Now showing items 1-19 of 19

    • Activin Signaling Mediates Muscle-to-Adipose Communication in a Mitochondria Dysfunction-Associated Obesity Model 

      Song, Wei; Owusu-Ansah, Edward; Hu, Yanhui; Cheng, Daojun; Ni, Xiaochun; Zirin, Jonathan; Perrimon, Norbert (National Academy of Sciences, 2017-08-08)
      Mitochondrial perturbation-associated dysregulation of one organ has been shown to nonautonomously affect the functions of other organs in both vertebrates and invertebrates. Using Drosophila as a genetic model organism, ...
    • Combining Genetic Perturbations and Proteomics to Examine Kinase-Phosphatase Networks in Drosophila Embryos 

      Sopko, Richelle; Foos, Marianna; Vinayagam, Arunachalam; Zhai, Bo; Binari, Richard; Hu, Yanhui; Randklev, Sakara; Perkins, Lizabeth A.; Gygi, Steven; Perrimon, Norbert (Elsevier BV, 2014-10-13)
      Connecting phosphorylation events to kinases and phosphatases is key to understanding the molecular organization and signaling dynamics of networks. We have generated a validated set of transgenic RNA-interference reagents ...
    • Conserved Regulators of Nucleolar Size Revealed by Global Phenotypic Analyses 

      Neumuller, Ralph; Gross, Thomas; Samsonova, Anastasia A.; Vinayagam, Arunachalam; Buckner, Michael; Founk, Karen; Hu, Yanhui; Sharifpoor, Sara; Rosebrock, Adam P.; Andrews, Brenda; Winston, Fred; Perrimon, Norbert (American Association for the Advancement of Science (AAAS), 2013-08-20)
      Regulation of cell growth is a fundamental process in development and disease that integrates a vast array of extra- and intracellular information. A central player in this process is RNA polymerase I (Pol I), which ...
    • Discovery of Progenitor Cell Signatures by Time-Series Synexpression Analysis During Drosophila Embryonic Cell Immortalization 

      Dequeant, Mary-Lee; Fagegaltier, Delphine; Hu, Yanhui; Spirohn, Kerstin; Simcox, Amanda; Hannon, Gregory J.; Perrimon, Norbert (National Academy of Sciences, 2015-10-20)
      The use of time series profiling to identify groups of functionally related genes (synexpression groups) is a powerful approach for the discovery of gene function. Here we apply this strategy during RasV12 immortalization ...
    • FlyBase at 25: looking to the future 

      Gramates, L. Sian; Marygold, Steven J.; dos Santos, Gilberto; Urbano, Jose-Maria; Antonazzo, Giulia; Matthews, Beverley B.; Rey, Alix J.; Tabone, Christopher J.; Crosby, Madeline A.; Emmert, David B.; Falls, Kathleen; Goodman, Joshua L.; Hu, Yanhui; Ponting, Laura; Schroeder, Andrew J.; Strelets, Victor B.; Thurmond, Jim; Zhou, Pinglei (Oxford University Press, 2017)
      Since 1992, FlyBase (flybase.org) has been an essential online resource for the Drosophila research community. Concentrating on the most extensively studied species, Drosophila melanogaster, FlyBase includes information ...
    • FlyRNAi.org—the database of the Drosophila RNAi screening center and transgenic RNAi project: 2017 update 

      Hu, Yanhui; Comjean, Aram; Roesel, Charles; Vinayagam, Arunachalam; Flockhart, Ian; Zirin, Jonathan; Perkins, Lizabeth; Perrimon, Norbert; Mohr, Stephanie E. (Oxford University Press, 2017)
      The FlyRNAi database of the Drosophila RNAi Screening Center (DRSC) and Transgenic RNAi Project (TRiP) at Harvard Medical School and associated DRSC/TRiP Functional Genomics Resources website (http://fgr.hms.harvard.edu) ...
    • FlyRNAi.org—The Database of the Drosophila RNAi Screening Center: 2012 Update 

