Now showing items 1-20 of 29

    • AAV-expressed eCD4-Ig provides durable protection from multiple SHIV challenges 

      Gardner, Matthew R.; Kattenhorn, Lisa M.; Kondur, Hema R.; von Schaewen, Markus; Dorfman, Tatyana; Chiang, Jessica J.; Haworth, Kevin G.; Decker, Julie M.; Alpert, Michael D.; Bailey, Charles C.; Neale, Ernest S.; Fellinger, Christoph H.; Joshi, Vinita R.; Fuchs, Sebastian P.; Martinez-Navio, Jose M.; Quinlan, Brian D.; Yao, Annie Y.; Mouquet, Hugo; Gorman, Jason; Zhang, Baoshan; Poignard, Pascal; Nussenzweig, Michel C.; Burton, Dennis R.; Kwong, Peter D.; Piatak, Michael; Lifson, Jeffrey D.; Gao, Guangping; Desrosiers, Ronald C.; Evans, David T.; Hahn, Beatrice H.; Ploss, Alexander; Cannon, Paula M.; Seaman, Michael S.; Farzan, Michael (2015)
      Long-term in vivo expression of a broad and potent entry inhibitor could circumvent the need for a conventional vaccine for HIV-1. Adeno-associated virus (AAV) vectors can stably express HIV-1 broadly neutralizing antibodies ...
    • Anti-V3 Monoclonal Antibodies Display Broad Neutralizing Activities against Multiple HIV-1 Subtypes 

      Hioe, Catarina E.; Wrin, Terri; Seaman, Michael S.; Yu, Xuesong; Wood, Blake; Self, Steve; Williams, Constance; Gorny, Miroslaw K.; Zolla-Pazner, Susan (Public Library of Science, 2010)
      Background: The V3 loop of the HIV-1 envelope (Env) glycoprotein gp120 was identified as the “principal neutralizing domain” of HIV-1, but has been considered too variable to serve as a neutralizing antibody (Ab) target. ...
    • Antibody Responses After Analytic Treatment Interruption in Human Immunodeficiency Virus-1-Infected Individuals on Early Initiated Antiretroviral Therapy 

      Stephenson, Kathryn E.; Neubauer, George H.; Bricault, Christine A.; Shields, Jennifer; Bayne, Madeleine; Reimer, Ulf; Pawlowski, Nikolaus; Knaute, Tobias; Zerweck, Johannes; Seaman, Michael S.; Rosenberg, Eric S.; Barouch, Dan H. (Oxford University Press, 2016)
      The examination of antibody responses in human immunodeficiency virus (HIV)-1-infected individuals in the setting of antiretroviral treatment (ART) interruption can provide insight into the evolution of antibody responses ...
    • Antibody Responses after Intravaginal Immunisation with Trimeric HIV-1CN54 clade C gp140 in Carbopol Gel are Augmented by Systemic Priming or Boosting with an Adjuvanted Formulation 

      Cranage, Martin P.; Fraser, Carol A.; Cope, Alethea; McKay, Paul F.; Cole, Tom; Mahmoud, A. Nasir; Hall, Joanna; Giles, Elaine; Voss, Gerald; Page, Mark; Almond, Neil; Shattock, Robin J.; Seaman, Michael S. (Elsevier Science, 2011)
      Optimum strategies to elicit and maintain antibodies at mucosal portals of virus entry are critical for the development of vaccines against human immunodeficiency virus (HIV). Here we show in non-human primates that a novel ...
    • Broad Neutralization by a Combination of Antibodies Recognizing the CD4 Binding Site and a New Conformational Epitope on the HIV-1 Envelope Protein 

