Browsing by Author "Wagner, Gerhard"
Now showing items 1-20 of 26
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4EGI-1 targets breast cancer stem cells by selective inhibition of translation that persists in CSC maintenance, proliferation and metastasis
Yi, Tingfang; Kabha, Eihab; Papadopoulos, Evangelos; Wagner, Gerhard (Impact Journals LLC, 2014)Cancer death is a leading cause of global mortality. An estimated 14.1 million new cancer cases and 8.2 million cancer deaths occurred worldwide in 2012 alone. Cancer stem cells (CSCs) within tumors are essential for tumor ... -
Amplification of phosphoglycerate dehydrogenase diverts glycolytic flux and contributes to oncogenesis
Locasale, J.; Grassian, Alexandra; Beroukhim, Rameen; Meyerson, Matthew Langer; Wagner, Gerhard; Asara, John M.; Brugge, Joan S.; Vander Heiden, Matthew George; Cantley, Lewis C. (BioMed Central, 2012) -
Complexes of Native Ubiquitin and Dodecyl Sulfate Illustrate the Nature of Hydrophobic and Electrostatic Interactions in the Binding of Proteins and Surfactants
Shaw, Bryan F.; Schneider, Grégory F.; Arthanari, Haribabu; Narovlyansky, Max; Moustakas, Demetri; Durazo, Armando; Wagner, Gerhard; Whitesides, George McClelland (American Chemical Society (ACS), 2011)A previous study, using capillary electrophoresis (CE) [J. Am. Chem. Soc.2008, 130, 17384–17393], reported that six discrete complexes of ubiquitin (UBI) and sodium dodecyl sulfate (SDS) form at different concentrations ... -
Design of Mixing Pulses for NMR Spectroscopy by Repeated Rotating Frames
Coote, Paul William (2014-06-06)In protein NMR spectroscopy, homonuclear mixing pulses are used to reveal correlations amongst chemically bonded nuclear spins. -
Discovery and Characterization of a Disulfide-Locked C2-Symmetric Defensin Peptide
Wommack, Andrew J.; Ziarek, Joshua J.; Tomaras, Jill; Chileveru, Haritha R.; Zhang, Yunfei; Wagner, Gerhard; Nolan, Elizabeth M. (American Chemical Society, 2014)We report the discovery of HD5-CD, an unprecedented C2-symmetric β-barrel-like covalent dimer of the cysteine-rich host-defense peptide human defensin 5 (HD5). Dimerization results from intermonomer disulfide exchange ... -
eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference
Yi, Tingfang; Arthanari, Haribabu; Akabayov, Barak; Song, Huaidong; Papadopoulos, Evangelos; Qi, Hank H.; Jedrychowski, Mark; Güttler, Thomas; Guo, Cuicui; Luna, Rafael E.; Gygi, Steven P.; Huang, Stephen A.; Wagner, Gerhard (2015)MicroRNA (miRNA) biogenesis and miRNA-guided RNA interference (RNAi) are essential for gene expression in eukaryotes. Here we report that translation initiation factor eIF1A directly interacts with Ago2 and promotes Ago2 ... -
Essential role of eIF5-mimic protein in animal development is linked to control of ATF4 expression
Hiraishi, Hiroyuki; Oatman, Jamie; Haller, Sherry L.; Blunk, Logan; McGivern, Benton; Morris, Jacob; Papadopoulos, Evangelos; Gutierrez, Wade; Gordon, Michelle; Bokhari, Wahaj; Ikeda, Yuka; Miles, David; Fellers, John; Asano, Masayo; Wagner, Gerhard; Tazi, Loubna; Rothenburg, Stefan; Brown, Susan J.; Asano, Katsura (Oxford University Press, 2014)Translational control of transcription factor ATF4 through paired upstream ORFs (uORFs) plays an important role in eukaryotic gene regulation. While it is typically induced by phosphorylation of eIF2α, ATF4 translation can ... -
Evolutionary Changes in the Leishmania eIF4F Complex Involve Variations in the eIF4E–eIF4G Interactions