      Flockhart, Ian; Booker, Matthew; Hu, Yanhui; McElvany, Benjamin D; Gilly, Quentin Carl; Mathey-Prevot, Bernard; Perrimon, Norbert; Mohr, Stephanie (Oxford University Press, 2011)
      FlyRNAi (http://www.flyrnai.org), the database and website of the Drosophila RNAi Screening Center (DRSC) at Harvard Medical School, serves a dual role, tracking both production of reagents for RNA interference (RNAi) ...
    • A Full-Genomic Sequence-Verified Protein-Coding Gene Collection for Francisella tularensis 

      Murthy, Tal; Raphael, Jacob; Moreira, Donna; Kelley, Fontina; Taycher, Elena; Brizuela, Leonardo; LaBaer, Joshua; Petrosino, Joseph; Rolfs, Andreas; Hu, Yanhui; McCarron, Seamus; Zuo, Dongmei; Shi, Zhenwei; Jepson, Daniel Ashley; Mohr, Stephanie; Fernandez, Mauricio J. (Public Library of Science, 2007)
      The rapid development of new technologies for the high throughput (HT) study of proteins has increased the demand for comprehensive plasmid clone resources that support protein expression. These clones must be full-length, ...
    • Genetic Determinants of Phosphate Response in Drosophila 

      Bergwitz, Clemens; Wee, Mark J.; Sinha, Sumi; Huang, Joanne Hyunjung; DeRobertis, Charles; Mensah, Lawrence; Cohen, Jonathan Brewer; Friedman, Adam Amiel Laufer; Kulkarni, Meghana; Hu, Yanhui; Vinayagam, Arunachalam; Schnall-Levin, Michael; Berger, Bonnie; Perkins, Lizabeth A.; Mohr, Stephanie; Perrimon, Norbert (Public Library of Science, 2013)
      Phosphate is required for many important cellular processes and having too little phosphate or too much can cause disease and reduce life span in humans. However, the mechanisms underlying homeostatic control of extracellular ...
    • Integrating protein-protein interaction networks with phenotypes reveals signs of interactions 

      Vinayagam, Arunachalam; Zirin, Jonathan; Roesel, Charles; Hu, Yanhui; Yilmazel, Bahar; Samsonova, Anastasia A.; Neumüller, Ralph A.; Mohr, Stephanie E.; Perrimon, Norbert (2013)
      A major objective of systems biology is to organize molecular interactions as networks and to characterize information-flow within networks. We describe a computational framework to integrate protein-protein interaction ...
    • iProteinDB: An Integrative Database of Drosophila Post-translational Modifications 

      Hu, Yanhui; Sopko, Richelle; Chung, Verena; Foos, Marianna; Studer, Romain A.; Landry, Sean D.; Liu, Daniel; Rabinow, Leonard; Gnad, Florian; Beltrao, Pedro; Perrimon, Norbert (Genetics Society of America, 2018-11-05)
      Post-translational modification (PTM) serves as a regulatory mechanism for protein function, influencing their stability, interactions, activity and localization, and is critical in many signaling pathways. The best ...
    • Mapping Signaling Pathway Cross-Talk in Drosophila Cells 

      Ammeux, Noemie; Housden, Benjamin; Georgiadis, Andrew; Hu, Yanhui; Perrimon, Norbert (National Academy of Sciences, 2016-08-30)
      A key challenge in understanding cell communication is to characterize the coordinated activity of signaling pathways. A number of studies suggest that signaling pathways can regulate each other by direct control of ligand ...
    • A Novel Approach to Sequence Validating Protein Expression Clones with Automated Decision Making 

      Taycher, Elena; Rolfs, Andreas; Hu, Yanhui; Zuo, Dongmei; Mohr, Stephanie; Williamson, Janice; LaBaer, Joshua (BioMed Central, 2007)
      Background: Whereas the molecular assembly of protein expression clones is readily automated and routinely accomplished in high throughput, sequence verification of these clones is still largely performed manually, an ...
    • Online GESS: prediction of miRNA-like off-target effects in large-scale RNAi screen data by seed region analysis 

