Now showing items 1-5 of 5

    • Base editing of haematopoietic stem cells rescues sickle cell disease in mice 

      Newby, Gregory; Yen, Jonathan S.; Woodard, Kaitly J.; Mayuranathan, Thiyagaraj; Lazzarotto, Cicera R.; Li, Yichao; Sheppard-Tillman, Heather; Porter, Shaina N.; Yao, Yu; Mayberry, Kalin; Everette, Kelcee A.; Jang, Yoonjeong; Podracky, Christopher J.; Thaman, Elizabeth; Lechauve, Christophe; Sharma, Akshay; Henderson, Jordana M.; Richter, Michelle; Zhao, Kevin; Miller, Shannon; Wang, Tina; Koblan, Luke; McCaffrey, Anton P.; Tisdale, John F.; Kalfa, Theodosia; Pruett-Miller, Shondra M.; Tsai, Shengdar; Weiss, Mitchell J.; Liu, David (Springer Science and Business Media LLC, 2021-06-02)
    • Continuous Evolution of Base Editors With Expanded Target Compatibility and Improved Activity 

      Zheng, Christine; Wilson, Christopher; Thuronyi, Benjamin; Koblan, Luke; Levy, Jonathan; Yeh, Wei-Hsi; Newby, Gregory; Bhaumik, Mantu; Shubina-Oleinik, Olga; Holt, Jeffrey; Liu, David (Springer Science and Business Media LLC, 2019-07-22)
      Base editors use DNA-modifying enzymes targeted with a catalytically impaired CRISPR protein to precisely install point mutations. Here, we develop phage-assisted continuous evolution of base editors (BE–PACE) to improve ...
    • Efficient C•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning 

      Koblan, Luke; Arbab, Mandana; Shen, Max; Hussmann, Jeffrey A.; Anzalone, Andrew; Doman, Jordan; Newby, Gregory; Yang, Dian; Mok, Beverly; Replogle, Joseph M.; Xu, Albert; Sisley, Tyler A.; Weissman, Jonathan S.; Adamson, Brittany; Liu, David (Springer Science and Business Media LLC, 2021-06-28)
    • Engineered pegRNAs improve prime editing efficiency 

      Nelson, James W.; Randolph, Peyton B.; Shen, Simon; Everette, Kelcee A.; Chen, Peter; Anzalone, Andrew; An, Meirui; Newby, Gregory; Chen, Jonathan; Hsu, Alvin; Liu, David (Springer Science and Business Media LLC, 2021-10-04)
      Prime editing enables the installation of virtually any combination of point mutations, small insertions or small deletions in the DNA of living cells. A prime editing guide RNA (pegRNA) directs the prime editor protein ...
    • Search-and-Replace Genome Editing Without Double-Strand Breaks or Donor DNA 

      Anzalone, Andrew; Randolph, Peyton B.; Davis, Jessie R.; Sousa, Alexander; Koblan, Luke; Levy, Jonathan; Chen, Peter; Wilson, Christopher; Newby, Gregory; Raguram, Aditya; Liu, David R. (Springer Science and Business Media LLC, 2019-10-21)