      Klein, Florian; Gaebler, Christian; Mouquet, Hugo; Sather, D. Noah; Lehmann, Clara; Scheid, Johannes F.; Kraft, Zane; Liu, Yan; Pietzsch, John; Hurley, Arlene; Poignard, Pascal; Feizi, Ten; Morris, Lynn; Walker, Bruce David; Fätkenheuer, Gerd; Seaman, Michael S.; Stamatatos, Leonidas; Nussenzweig, Michel C. (The Rockefeller University Press, 2012)
      Two to three years after infection, a fraction of HIV-1–infected individuals develop serologic activity that neutralizes most viral isolates. Broadly neutralizing antibodies that recognize the HIV-1 envelope protein have ...
    • Computational Prediction of Broadly Neutralizing HIV-1 Antibody Epitopes from Neutralization Activity Data 

      Ferguson, Andrew L.; Falkowska, Emilia; Walker, Laura M.; Seaman, Michael S.; Burton, Dennis R.; Chakraborty, Arup K. (Public Library of Science, 2013)
      Broadly neutralizing monoclonal antibodies effective against the majority of circulating isolates of HIV-1 have been isolated from a small number of infected individuals. Definition of the conformational epitopes on the ...
    • Different Pattern of Immunoglobulin Gene Usage by HIV-1 Compared to Non-HIV-1 Antibodies Derived from the Same Infected Subject 

      Li, Liuzhe; Wang, Xiao-Hong; Banerjee, Sagarika; Volsky, Barbara; Williams, Constance; Virland, Diana; Nadas, Arthur; Seaman, Michael S.; Chen, Xuemin; Spearman, Paul; Zolla-Pazner, Susan; Gorny, Miroslaw K. (Public Library of Science, 2012)
      A biased usage of immunoglobulin (Ig) genes is observed in human anti-HIV-1 monoclonal antibodies (mAbs) resulting probably from compensation to reduced usage of the VH3 family genes, while the other alternative suggests ...
    • The Effects of Somatic Hypermutation on Neutralization and Binding in the PGT121 Family of Broadly Neutralizing HIV Antibodies 

      Sok, Devin; Laserson, Uri; Laserson, Jonathan; Liu, Yi; Vigneault, Francois; Julien, Jean-Philippe; Briney, Bryan; Ramos, Alejandra; Saye, Karen F.; Le, Khoa; Mahan, Alison; Wang, Shenshen; Kardar, Mehran; Yaari, Gur; Walker, Laura M.; Simen, Birgitte B.; St. John, Elizabeth P.; Chan-Hui, Po-Ying; Swiderek, Kristine; Kleinstein, Stephen H.; Alter, Galit; Seaman, Michael S.; Chakraborty, Arup K.; Koller, Daphne; Wilson, Ian A.; Church, George M.; Burton, Dennis R.; Poignard, Pascal (Public Library of Science, 2013)
      Broadly neutralizing HIV antibodies (bnAbs) are typically highly somatically mutated, raising doubts as to whether they can be elicited by vaccination. We used 454 sequencing and designed a novel phylogenetic method to ...
    • Enhanced HIV-1 immunotherapy by commonly arising antibodies that target virus escape variants 

      Klein, Florian; Nogueira, Lilian; Nishimura, Yoshiaki; Phad, Ganesh; West, Anthony P.; Halper-Stromberg, Ariel; Horwitz, Joshua A.; Gazumyan, Anna; Liu, Cassie; Eisenreich, Thomas R.; Lehmann, Clara; Fätkenheuer, Gerd; Williams, Constance; Shingai, Masashi; Martin, Malcolm A.; Bjorkman, Pamela J.; Seaman, Michael S.; Zolla-Pazner, Susan; Karlsson Hedestam, Gunilla B.; Nussenzweig, Michel C. (The Rockefeller University Press, 2014)
      Antibody-mediated immunotherapy is effective in humanized mice when combinations of broadly neutralizing antibodies (bNAbs) are used that target nonoverlapping sites on the human immunodeficiency virus type 1 (HIV-1) ...
    • Features of Recently Transmitted HIV-1 Clade C Viruses that Impact Antibody Recognition: Implications for Active and Passive Immunization 

