Press, 2018-12-26)
      Batrachotoxin (BTX), an alkaloid from skin secretions of dendrobatid frogs, causes paralysis and death by facilitating activation and inhibiting deactivation of eukaryotic voltage-gated sodium (Nav) channels, which underlie ...

    • Biochemical and Crystallographic Studies of the Escherichia Coli β-Barrel Assembly Machine 

      Westwood, David (2016-09-01)
      Bacteria have developed resistance mechanisms to every class of antibiotics that has been created and combating the rise of multidrug-resistant bacteria—a major threat to public health—requires the continual development ...

    • Characterization and Structural Studies of the Plasmodium Falciparum Ubiquitin and Nedd8 Hydrolase UCHL3 

      Artavanis-Tsakonas, Katerina; Weihofen, Wilhelm Andreas; Antos, John M.; Coleman, Bradley Ian; Comeaux, Christy Ann; Duraisingh, Manoj T.; Gaudet, Rachelle; Ploegh, Hidde L. (American Society for Biochemistry and Molecular Biology, 2010)
      Like their human hosts, Plasmodium falciparum parasites rely on the ubiquitin-proteasome system for survival. We previously identified PfUCHL3, a deubiquitinating enzyme, and here we characterize its activity and changes ...

    • Crystal Structure and Conformational Change Mechanism of a Bacterial Nramp-Family Divalent Metal Transporter 

      Bozzi, Aaron Thomas; Bane, Lukas Blackford; Weihofen, Wilhelm A.; Singharoy, Abhishek; Guillen, Eduardo R.; Ploegh, Hidde L.; Schulten, Klaus; Gaudet, Rachelle (Elsevier BV, 2016)
      The widely-conserved natural resistance associated macrophage protein (Nramp) family of divalent metal transporters enables manganese import in bacteria and dietary iron uptake in mammals. We determined the crystal structure ...

    • D-helix influences dimerization of the ATP-binding cassette (ABC) transporter associated with antigen processing 1 (TAP1) nucleotide-binding domain 

      Vakkasoglu, Ahmet; Srikant, Sriram; Gaudet, Rachelle (Public Library of Science (PLoS), 2017)
      ATP-binding cassette (ABC) transporters form a large family of transmembrane importers and exporters. Using two nucleotide-binding domains (NBDs), which form a canonical ATP-sandwich dimer at some point within the transport ...

    • Data publication with the structural biology data grid supports live analysis 

      Meyer, Peter A.; Socias, Stephanie; Key, Jason; Ransey, Elizabeth; Tjon, Emily C.; Buschiazzo, Alejandro; Lei, Ming; Botka, Chris; Withrow, James; Neau, David; Rajashankar, Kanagalaghatta; Anderson, Karen S.; Baxter, Richard H.; Blacklow, Stephen C.; Boggon, Titus J.; Bonvin, Alexandre M. J. J.; Borek, Dominika; Brett, Tom J.; Caflisch, Amedeo; Chang, Chung-I; Chazin, Walter J.; Corbett, Kevin D.; Cosgrove, Michael S.; Crosson, Sean; Dhe-Paganon, Sirano; Di Cera, Enrico; Drennan, Catherine L.; Eck, Michael J.; Eichman, Brandt F.; Fan, Qing R.; Ferré-D'Amaré, Adrian R.; Christopher Fromme, J.; Garcia, K. Christopher; Gaudet, Rachelle; Gong, Peng; Harrison, Stephen C.; Heldwein, Ekaterina E.; Jia, Zongchao; Keenan, Robert J.; Kruse, Andrew C.; Kvansakul, Marc; McLellan, Jason S.; Modis, Yorgo; Nam, Yunsun; Otwinowski, Zbyszek; Pai, Emil F.; Pereira, Pedro José Barbosa; Petosa, Carlo; Raman, C. S.; Rapoport, Tom A.; Roll-Mecak, Antonina; Rosen, Michael K.; Rudenko, Gabby; Schlessinger, Joseph; Schwartz, Thomas U.; Shamoo, Yousif; Sondermann, Holger; Tao, Yizhi J.; Tolia, Niraj H.; Tsodikov, Oleg V.; Westover, Kenneth D.; Wu, Hao; Foster, Ian; Fraser, James S.; Maia, Filipe R. N C.; Gonen, Tamir; Kirchhausen, Tom; Diederichs, Kay; Crosas, Mercè; Sliz, Piotr (Nature Publishing Group, 2016)
      Access to experimental X-ray diffraction image data is fundamental for validation and reproduction of macromolecular models and indispensable for development of structural biology processing methods. Here, we established ...

    • Discovery of Small-Molecule Autophagy Modulators in Mammalian Systems 

      Kuo, Szu-Yu (2016-06-07)
      Autophagy is an evolutionarily conserved catabolic process in eukaryotes, which involves the formation of double-membrane vesicles that enclose cellular components and fuse with lysosomes. Autophagy is critical to the ...