Yoffe, Yael; Léger, Mélissa; Zinoviev, Alexandra; Zuberek, Joanna; Darzynkiewicz, Edward; Wagner, Gerhard; Shapira, Michal (Oxford University Press, 2009)Translation initiation in eukaryotes is mediated by assembly of the eIF4F complex over the m7GTP cap structure at the 5′-end of mRNAs. This requires an interaction between eIF4E and eIF4G, two eIF4F subunits. The Leishmania ... -
Human Translation Initiation Factor eIF4G1 Possesses a Low-Affinity ATP Binding Site Facing the ATP-Binding Cleft of eIF4A in the eIF4G/eIF4A Complex
Akabayov, Sabine R.; Akabayov, Barak; Wagner, Gerhard (American Chemical Society, 2014)Eukaryotic translation initiation factor 4G (eIF4G) plays a crucial role in translation initiation, serving as a scaffolding protein binding several other initiation factors, other proteins, and RNA. Binding of eIF4G to ... -
Identification of DNA primase inhibitors via a combined fragment-based and virtual screening
Ilic, Stefan; Akabayov, Sabine R.; Arthanari, Haribabu; Wagner, Gerhard; Richardson, Charles C.; Akabayov, Barak (Nature Publishing Group, 2016)The structural differences between bacterial and human primases render the former an excellent target for drug design. Here we describe a technique for selecting small molecule inhibitors of the activity of T7 DNA primase, ... -
Magic Angle Spinning Nuclear Magnetic Resonance Characterization of Voltage-Dependent Anion Channel Gating in Two-Dimensional Lipid Crystalline Bilayers
Eddy, Matthew T.; Andreas, Loren; Teijido, Oscar; Su, Yongchao; Clark, Lindsay; Noskov, Sergei Y.; Wagner, Gerhard; Rostovtseva, Tatiana K.; Griffin, Robert G. (American Chemical Society, 2014)The N-terminus of the voltage-dependent anion channel (VDAC) has been proposed to contain the mechanistically important gating helices that modulate channel opening and closing. In this study, we utilize magic angle spinning ... -
Molecular Signatures of Hemagglutinin Stem-Directed Heterosubtypic Human Neutralizing Antibodies against Influenza A Viruses
Avnir, Yuval; Tallarico, Aimee S.; Zhu, Quan; Bennett, Andrew S.; Connelly, Gene; Sheehan, Jared; Sui, Jianhua; Fahmy, Amr; Huang, Chiung-yu; Cadwell, Greg; Bankston, Laurie A.; McGuire, Andrew T.; Stamatatos, Leonidas; Wagner, Gerhard; Liddington, Robert C.; Marasco, Wayne A. (Public Library of Science, 2014)Recent studies have shown high usage of the IGHV1-69 germline immunoglobulin gene for influenza hemagglutinin stem-directed broadly-neutralizing antibodies (HV1-69-sBnAbs). Here we show that a major structural solution for ... -
Neutralizing positive charges at the surface of a protein lowers its rate of amide hydrogen exchange without altering its structure or increasing its thermostability
Whitesides, George M.; Shaw, Bryan F.; Arthanari, Haribabu; Lee, Andrew; Durazo, Armando; Frueh, Dominique P.; Pollastri, Michael P.; Bilgicer, Basar; Gygi, Steven P.; Wagner, Gerhard; Narovlyansky, Max (American Chemical Society, 2010)This paper combines two techniques—mass spectrometry and protein charge ladders—to examine the relationship between the surface charge and hydrophobicity of a protein (bovine carbonic anhydrase II; BCA II) and its rate of ... -
Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation
Kozel, Caitlin; Thompson, Brytteny; Hustak, Samantha; Moore, Chelsea; Nakashima, Akio; Singh, Chingakham Ranjit; Reid, Megan; Cox, Christian; Papadopoulos, Evangelos; Luna, Rafael E.; Anderson, Abbey; Tagami, Hideaki; Hiraishi, Hiroyuki; Slone, Emily Archer; Yoshino, Ken-ichi; Asano, Masayo; Gillaspie, Sarah; Nietfeld, Jerome; Perchellet, Jean-Pierre; Rothenburg, Stefan; Masai, Hisao; Wagner, Gerhard; Beeser, Alexander; Kikkawa, Ushio; Fleming, Sherry D.; Asano, Katsura (Oxford University Press, 2016)ATF4 is a pro-oncogenic transcription factor whose translation is activated by eIF2 phosphorylation through delayed re-initiation involving two uORFs in the mRNA leader. However, in yeast, the effect of eIF2 phosphorylation ... -
Position of Eukaryotic Translation Initiation Factor eIF1A on the 40S Ribosomal Subunit Mapped by Directed Hydroxyl Radical Probing
Yu, Yingpu; Marintchev, Assen; Kolupaeva, Victoria G.; Unbehaun, Anett; Veryasova, Tatyana; Lai, Shao-Chiang; Hong, Peng; Wagner, Gerhard; Hellen, Christopher U. T.; Pestova, Tatyana V. (Oxford University Press, 2009)The universally conserved eukaryotic initiation factor (eIF), eIF1A, plays multiple roles throughout initiation: it stimulates eIF2/GTP/Met-tRNA_i^{Met} attachment to 40S ribosomal subunits, scanning, start codon selection ... -
Selective Methyl Labeling of Eukaryotic Membrane Proteins Using Cell-Free Expression
Linser, Rasmus; Gelev, Vladimir; Hagn, Franz; Arthanari, Haribabu; Hyberts, Sven G.; Wagner, Gerhard (American Chemical Society, 2014)Structural characterization of membrane proteins and other large proteins with NMR relies increasingly on perdeuteration combined with incorporation of specifically protonated amino acid moieties, such as methyl groups of ... -
Selective Pharmacological Targeting of a DEAD Box RNA Helicase
Lindqvist, Lisa; Oberer, Monika; Reibarkh, Mikhail; Cencic, Regina; Bordeleau, Marie-Eve; Vogt, Emily; Marintchev, Assen; Tanaka, Junichi; Fagotto, Francois; Altmann, Michael; Pelletier, Jerry; Wagner, Gerhard (Public Library of Science, 2008)RNA helicases represent a large family of proteins implicated in many biological processes including ribosome biogenesis, splicing, translation and mRNA degradation. However, these proteins have little substrate specificity, ... -
Solid-State NMR Structure Determination from Diagonal-Compensated, Sparsely Nonuniform-Sampled 4D Proton–Proton Restraints
Linser, Rasmus; Bardiaux, Benjamin; Andreas, Loren B.; Hyberts, Sven G.; Morris, Vanessa K.; Pintacuda, Guido; Sunde, Margaret; Kwan, Ann H.; Wagner, Gerhard (American Chemical Society, 2014)We report acquisition of diagonal-compensated protein structural restraints from four-dimensional solid-state NMR spectra on extensively deuterated and 1H back-exchanged proteins. To achieve this, we use homonuclear 1H–1H ... -
Solution Structure of the Cuz1 AN1 Zinc Finger Domain: An Exposed LDFLP Motif Defines a Subfamily of AN1 Proteins
Sun, Zhen-Yu J.; Bhanu, Meera K.; Allan, Martin G.; Arthanari, Haribabu; Wagner, Gerhard; Hanna, John (Public Library of Science, 2016)Zinc binding domains are common and versatile protein structural motifs that mediate diverse cellular functions. Among the many structurally distinct families of zinc finger (ZnF) proteins, the AN1 domain remains poorly ... -
Structural Features of the αβTCR Mechanotransduction Apparatus That Promote pMHC Discrimination
Brazin, Kristine N.; Mallis, Robert J.; Das, Dibyendu Kumar; Feng, Yinnian; Hwang, Wonmuk; Wang, Jia-huai; Wagner, Gerhard; Lang, Matthew J.; Reinherz, Ellis L. (Frontiers Media S.A., 2015)The αβTCR was recently revealed to function as a mechanoreceptor. That is, it leverages mechanical energy generated during immune surveillance and at the immunological synapse to drive biochemical signaling following ...