      Rademeyer, Cecilia; Korber, Bette; Seaman, Michael S.; Giorgi, Elena E.; Thebus, Ruwayhida; Robles, Alexander; Sheward, Daniel J.; Wagh, Kshitij; Garrity, Jetta; Carey, Brittany R.; Gao, Hongmei; Greene, Kelli M.; Tang, Haili; Bandawe, Gama P.; Marais, Jinny C.; Diphoko, Thabo E.; Hraber, Peter; Tumba, Nancy; Moore, Penny L.; Gray, Glenda E.; Kublin, James; McElrath, M. Juliana; Vermeulen, Marion; Middelkoop, Keren; Bekker, Linda-Gail; Hoelscher, Michael; Maboko, Leonard; Makhema, Joseph; Robb, Merlin L.; Abdool Karim, Salim; Abdool Karim, Quarraisha; Kim, Jerome H.; Hahn, Beatrice H.; Gao, Feng; Swanstrom, Ronald; Morris, Lynn; Montefiori, David C.; Williamson, Carolyn (Public Library of Science, 2016)
      The development of biomedical interventions to reduce acquisition of HIV-1 infection remains a global priority, however their potential effectiveness is challenged by very high HIV-1 envelope diversity. Two large prophylactic ...
    • A gp41 MPER-specific Llama VHH Requires a Hydrophobic CDR3 for Neutralization but not for Antigen Recognition 

      Lutje Hulsik, David; Liu, Ying-ying; Strokappe, Nika M.; Battella, Simone; El Khattabi, Mohamed; McCoy, Laura E.; Sabin, Charles; Hinz, Andreas; Hock, Miriam; Macheboeuf, Pauline; Bonvin, Alexandre M. J. J.; Langedijk, Johannes P. M.; Davis, David; Forsman Quigley, Anna; Aasa-Chapman, Marlén M. I.; Seaman, Michael S.; Ramos, Alejandra; Poignard, Pascal; Favier, Adrien; Simorre, Jean-Pierre; Weiss, Robin A.; Verrips, C. Theo; Weissenhorn, Winfried; Rutten, Lucy (Public Library of Science, 2013)
      The membrane proximal external region (MPER) of the HIV-1 glycoprotein gp41 is targeted by the broadly neutralizing antibodies 2F5 and 4E10. To date, no immunization regimen in animals or humans has produced HIV-1 neutralizing ...
    • HIV-1 Envelope Trimer Elicits Higher Neutralizing Antibody Responses than Monomeric Gp120 

      Kovacs, James Monroe; Nkolola, Joseph Patrick; Peng, H; Cheung, A; Perry, J; Miller, CA; Seaman, Michael S.; Barouch, Dan Hung; Chen, Bing (BioMed Central, 2012)
    • Identification of broadly neutralizing antibody epitopes in the HIV-1 envelope glycoprotein using evolutionary models 

      Lacerda, Miguel; Moore, Penny L; Ngandu, Nobubelo K; Seaman, Michael; Gray, Elin S; Murrell, Ben; Krishnamoorthy, Mohan; Nonyane, Molati; Madiga, Maphuti; Wibmer, Constantinos Kurt; Sheward, Daniel; Bailer, Robert T; Gao, Hongmei; Greene, Kelli M; Karim, Salim S Abdool; Mascola, John R; Korber, Bette TM; Montefiori, David C; Morris, Lynn; Williamson, Carolyn; Seoighe, Cathal (BioMed Central, 2013)
      Background: Identification of the epitopes targeted by antibodies that can neutralize diverse HIV-1 strains can provide important clues for the design of a preventative vaccine. Methods: We have developed a computational ...
    • Incomplete Neutralization and Deviation from Sigmoidal Neutralization Curves for HIV Broadly Neutralizing Monoclonal Antibodies 

      McCoy, Laura E.; Falkowska, Emilia; Doores, Katie J.; Le, Khoa; Sok, Devin; van Gils, Marit J.; Euler, Zelda; Burger, Judith A.; Seaman, Michael S.; Sanders, Rogier W.; Schuitemaker, Hanneke; Poignard, Pascal; Wrin, Terri; Burton, Dennis R. (Public Library of Science, 2015)
      The broadly neutralizing HIV monoclonal antibodies (bnMAbs) PG9, PG16, PGT151, and PGT152 have been shown earlier to occasionally display an unusual virus neutralization profile with a non-sigmoidal slope and a plateau at ...
    • Increased frequencies of CD8+CD57+ T cells are associated with antibody neutralization breadth against HIV in viraemic controllers 