    • Distinct Properties of \(Ca^{2+}\)-Calmodulin Binding to N- and C-Terminal Regulatory Regions of the TRPV1 Channel 

      Lau, Sze-Yi; Procko, Erik; Gaudet, Rachelle (Rockefeller University Press, 2012)
      Transient receptor potential (TRP) vanilloid 1 (TRPV1) is a molecular pain receptor belonging to the TRP superfamily of nonselective cation channels. As a polymodal receptor, TRPV1 responds to heat and a wide range of ...

    • Divide and Conquer: High Resolution Structural Information on TRP Channel Fragments 

      Gaudet, Rachelle (Rockefeller University Press, 2009)
      Understanding how proteins facilitate signaling and substrate transport across biological membranes is an important frontier of structural biology. Membrane proteins are the doors and windows of cells: many membrane proteins ...

    • Exome Sequencing Identifies a Novel TRPV4 Mutation in a CMT2C Family 

      Gaudet, Rachelle; Landouré, Guida; Sullivan, Jeremy M.; Johnson, Janel O.; Munns, Clare H.; Shi, Yijun; Diallo, Oumarou; Ludlow, Christy L.; Fischbeck, Kenneth H.; Traynor, Bryan J.; Burnett, Barrington G.; Sumner, Charlotte J.; Gibbs, Raphael J. (American Academy of Neurology, 2013-07-05)

    • Functionally Important Interactions between the Nucleotide-Binding Domains of an Antigenic Peptide Transporter 

      Procko, Erik; Gaudet, Rachelle (American Chemical Society, 2008)
      The transporter associated with antigen processing (TAP), an ABC transporter, pumps cytosolic peptides into the endoplasmic reticulum, where the peptides are loaded onto class I MHC molecules for presentation to the immune ...

    • High-Affinity Alkynyl Bisubstrate Inhibitors of NicotinamideN-Methyltransferase (NNMT) 

      Policarpo, Rocco; Decultot, Ludovic; May, Elizabeth; Kuzmič, Petr; Carlson, Samuel; Huang, Danny; Chu, Vincent; Wright, Brandon; Dhakshinamoorthy, Saravanakumar; Kannt, Aimo; Rani, Shilpa; Dittakavi, Sreekanth; Panarese, Joseph; Gaudet, Rachelle; Shair, Matthew (American Chemical Society (ACS), 2019-10-07)
      Nicotinamide N-methyltransferase (NNMT) is a metabolic enzyme that methylates nicotinamide (NAM) using cofactor S-adenosylmethionine (SAM). NNMT overexpression has been linked to diabetes, obesity, and various cancers. In ...

    • Mechanistic Determinants of the Directionality and Energetics of Active Export by a Heterodimeric ABC Transporter 

      Grossmann, Nina; Vakkasoglu, Ahmet Selim; Hulpke, Sabine; Abele, Rupert; Gaudet, Rachelle; Tampé, Robert (Nature Publishing Group, 2014)
      The ATP-binding cassette (ABC) transporter associated with antigen processing (TAP) participates in immune surveillance by moving proteasomal products into the endoplasmic reticulum (ER) lumen for major histocompatibility ...

    • A mutational analysis of the Bacillus subtilis competence helicase ComFA 

      Chilton, Scott S. (2014-06-06)
      Genetic competence is a developmental process in bacteria that allows natural transformation. Competent Gram positive bacteria such as Bacillus subtilis carry a cytosolic helicase which is required for efficient transformation. ...

    • Novel mutations highlight the key role of the ankyrin repeat domain in TRPV4-mediated neuropathy 

      Sullivan, Jeremy M.; Zimanyi, Christina M.; Aisenberg, William; Bears, Breanne; Chen, Dong-Hui; Day, John W.; Bird, Thomas D.; Siskind, Carly E.; Gaudet, Rachelle; Sumner, Charlotte J. (Wolters Kluwer, 2015)
      Objective: To characterize 2 novel TRPV4 mutations in 2 unrelated families exhibiting the Charcot-Marie-Tooth disease type 2C (CMT2C) phenotype. Methods: Direct CMT gene testing was performed on 2 unrelated families with ...

    • A Partial Calcium-Free Linker Confers Flexibility to Inner-Ear Protocadherin-15 

      Powers, Robert E.; Gaudet, Rachelle; Sotomayor, Marcos (Elsevier BV, 2017)
      Tip links of the inner ear are protein filaments essential for hearing and balance. Two atypical cadherins, cadherin-23 and protocadherin-15, interact in a Ca2+-dependent manner to form tip links. The largely unknown ...

    • Phosphatidylinositol-4,5-Biphosphate-Dependent Rearrangement of TRPV4 Cytosolic Tails Enables Channel Activation by Physiological Stimuli 

      Garcia-Elias, Anna; Mrkonjic, Sanela; Pardo-Pastor, Carlos; Inada, Hitoshi; Hellmich, Ute; Rubio-Moscardó, Fanny; Plata, Cristina; Gaudet, Rachelle; Vicente, Rubén; Valverde, Miguel A. (National Academy of Sciences, 2013)
      Most transient receptor potential (TRP) channels are regulated by phosphatidylinositol-4,5-biphosphate (PIP\(_2\)), although the structural rearrangements occurring on PIP\(_2\) binding are currently far from clear. Here ...