      Yilmazel, Bahar; Hu, Yanhui; Sigoillot, Frederic; Smith, Jennifer A; Shamu, Caroline E; Perrimon, Norbert; Mohr, Stephanie E (BioMed Central, 2014)
      Background: RNA interference (RNAi) is an effective and important tool used to study gene function. For large-scale screens, RNAi is used to systematically down-regulate genes of interest and analyze their roles in a ...
    • Optimized Gene Editing Technology for Drosophila melanogaster Using Germ Line-Specific Cas9 

      Ren, Xingjie; Sun, Jin; Housden, Benjamin; Hu, Yanhui; Roesel, Charles; Lin, Shuailiang; Liu, Lu-Ping; Yang, Zhihao; Mao, Decai; Sun, Lingzhu; Wu, Qujie; Ji, Jun-Yuan; Xi, Jianzhong; Mohr, Stephanie; Xu, Jiang; Perrimon, Norbert; Ni, Jian-Quan (National Academy of Sciences, 2013-11-19)
      The ability to engineer genomes in a specific, systematic, and cost- effective way is critical for functional genomic studies. Recent advances using the CRISPR-associated single-guide RNA system (Cas9/sgRNA) illustrate the ...
    • Optimized Strategy for in Vivo Cas9-Activation in Drosophila 

      Ewen-Campen, Benjamin; Yang-Zhou, Donghui; Fernandes, Vitória R.; González, Delfina P.; Liu, Lu-Ping; Tao, Rong; Ren, Xingjie; Sun, Jin; Hu, Yanhui; Zirin, Jonathan; Mohr, Stephanie; Ni, Jian-Quan; Perrimon, Norbert (National Academy of Sciences, 2017-08-29)
      While several large-scale resources are available for in vivo loss-of-function studies in Drosophila, an analogous resource for overexpressing genes from their endogenous loci does not exist. We describe a strategy for ...
    • Pooled Genome-Wide CRISPR Screening for Basal and Context-Specific Fitness Gene Essentiality in Drosophila Cells 

      Viswanatha, Raghuvir; Li, Zhongchi; Hu, Yanhui; Perrimon, Norbert (eLife Sciences Publications, Ltd, 2018-07-27)
      Genome-wide screens in Drosophila cells have offered numerous insights into gene function, yet a major limitation has been the inability to stably deliver large multiplexed DNA libraries to cultured cells allowing barcoded ...
    • Protein Structure Initiative Material Repository: an Open Shared Public Resource of Structural Genomics Plasmids for the Biological Community 

      Cormier, Catherine Y.; Kramer, Jason; Taycher, Elena; Kelley, Fontina; Fiacco, Michael; Turnbull, Greggory; LaBaer, Joshua; Mohr, Stephanie; Zuo, Dongmei; Hu, Yanhui; Rolfs, Andreas (Oxford University Press, 2009)
      The Protein Structure Initiative Material Repository (PSI-MR; http://psimr.asu.edu) provides centralized storage and distribution for the protein expression plasmids created by PSI researchers. These plasmids are a resource ...
    • The Septate Junction Protein Tsp2A Restricts Intestinal Stem Cell Activity via Endocytic Regulation of aPKC and Hippo Signaling 

      Xu, Chiwei; Tang, Hong-Wen; Hung, Ruei-Jiun; Hu, Yanhui; Ni, Xiaochun; Housden, Benjamin; Perrimon, Norbert (Elsevier BV, 2019-01-15)
      Hippo signaling and the activity of its transcriptional coactivator, Yorkie (Yki), are conserved and crucial regulators of tissue homeostasis. In the Drosophila midgut, after tissue damage, Yki activity increases to stimulate ...