      Palmer, Christine D; Romero-Tejeda, Marisol; Scully, Eileen P; Lockhart, Ainsley; Seaman, Michael S; Goldenthal, Ariel; Piechocka-Trocha, Alicja; Walker, Bruce D; Chibnik, Lori B; Jost, Stephanie; Porichis, Filippos (International AIDS Society, 2016)
      Introduction: An effective prophylactic vaccine against HIV will need to elicit antibody responses capable of recognizing and neutralizing rapidly evolving antigenic regions. The immunologic milieu associated with development ...
    • Inhibition of HCV by the serpin antithrombin III 

      Asmal, Mohammed; Seaman, Michael S.; Lin, Wenyu; Chung, Raymond Taeyong; Letvin, Norman L; Geiben-Lynn, Ralf (BioMed Central, 2012)
      Background: Although there have been dramatic strides made recently in the treatment of chronic hepatitis C virus infection, interferon-α based therapy remains challenging for certain populations, including those with ...
    • LabKey Server NAb: A Tool for Analyzing, Visualizing and Sharing Results from Neutralizing Antibody Assays 

      Piehler, Britt; Nelson, Elizabeth K.; Eckels, Josh; Ramsay, Sarah; Lum, Karl; Wood, Blake; Greene, Kelli M.; Gao, Hongmei; Montefiori, David C.; Igra, Mark; Seaman, Michael S. (BioMed Central, 2011)
      Background: Multiple types of assays allow sensitive detection of virus-specific neutralizing antibodies. For example, the extent of antibody neutralization of HIV-1, SIV and SHIV can be measured in the TZM-bl cell line ...
    • Molecular Evolution of Broadly Neutralizing Llama Antibodies to the CD4-Binding Site of HIV-1 

      McCoy, Laura E.; Rutten, Lucy; Frampton, Dan; Anderson, Ian; Granger, Luke; Bashford-Rogers, Rachael; Dekkers, Gillian; Strokappe, Nika M.; Seaman, Michael S.; Koh, Willie; Grippo, Vanina; Kliche, Alexander; Verrips, Theo; Kellam, Paul; Fassati, Ariberto; Weiss, Robin A. (Public Library of Science, 2014)
      To date, no immunization of humans or animals has elicited broadly neutralizing sera able to prevent HIV-1 transmission; however, elicitation of broad and potent heavy chain only antibodies (HCAb) has previously been ...
    • A New Glycan-Dependent CD4-Binding Site Neutralizing Antibody Exerts Pressure on HIV-1 In Vivo 

      Freund, Natalia T.; Horwitz, Joshua A.; Nogueira, Lilian; Sievers, Stuart A.; Scharf, Louise; Scheid, Johannes F.; Gazumyan, Anna; Liu, Cassie; Velinzon, Klara; Goldenthal, Ariel; Sanders, Rogier W.; Moore, John P.; Bjorkman, Pamela J.; Seaman, Michael S.; Walker, Bruce D.; Klein, Florian; Nussenzweig, Michel C. (Public Library of Science, 2015)
      The CD4 binding site (CD4bs) on the envelope glycoprotein is a major site of vulnerability that is conserved among different HIV-1 isolates. Many broadly neutralizing antibodies (bNAbs) to the CD4bs belong to the VRC01 ...
    • P03-07. Autologous Neutralizing Antibodies That Select Viral Escape Variants Emerge Late After SIV Infection of Rhesus Monkeys 

      Rahman, I; Hraber, P; Giri, A; Nevidomskyte, D; Coffey, RT; Miljkovic, S; Keele, BF; Shaw, GM; Korber, BT; Yeh, Wendy Wen-Li; Asmal, Mohammed; Whitney, James B.; Seaman, Michael S.; Letvin, Norman Lee (BioMed Central, 2009)