Browsing by Author "Montague, Tessa"

Now showing items 1-6 of 6

    • Antisense Oligonucleotide-Mediated Transcript Knockdown in Zebrafish 

      Pauli, Andrea; Montague, Tessa G.; Lennox, Kim A.; Behlke, Mark A.; Schier, Alexander F. (Public Library of Science, 2015)
      Antisense oligonucleotides (ASOs) are synthetic, single-strand RNA-DNA hybrids that induce catalytic degradation of complementary cellular RNAs via RNase H. ASOs are widely used as gene knockdown reagents in tissue culture ...

    • Antisense Oligonucleotide-Mediated Transcript Knockdown in Zebrafish 

      Pauli, Andrea; Montague, Tessa G.; Lennox, Kim A.; Behlke, Mark A.; Schier, Alexander F. (Public Library of Science, 2015)
      Antisense oligonucleotides (ASOs) are synthetic, single-strand RNA-DNA hybrids that induce catalytic degradation of complementary cellular RNAs via RNase H. ASOs are widely used as gene knockdown reagents in tissue culture ...

    • CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering 

      Labun, Kornel; Montague, Tessa G.; Gagnon, James A.; Thyme, Summer B.; Valen, Eivind (Oxford University Press, 2016)
      In just 3 years CRISPR genome editing has transformed biology, and its popularity and potency continue to grow. New CRISPR effectors and rules for locating optimum targets continue to be reported, highlighting the need for ...

    • CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing 

      Montague, Tessa G.; Cruz, José M.; Gagnon, James A.; Church, George M.; Valen, Eivind (Oxford University Press, 2014)
      Major advances in genome editing have recently been made possible with the development of the TALEN and CRISPR/Cas9 methods. The speed and ease of implementing these technologies has led to an explosion of mutant and ...

    • Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs 

      Gagnon, James A.; Valen, Eivind; Thyme, Summer B.; Huang, Peng; Ahkmetova, Laila; Pauli, Andrea; Montague, Tessa G.; Zimmerman, Steven; Richter, Constance; Schier, Alexander F. (Public Library of Science, 2014)
      The CRISPR/Cas9 system has been implemented in a variety of model organisms to mediate site-directed mutagenesis. A wide range of mutation rates has been reported, but at a limited number of genomic target sites. To uncover ...

    • Internal guide RNA interactions interfere with Cas9-mediated cleavage 

      Thyme, Summer B.; Akhmetova, Laila; Montague, Tessa G.; Valen, Eivind; Schier, Alexander F. (Nature Publishing Group, 2016)
      The CRISPR/Cas system uses guide RNAs (gRNAs) to direct sequence-specific DNA cleavage. Not every gRNA elicits cleavage and the mechanisms that govern gRNA activity have not been resolved. Low activity could result from ...