Now showing items 1-10 of 10

    • Automated motion artifact removal for intravital microscopy, without a priori information 

      Lee, Sungon; Vinegoni, Claudio; Sebas, Matthew; Weissleder, Ralph (Nature Publishing Group, 2014)
      Intravital fluorescence microscopy, through extended penetration depth and imaging resolution, provides the ability to image at cellular and subcellular resolution in live animals, presenting an opportunity for new insights ...
    • Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging 

      Vinegoni, Claudio; Razansky, Daniel; Figueiredo, Jose-Luiz; Fexon, Lyuba; Pivovarov, Misha; Nahrendorf, Matthias; Ntziachristos, Vasilis; Weissleder, Ralph (MyJove Corporation, 2009)
      Optical projection tomography is a three-dimensional imaging technique that has been recently introduced as an imaging tool primarily in developmental biology and gene expression studies. The technique renders biological ...
    • Chronic variable stress activates hematopoietic stem cells 

      Heidt, Timo; Sager, Hendrik B.; Courties, Gabriel; Dutta, Partha; Iwamoto, Yoshiko; Zaltsman, Alex; von zur Muhlen, Constantin; Bode, Christoph; Fricchione, Gregory L.; Denninger, John; Lin, Charles P.; Vinegoni, Claudio; Libby, Peter; Swirski, Filip K.; Weissleder, Ralph; Nahrendorf, Matthias (2014)
      Exposure to psychosocial stress is a risk factor for many diseases, including atherosclerosis1,2. While incompletely understood, interaction between the psyche and the immune system provides one potential mechanism linking ...
    • Computational imaging reveals mitochondrial morphology as a biomarker of cancer phenotype and drug response 

      Giedt, Randy J.; Fumene Feruglio, Paolo; Pathania, Divya; Yang, Katherine S.; Kilcoyne, Aoife; Vinegoni, Claudio; Mitchison, Timothy J.; Weissleder, Ralph (Nature Publishing Group, 2016)
      Mitochondria, which are essential organelles in resting and replicating cells, can vary in number, mass and shape. Past research has primarily focused on short-term molecular mechanisms underlying fission/fusion. Less is ...
    • Mapping Molecular Agents Distributions in Whole Mice Hearts Using Born-Normalized Optical Projection Tomography 

      Vinegoni, Claudio; Feruglio, Paolo Fumene; Razansky, Daniel; Gorbatov, Rostic; Ntziachristos, Vasilis; Sbarbati, Andrea; Nahrendorf, Matthias; Weissleder, Ralph (Public Library of Science, 2012)
      To date there is a lack of tools to map the spatio-temporal dynamics of diverse cells in experimental heart models. Conventional histology is labor intensive with limited coverage, whereas many imaging techniques do not ...
    • Myocardial Infarction Accelerates Atherosclerosis 

      Leuschner, Florian; Robbins, Clinton; Iwamoto, Yoshiko; Thompson, Brian; Carlson, Alicia L.; Heidt, Timo; Lasitschka, Felix; Etzrodt, Martin; Waterman, Peter; Waring, Michael T.; Chicoine, Adam T.; van der Laan, Anja M.; Niessen, Hans W.M.; Piek, Jan J.; Rubin, Barry B.; Butany, Jagdish; Katus, Hugo A.; Murphy, Sabina A.; Pittet, Mikael J.; Lin, Charles P.; Dutta, Partha; Courties, Gabriel P; Wei, Ying; Gorbatov, Rostic; Majmudar, Maulik D; Stone, James Robert; Morrow, David Andrew; Sabatine, Marc Steven; Vinegoni, Claudio; Moskowitz, Michael Arthur; Libby, Peter; Swirski, Filip K.; Weissleder, Ralph; Nahrendorf, Matthias (Nature Publishing Group, 2012)
      During progression of atherosclerosis, myeloid cells destabilize lipid-rich plaque in the arterial wall and cause its rupture, thus triggering myocardial infarction and stroke. Survivors of acute coronary syndromes have a ...
    • Myocardial Infarction Activates CCR2+ Hematopoietic Stem and Progenitor Cells 

      Dutta, Partha; Sager, Hendrik B; Stengel, Kristy R.; Naxerova, Kamila; Courties, Gabriel P; Saez, Borja; Silberstein, Lev; Heidt, Timo; Sebas, Matthew; Sun, Yuan; Wojtkiewicz, Gregory; Feruglio, Paolo Fumene; King, Kevin Robert; Baker, Joshua N.; van der Laan, Anja M.; Borodovsky, Anna; Fitzgerald, Kevin; Hulsmans, Maarten; Hoyer, Friedrich Felix; Iwamoto, Yoshiko; Vinegoni, Claudio; Brown, Dennis; Di Carli, Marcelo; Libby, Peter; Hiebert, Scott W.; Scadden, David Thomas; Swirski, Filip K.; Weissleder, Ralph; Nahrendorf, Matthias (Elsevier BV, 2015)
      Following myocardial infarction (MI), myeloid cells derived from the hematopoietic system drive a sharp increase in systemic leukocyte levels that correlates closely with mortality. The origin of these myeloid cells, and ...
    • New techniques for motion-artifact-free in vivo cardiac microscopy 

      Vinegoni, Claudio; Lee, Sungon; Aguirre, Aaron D.; Weissleder, Ralph (Frontiers Media S.A., 2015)
      Intravital imaging microscopy (i.e., imaging in live animals at microscopic resolution) has become an indispensable tool for studying the cellular micro-dynamics in cancer, immunology and neurobiology. High spatial and ...
    • Rapid, high efficiency isolation of pancreatic ß-cells 

      Clardy, Susan M.; Mohan, James F.; Vinegoni, Claudio; Keliher, Edmund J.; Iwamoto, Yoshiko; Benoist, Christophe; Mathis, Diane; Weissleder, Ralph (Nature Publishing Group, 2015)
      The ability to isolate pure pancreatic ß-cells would greatly aid multiple areas of diabetes research. We developed a fluorescent exendin-4-like neopeptide conjugate for the rapid purification and isolation of functional ...
    • Tyrosine kinase-mediated axial motility of basal cells revealed by intravital imaging 

      Roy, Jeremy; Kim, Bongki; Hill, Eric; Visconti, Pablo; Krapf, Dario; Vinegoni, Claudio; Weissleder, Ralph; Brown, Dennis; Breton, Sylvie (Nature Publishing Group, 2016)
      Epithelial cells are generally considered to be static relative to their neighbours. Basal cells in pseudostratified epithelia display a single long cytoplasmic process that can cross the tight junction barrier to reach ...